Experiment: L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2371 and Shew_2372 are separated by 11 nucleotides Shew_2372 and Shew_2373 are separated by 299 nucleotides Shew_2373 and Shew_2374 are separated by 237 nucleotides
Shew_2371: Shew_2371 - actP-like component of L-lactate and L-malate uptake system (from data) , at 2,772,719 to 2,774,437
_2371
Shew_2372: Shew_2372 - small component of L-lactate and L-malate uptake system (from data) , at 2,774,449 to 2,774,715
_2372
Shew_2373: Shew_2373 - DSBA oxidoreductase (RefSeq), at 2,775,015 to 2,775,647
_2373
Shew_2374: Shew_2374 - NAD-dependent epimerase/dehydratase (RefSeq), at 2,775,885 to 2,776,784
_2374
Position (kb)
2775
2776 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2774.095 kb on + strand, within Shew_2371 at 2774.163 kb on - strand, within Shew_2371 at 2774.542 kb on - strand, within Shew_2372 at 2774.731 kb on - strand at 2774.772 kb on + strand at 2774.984 kb on + strand at 2775.213 kb on - strand, within Shew_2373 at 2775.316 kb on - strand, within Shew_2373 at 2775.562 kb on + strand, within Shew_2373 at 2775.570 kb on - strand, within Shew_2373 at 2775.570 kb on - strand, within Shew_2373 at 2775.853 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate (C) remove 2,774,095 + Shew_2371 0.80 -3.5 2,774,163 - Shew_2371 0.84 -4.2 2,774,542 - Shew_2372 0.35 -5.2 2,774,731 - -2.9 2,774,772 + -3.8 2,774,984 + +0.5 2,775,213 - Shew_2373 0.31 -0.1 2,775,316 - Shew_2373 0.48 -0.4 2,775,562 + Shew_2373 0.86 +0.4 2,775,570 - Shew_2373 0.88 -0.9 2,775,570 - Shew_2373 0.88 -0.4 2,775,853 - +0.2
Or see this region's nucleotide sequence