Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1980 and Shewana3_1981 are separated by 42 nucleotides Shewana3_1981 and Shewana3_1982 are separated by 72 nucleotides Shewana3_1982 and Shewana3_1983 are separated by 59 nucleotides
Shewana3_1980: Shewana3_1980 - tetratricopeptide repeat protein (RefSeq), at 2,358,326 to 2,359,486
_1980
Shewana3_1981: Shewana3_1981 - orotidine 5'-phosphate decarboxylase (RefSeq), at 2,359,529 to 2,360,224
_1981
Shewana3_1982: Shewana3_1982 - short chain dehydrogenase (RefSeq), at 2,360,297 to 2,361,124
_1982
Shewana3_1983: Shewana3_1983 - hypothetical protein (RefSeq), at 2,361,184 to 2,361,726
_1983
Position (kb)
2359
2360
2361 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2359.707 kb on + strand, within Shewana3_1981 at 2360.093 kb on + strand, within Shewana3_1981 at 2360.475 kb on - strand, within Shewana3_1982 at 2360.536 kb on - strand, within Shewana3_1982 at 2360.969 kb on + strand, within Shewana3_1982 at 2361.043 kb on - strand at 2361.210 kb on + strand at 2361.210 kb on + strand at 2361.210 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 2,359,707 + Shewana3_1981 0.26 -4.6 2,360,093 + Shewana3_1981 0.81 -3.1 2,360,475 - Shewana3_1982 0.21 +0.6 2,360,536 - Shewana3_1982 0.29 -0.4 2,360,969 + Shewana3_1982 0.81 -0.7 2,361,043 - -0.3 2,361,210 + +0.6 2,361,210 + +0.9 2,361,210 + -2.3
Or see this region's nucleotide sequence