Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_5000 and Ac3H11_5001 are separated by 412 nucleotides Ac3H11_5001 and Ac3H11_5002 are separated by 13 nucleotides Ac3H11_5002 and Ac3H11_5003 overlap by 4 nucleotides Ac3H11_5003 and Ac3H11_5004 are separated by 37 nucleotides
Ac3H11_5000 - 2-isopropylmalate synthase (EC 2.3.3.13), at 169,725 to 171,425
_5000
Ac3H11_5001 - PhnB protein, at 171,838 to 172,203
_5001
Ac3H11_5002 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 172,217 to 174,142
_5002
Ac3H11_5003 - putative transmembrane protein, at 174,139 to 174,657
_5003
Ac3H11_5004 - TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins, at 174,695 to 176,593
_5004
Position (kb) 172
173
174
175 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 171.400 kb on + strand at 171.400 kb on + strand at 171.400 kb on + strand at 171.400 kb on + strand at 171.400 kb on + strand at 171.400 kb on + strand at 171.401 kb on - strand at 171.401 kb on - strand at 171.622 kb on + strand at 171.622 kb on + strand at 171.622 kb on + strand at 171.622 kb on + strand at 171.622 kb on + strand at 171.623 kb on - strand at 171.623 kb on - strand at 171.634 kb on + strand at 171.664 kb on - strand at 171.677 kb on + strand at 171.678 kb on - strand at 171.844 kb on + strand at 171.844 kb on + strand at 171.845 kb on - strand at 171.845 kb on - strand at 171.845 kb on - strand at 171.845 kb on - strand at 171.913 kb on + strand, within Ac3H11_5001 at 171.913 kb on + strand, within Ac3H11_5001 at 171.913 kb on + strand, within Ac3H11_5001 at 171.913 kb on + strand, within Ac3H11_5001 at 171.914 kb on - strand, within Ac3H11_5001 at 171.914 kb on - strand, within Ac3H11_5001 at 171.914 kb on - strand, within Ac3H11_5001 at 171.914 kb on - strand, within Ac3H11_5001 at 171.914 kb on - strand, within Ac3H11_5001 at 172.081 kb on + strand, within Ac3H11_5001 at 172.081 kb on + strand, within Ac3H11_5001 at 172.082 kb on - strand, within Ac3H11_5001 at 172.082 kb on - strand, within Ac3H11_5001 at 172.082 kb on - strand, within Ac3H11_5001 at 172.082 kb on - strand, within Ac3H11_5001 at 172.082 kb on - strand, within Ac3H11_5001 at 172.082 kb on - strand, within Ac3H11_5001 at 172.082 kb on - strand, within Ac3H11_5001 at 172.183 kb on + strand at 172.183 kb on + strand at 172.183 kb on + strand at 172.184 kb on - strand at 172.219 kb on - strand at 172.281 kb on + strand at 172.281 kb on + strand at 172.281 kb on + strand at 172.282 kb on - strand at 172.282 kb on - strand at 172.282 kb on - strand at 172.296 kb on + strand at 172.317 kb on + strand at 172.317 kb on + strand at 172.317 kb on + strand at 172.318 kb on - strand at 172.318 kb on - strand at 172.485 kb on + strand, within Ac3H11_5002 at 172.486 kb on - strand, within Ac3H11_5002 at 172.500 kb on + strand, within Ac3H11_5002 at 172.776 kb on + strand, within Ac3H11_5002 at 172.777 kb on - strand, within Ac3H11_5002 at 172.777 kb on - strand, within Ac3H11_5002 at 172.920 kb on + strand, within Ac3H11_5002 at 172.920 kb on + strand, within Ac3H11_5002 at 172.920 kb on + strand, within Ac3H11_5002 at 172.921 kb on - strand, within Ac3H11_5002 at 173.058 kb on + strand, within Ac3H11_5002 at 173.058 kb on + strand, within Ac3H11_5002 at 173.058 kb on + strand, within Ac3H11_5002 at 173.058 kb on + strand, within Ac3H11_5002 at 173.059 kb on - strand, within Ac3H11_5002 at 173.059 kb on - strand, within Ac3H11_5002 at 173.059 kb on - strand, within Ac3H11_5002 at 173.059 kb on - strand, within Ac3H11_5002 at 173.059 kb on - strand, within Ac3H11_5002 at 173.059 kb on - strand, within Ac3H11_5002 at 173.059 kb on - strand, within Ac3H11_5002 at 173.394 kb on + strand, within Ac3H11_5002 at 173.394 kb on + strand, within Ac3H11_5002 at 173.395 kb on - strand, within Ac3H11_5002 at 173.395 kb on - strand, within Ac3H11_5002 at 173.658 kb on + strand, within Ac3H11_5002 at 173.658 kb on + strand, within Ac3H11_5002 at 173.658 kb on + strand, within Ac3H11_5002 at 173.658 kb on + strand, within Ac3H11_5002 at 173.658 kb on + strand, within Ac3H11_5002 at 173.658 kb on + strand, within Ac3H11_5002 at 173.659 kb on - strand, within Ac3H11_5002 at 173.659 kb on - strand, within Ac3H11_5002 at 173.659 kb on - strand, within Ac3H11_5002 at 173.659 kb on - strand, within Ac3H11_5002 at 173.659 kb on - strand, within Ac3H11_5002 at 173.805 kb on + strand, within Ac3H11_5002 at 173.805 kb on + strand, within Ac3H11_5002 at 173.805 kb on + strand, within Ac3H11_5002 at 173.805 kb on + strand, within Ac3H11_5002 at 173.805 kb on + strand, within Ac3H11_5002 at 173.806 kb on - strand, within Ac3H11_5002 at 173.806 kb on - strand, within Ac3H11_5002 at 173.806 kb on - strand, within Ac3H11_5002 at 173.806 kb on - strand, within Ac3H11_5002 at 173.806 kb on - strand, within Ac3H11_5002 at 173.835 kb on + strand, within Ac3H11_5002 at 173.836 kb on - strand, within Ac3H11_5002 at 173.836 kb on - strand, within Ac3H11_5002 at 173.836 kb on - strand, within Ac3H11_5002 at 173.994 kb on + strand at 173.995 kb on - strand at 173.995 kb on - strand at 173.995 kb on - strand at 174.566 kb on + strand, within Ac3H11_5003 at 174.566 kb on + strand, within Ac3H11_5003 at 174.566 kb on + strand, within Ac3H11_5003 at 174.566 kb on + strand, within Ac3H11_5003 at 174.567 kb on - strand, within Ac3H11_5003 at 174.567 kb on - strand at 174.567 kb on - strand, within Ac3H11_5003 at 174.567 kb on - strand, within Ac3H11_5003 at 174.567 kb on - strand, within Ac3H11_5003 at 174.567 kb on - strand, within Ac3H11_5003 at 174.567 kb on - strand, within Ac3H11_5003 at 174.670 kb on + strand at 174.670 kb on + strand at 174.705 kb on + strand at 174.705 kb on + strand at 174.705 kb on + strand at 174.706 kb on - strand at 174.723 kb on + strand at 174.724 kb on - strand at 174.724 kb on - strand at 174.804 kb on + strand at 174.804 kb on + strand at 174.804 kb on + strand at 174.804 kb on + strand at 174.804 kb on + strand at 174.804 kb on + strand at 174.805 kb on - strand at 174.805 kb on - strand at 174.805 kb on - strand at 174.805 kb on - strand at 174.805 kb on - strand at 174.846 kb on + strand at 174.846 kb on + strand at 174.846 kb on + strand at 174.846 kb on + strand at 174.846 kb on + strand at 174.846 kb on + strand at 174.846 kb on + strand at 174.847 kb on - strand at 174.847 kb on - strand at 174.847 kb on - strand at 174.847 kb on - strand at 174.847 kb on - strand at 174.931 kb on - strand, within Ac3H11_5004 at 175.060 kb on - strand, within Ac3H11_5004
