Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0050 and Shewana3_0051 are separated by 10 nucleotides Shewana3_0051 and Shewana3_0052 are separated by 223 nucleotides Shewana3_0052 and Shewana3_0053 are separated by 260 nucleotides
Shewana3_0050: Shewana3_0050 - peptidase M23B (RefSeq), at 59,082 to 60,215
_0050
Shewana3_0051: Shewana3_0051 - phosphoglyceromutase (RefSeq), at 60,226 to 61,770
_0051
Shewana3_0052: Shewana3_0052 - rhodanese domain-containing protein (RefSeq), at 61,994 to 62,470
_0052
Shewana3_0053: Shewana3_0053 - preprotein translocase subunit SecB (RefSeq), at 62,731 to 63,216
_0053
Position (kb)
60
61
62 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 59.301 kb on - strand, within Shewana3_0050 at 59.302 kb on - strand, within Shewana3_0050 at 60.109 kb on + strand at 60.211 kb on - strand at 61.862 kb on + strand at 61.870 kb on - strand at 61.900 kb on - strand at 61.928 kb on + strand at 61.984 kb on + strand at 61.992 kb on - strand at 62.034 kb on - strand at 62.080 kb on - strand, within Shewana3_0052 at 62.165 kb on + strand, within Shewana3_0052 at 62.180 kb on - strand, within Shewana3_0052 at 62.255 kb on + strand, within Shewana3_0052 at 62.332 kb on + strand, within Shewana3_0052 at 62.332 kb on + strand, within Shewana3_0052 at 62.484 kb on + strand at 62.494 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 59,301 - Shewana3_0050 0.19 +1.0 59,302 - Shewana3_0050 0.19 +0.5 60,109 + -3.2 60,211 - +0.0 61,862 + -0.2 61,870 - +0.2 61,900 - +0.5 61,928 + -0.2 61,984 + -0.4 61,992 - -0.9 62,034 - -0.8 62,080 - Shewana3_0052 0.18 -1.5 62,165 + Shewana3_0052 0.36 -0.6 62,180 - Shewana3_0052 0.39 +0.0 62,255 + Shewana3_0052 0.55 +0.4 62,332 + Shewana3_0052 0.71 -0.8 62,332 + Shewana3_0052 0.71 -0.8 62,484 + -0.0 62,494 + +0.0
Or see this region's nucleotide sequence