Experiment: L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_R0078 and Shew_R0079 are separated by 93 nucleotides Shew_R0079 and Shew_R0080 are separated by 49 nucleotides Shew_R0080 and Shew_2110 are separated by 117 nucleotides Shew_2110 and Shew_2111 are separated by 10 nucleotides Shew_2111 and Shew_2112 are separated by 68 nucleotides
Shew_R0078: Shew_R0078 - tRNA-OTHER (NCBI), at 2,445,114 to 2,445,202
_R0078
Shew_R0079: Shew_R0079 - tRNA-Asp (RefSeq), at 2,445,296 to 2,445,372
_R0079
Shew_R0080: Shew_R0080 - tRNA-Asp (RefSeq), at 2,445,422 to 2,445,498
_R0080
Shew_2110: Shew_2110 - hypothetical protein (RefSeq), at 2,445,616 to 2,446,938
_2110
Shew_2111: Shew_2111 - DNA polymerase III, epsilon subunit (RefSeq), at 2,446,949 to 2,447,677
_2111
Shew_2112: Shew_2112 - ribonuclease H (RefSeq), at 2,447,746 to 2,448,222
_2112
Position (kb)
2445
2446
2447 Strain fitness (log2 ratio)
-1
0
1 at 2444.661 kb on + strand at 2445.818 kb on - strand, within Shew_2110 at 2445.824 kb on + strand, within Shew_2110 at 2445.824 kb on + strand, within Shew_2110 at 2445.832 kb on - strand, within Shew_2110 at 2445.905 kb on - strand, within Shew_2110 at 2446.856 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate (C) remove 2,444,661 + -0.6 2,445,818 - Shew_2110 0.15 +0.8 2,445,824 + Shew_2110 0.16 +0.7 2,445,824 + Shew_2110 0.16 +0.3 2,445,832 - Shew_2110 0.16 +0.7 2,445,905 - Shew_2110 0.22 +0.7 2,446,856 - +1.5
Or see this region's nucleotide sequence