Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2121 and Shewana3_R0089 are separated by 293 nucleotides Shewana3_R0089 and Shewana3_R0090 are separated by 49 nucleotides Shewana3_R0090 and Shewana3_R0091 are separated by 23 nucleotides Shewana3_R0091 and Shewana3_2122 are separated by 325 nucleotides Shewana3_2122 and Shewana3_2123 are separated by 206 nucleotides
Shewana3_2121: Shewana3_2121 - aromatic amino acid aminotransferase (RefSeq), at 2,544,569 to 2,545,762
_2121
Shewana3_R0089: Shewana3_R0089 - tRNA-Leu (RefSeq), at 2,546,056 to 2,546,142
_R0089
Shewana3_R0090: Shewana3_R0090 - tRNA-Cys (RefSeq), at 2,546,192 to 2,546,265
_R0090
Shewana3_R0091: Shewana3_R0091 - tRNA-Gly (RefSeq), at 2,546,289 to 2,546,364
_R0091
Shewana3_2122: Shewana3_2122 - glyceraldehyde-3-phosphate dehydrogenase (RefSeq), at 2,546,690 to 2,548,123
_2122
Shewana3_2123: Shewana3_2123 - hypothetical protein (RefSeq), at 2,548,330 to 2,549,217
_2123
Position (kb)
2546
2547
2548
2549 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2545.695 kb on + strand at 2545.814 kb on - strand at 2545.826 kb on + strand at 2546.002 kb on - strand at 2546.042 kb on - strand at 2546.614 kb on + strand at 2546.624 kb on + strand at 2546.918 kb on - strand, within Shewana3_2122 at 2547.243 kb on - strand, within Shewana3_2122 at 2547.684 kb on - strand, within Shewana3_2122 at 2547.801 kb on - strand, within Shewana3_2122 at 2548.370 kb on + strand at 2548.790 kb on + strand, within Shewana3_2123 at 2548.939 kb on - strand, within Shewana3_2123 at 2549.080 kb on + strand, within Shewana3_2123 at 2549.080 kb on + strand, within Shewana3_2123
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 2,545,695 + -1.8 2,545,814 - -0.9 2,545,826 + -0.5 2,546,002 - -0.5 2,546,042 - +0.2 2,546,614 + +0.4 2,546,624 + -0.5 2,546,918 - Shewana3_2122 0.16 +1.4 2,547,243 - Shewana3_2122 0.39 +0.8 2,547,684 - Shewana3_2122 0.69 -0.5 2,547,801 - Shewana3_2122 0.77 +0.2 2,548,370 + +1.0 2,548,790 + Shewana3_2123 0.52 +2.5 2,548,939 - Shewana3_2123 0.69 +0.8 2,549,080 + Shewana3_2123 0.84 +0.0 2,549,080 + Shewana3_2123 0.84 +0.9
Or see this region's nucleotide sequence