Experiment: CDM1 without Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12415 and BBR_RS12420 are separated by 268 nucleotides BBR_RS12420 and BBR_RS12425 are separated by 91 nucleotides BBR_RS12425 and BBR_RS12430 are separated by 358 nucleotides
BBR_RS12415: BBR_RS12415 - permease, at 533,953 to 534,954
_RS12415
BBR_RS12420: BBR_RS12420 - tRNA-Leu, at 535,223 to 535,303
_RS12420
BBR_RS12425: BBR_RS12425 - tRNA-Thr, at 535,395 to 535,470
_RS12425
BBR_RS12430: BBR_RS12430 - glucose-6-phosphate isomerase, at 535,829 to 537,529
_RS12430
Position (kb) 535
536 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 534.403 kb on + strand, within BBR_RS12415 at 534.403 kb on + strand, within BBR_RS12415 at 534.409 kb on - strand, within BBR_RS12415 at 534.436 kb on - strand, within BBR_RS12415 at 534.444 kb on - strand, within BBR_RS12415 at 534.455 kb on + strand, within BBR_RS12415 at 534.463 kb on - strand, within BBR_RS12415 at 534.463 kb on - strand, within BBR_RS12415 at 534.474 kb on - strand, within BBR_RS12415 at 534.590 kb on + strand, within BBR_RS12415 at 534.598 kb on - strand, within BBR_RS12415 at 534.614 kb on + strand, within BBR_RS12415 at 534.624 kb on + strand, within BBR_RS12415 at 534.624 kb on + strand, within BBR_RS12415 at 534.662 kb on + strand, within BBR_RS12415 at 534.665 kb on + strand, within BBR_RS12415 at 534.670 kb on - strand, within BBR_RS12415 at 534.686 kb on - strand, within BBR_RS12415 at 534.686 kb on - strand, within BBR_RS12415 at 534.700 kb on - strand, within BBR_RS12415 at 534.702 kb on + strand, within BBR_RS12415 at 534.733 kb on + strand, within BBR_RS12415 at 534.733 kb on + strand, within BBR_RS12415 at 534.767 kb on + strand, within BBR_RS12415 at 534.769 kb on + strand, within BBR_RS12415 at 534.770 kb on - strand, within BBR_RS12415 at 534.770 kb on - strand, within BBR_RS12415 at 534.777 kb on + strand, within BBR_RS12415 at 534.813 kb on + strand, within BBR_RS12415 at 534.833 kb on + strand, within BBR_RS12415 at 534.833 kb on + strand, within BBR_RS12415 at 534.833 kb on + strand, within BBR_RS12415 at 534.841 kb on - strand, within BBR_RS12415 at 534.848 kb on - strand, within BBR_RS12415 at 534.890 kb on - strand at 534.903 kb on - strand at 534.908 kb on - strand at 534.948 kb on - strand at 534.950 kb on - strand at 534.963 kb on + strand at 535.023 kb on + strand at 535.031 kb on + strand at 535.031 kb on - strand at 535.063 kb on - strand at 535.157 kb on + strand at 535.210 kb on - strand at 535.211 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.212 kb on + strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.220 kb on - strand at 535.316 kb on + strand at 535.319 kb on - strand at 535.391 kb on - strand at 535.391 kb on - strand at 535.391 kb on - strand at 535.633 kb on + strand at 535.641 kb on - strand at 535.668 kb on + strand at 535.676 kb on - strand at 535.678 kb on - strand at 535.696 kb on - strand at 535.775 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Threonine remove 534,403 + BBR_RS12415 0.45 -0.8 534,403 + BBR_RS12415 0.45 -0.5 534,409 - BBR_RS12415 0.46 +0.1 534,436 - BBR_RS12415 0.48 +0.0 534,444 - BBR_RS12415 0.49 +0.8 534,455 + BBR_RS12415 0.50 -0.3 534,463 - BBR_RS12415 0.51 -0.2 534,463 - BBR_RS12415 0.51 +0.3 534,474 - BBR_RS12415 0.52 -0.3 534,590 + BBR_RS12415 0.64 -1.0 534,598 - BBR_RS12415 0.64 -0.4 534,614 + BBR_RS12415 0.66 -1.3 534,624 + BBR_RS12415 0.67 -1.4 534,624 + BBR_RS12415 0.67 -2.9 534,662 + BBR_RS12415 0.71 -0.1 534,665 + BBR_RS12415 0.71 -2.0 534,670 - BBR_RS12415 0.72 -0.2 534,686 - BBR_RS12415 0.73 -0.0 534,686 - BBR_RS12415 0.73 +0.3 534,700 - BBR_RS12415 0.75 -0.5 534,702 + BBR_RS12415 0.75 -0.9 534,733 + BBR_RS12415 0.78 -0.2 534,733 + BBR_RS12415 0.78 -0.5 534,767 + BBR_RS12415 0.81 -0.7 534,769 + BBR_RS12415 0.81 -2.7 534,770 - BBR_RS12415 0.82 +0.1 534,770 - BBR_RS12415 0.82 -0.2 534,777 + BBR_RS12415 0.82 -0.3 534,813 + BBR_RS12415 0.86 -1.4 534,833 + BBR_RS12415 0.88 -0.9 534,833 + BBR_RS12415 0.88 -3.0 534,833 + BBR_RS12415 0.88 +0.4 534,841 - BBR_RS12415 0.89 +1.2 534,848 - BBR_RS12415 0.89 -0.5 534,890 - -1.2 534,903 - -0.5 534,908 - +0.3 534,948 - +0.3 534,950 - -1.7 534,963 + -0.1 535,023 + -0.1 535,031 + +1.2 535,031 - +0.1 535,063 - +0.6 535,157 + -2.5 535,210 - +1.3 535,211 + -0.8 535,212 + -1.2 535,212 + -0.1 535,212 + -0.4 535,212 + -0.8 535,212 + -3.7 535,212 + -0.8 535,212 + -2.0 535,212 + -1.1 535,212 + +0.1 535,212 + -1.5 535,212 + -0.7 535,220 - +0.0 535,220 - -0.7 535,220 - -0.3 535,220 - -1.9 535,220 - +0.2 535,220 - -0.6 535,220 - -0.0 535,316 + -1.2 535,319 - -0.7 535,391 - -1.1 535,391 - -1.4 535,391 - -0.9 535,633 + -0.1 535,641 - -0.3 535,668 + -0.2 535,676 - -0.5 535,678 - -1.1 535,696 - -0.5 535,775 + -1.6
Or see this region's nucleotide sequence