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 171,400 + +0.5 171,400 + +0.1 171,400 + +1.2 171,400 + +0.6 171,400 + +0.7 171,400 + -0.2 171,401 - +0.8 171,401 - +0.1 171,622 + +1.5 171,622 + +0.4 171,622 + -0.3 171,622 + +0.1 171,622 + +0.8 171,623 - +0.0 171,623 - -1.0 171,634 + +0.3 171,664 - -0.1 171,677 + -0.5 171,678 - +0.7 171,844 + +0.1 171,844 + -0.5 171,845 - -1.1 171,845 - +0.1 171,845 - +0.2 171,845 - +0.4 171,913 + Ac3H11_5001 0.20 -0.3 171,913 + Ac3H11_5001 0.20 -0.8 171,913 + Ac3H11_5001 0.20 -0.7 171,913 + Ac3H11_5001 0.20 +0.0 171,914 - Ac3H11_5001 0.21 -1.1 171,914 - Ac3H11_5001 0.21 -0.4 171,914 - Ac3H11_5001 0.21 -0.2 171,914 - Ac3H11_5001 0.21 -0.8 171,914 - Ac3H11_5001 0.21 +0.9 172,081 + Ac3H11_5001 0.66 +0.9 172,081 + Ac3H11_5001 0.66 +0.1 172,082 - Ac3H11_5001 0.67 -0.6 172,082 - Ac3H11_5001 0.67 +0.0 172,082 - Ac3H11_5001 0.67 +0.4 172,082 - Ac3H11_5001 0.67 +0.1 172,082 - Ac3H11_5001 0.67 -0.4 172,082 - Ac3H11_5001 0.67 -0.4 172,082 - Ac3H11_5001 0.67 +1.1 172,183 + -0.8 172,183 + -0.8 172,183 + -0.3 172,184 - +0.0 172,219 - -0.1 172,281 + +0.3 172,281 + +0.3 172,281 + -0.1 172,282 - -0.4 172,282 - -0.3 172,282 - -0.1 172,296 + +0.6 172,317 + +0.9 172,317 + -0.4 172,317 + -0.9 172,318 - +0.4 172,318 - -0.4 172,485 + Ac3H11_5002 0.14 +1.2 172,486 - Ac3H11_5002 0.14 +0.9 172,500 + Ac3H11_5002 0.15 -0.9 172,776 + Ac3H11_5002 0.29 +0.7 172,777 - Ac3H11_5002 0.29 +1.3 172,777 - Ac3H11_5002 0.29 -0.5 172,920 + Ac3H11_5002 0.37 -0.1 172,920 + Ac3H11_5002 0.37 -1.2 172,920 + Ac3H11_5002 0.37 +0.7 172,921 - Ac3H11_5002 0.37 +0.1 173,058 + Ac3H11_5002 0.44 +0.6 173,058 + Ac3H11_5002 0.44 +0.5 173,058 + Ac3H11_5002 0.44 +0.1 173,058 + Ac3H11_5002 0.44 -0.5 173,059 - Ac3H11_5002 0.44 +0.3 173,059 - Ac3H11_5002 0.44 -1.1 173,059 - Ac3H11_5002 0.44 -0.5 173,059 - Ac3H11_5002 0.44 +0.9 173,059 - Ac3H11_5002 0.44 +0.8 173,059 - Ac3H11_5002 0.44 -1.3 173,059 - Ac3H11_5002 0.44 +0.7 173,394 + Ac3H11_5002 0.61 +0.8 173,394 + Ac3H11_5002 0.61 -0.7 173,395 - Ac3H11_5002 0.61 +0.7 173,395 - Ac3H11_5002 0.61 -0.1 173,658 + Ac3H11_5002 0.75 -0.6 173,658 + Ac3H11_5002 0.75 -0.6 173,658 + Ac3H11_5002 0.75 +0.2 173,658 + Ac3H11_5002 0.75 +1.2 173,658 + Ac3H11_5002 0.75 +2.9 173,658 + Ac3H11_5002 0.75 +0.1 173,659 - Ac3H11_5002 0.75 -0.1 173,659 - Ac3H11_5002 0.75 +0.8 173,659 - Ac3H11_5002 0.75 -0.2 173,659 - Ac3H11_5002 0.75 -0.1 173,659 - Ac3H11_5002 0.75 -0.2 173,805 + Ac3H11_5002 0.82 -0.1 173,805 + Ac3H11_5002 0.82 +0.1 173,805 + Ac3H11_5002 0.82 +1.2 173,805 + Ac3H11_5002 0.82 -0.5 173,805 + Ac3H11_5002 0.82 +0.5 173,806 - Ac3H11_5002 0.83 -0.7 173,806 - Ac3H11_5002 0.83 -0.4 173,806 - Ac3H11_5002 0.83 -0.1 173,806 - Ac3H11_5002 0.83 -0.0 173,806 - Ac3H11_5002 0.83 -0.1 173,835 + Ac3H11_5002 0.84 +0.0 173,836 - Ac3H11_5002 0.84 -0.5 173,836 - Ac3H11_5002 0.84 -0.7 173,836 - Ac3H11_5002 0.84 +0.9 173,994 + -0.3 173,995 - -2.2 173,995 - -0.0 173,995 - -0.3 174,566 + Ac3H11_5003 0.82 -1.0 174,566 + Ac3H11_5003 0.82 -0.4 174,566 + Ac3H11_5003 0.82 +0.4 174,566 + Ac3H11_5003 0.82 -0.1 174,567 - Ac3H11_5003 0.82 -0.4 174,567 - +1.5 174,567 - Ac3H11_5003 0.82 -0.7 174,567 - Ac3H11_5003 0.82 -1.1 174,567 - Ac3H11_5003 0.82 -0.4 174,567 - Ac3H11_5003 0.82 -0.9 174,567 - Ac3H11_5003 0.82 -0.1 174,670 + -0.1 174,670 + +0.1 174,705 + +0.1 174,705 + +0.1 174,705 + -0.1 174,706 - -0.8 174,723 + -0.1 174,724 - -0.1 174,724 - +0.1 174,804 + +0.8 174,804 + +0.2 174,804 + +0.7 174,804 + -0.4 174,804 + -1.2 174,804 + -0.2 174,805 - +0.1 174,805 - +0.9 174,805 - +0.2 174,805 - +0.5 174,805 - +0.2 174,846 + -1.6 174,846 + +1.8 174,846 + +0.4 174,846 + -0.0 174,846 + +0.2 174,846 + -0.4 174,846 + +0.8 174,847 - -0.2 174,847 - -0.1 174,847 - +0.4 174,847 - -1.0 174,847 - +0.3 174,931 - Ac3H11_5004 0.12 +0.5 175,060 - Ac3H11_5004 0.19 +0.6
Or see this region's nucleotide sequence