Experiment: m-Inositol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_04635 and AO353_04640 are separated by 89 nucleotides AO353_04640 and AO353_04645 are separated by 176 nucleotides
AO353_04635: AO353_04635 - FAD-dependent oxidoreductase, at 1,000,818 to 1,001,945
_04635
AO353_04640: AO353_04640 - hypothetical protein, at 1,002,035 to 1,002,661
_04640
AO353_04645: AO353_04645 - ABC transporter substrate-binding protein, at 1,002,838 to 1,003,614
_04645
Position (kb)
1002
1003 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1001.124 kb on + strand, within AO353_04635 at 1001.125 kb on - strand, within AO353_04635 at 1001.125 kb on - strand, within AO353_04635 at 1001.125 kb on - strand, within AO353_04635 at 1001.229 kb on + strand, within AO353_04635 at 1001.229 kb on + strand, within AO353_04635 at 1001.229 kb on + strand, within AO353_04635 at 1001.229 kb on + strand, within AO353_04635 at 1001.229 kb on + strand, within AO353_04635 at 1001.230 kb on - strand, within AO353_04635 at 1001.230 kb on - strand, within AO353_04635 at 1001.230 kb on - strand, within AO353_04635 at 1001.230 kb on - strand, within AO353_04635 at 1001.230 kb on - strand, within AO353_04635 at 1001.230 kb on - strand, within AO353_04635 at 1001.230 kb on - strand, within AO353_04635 at 1001.278 kb on - strand, within AO353_04635 at 1001.278 kb on - strand, within AO353_04635 at 1001.565 kb on + strand, within AO353_04635 at 1001.565 kb on + strand, within AO353_04635 at 1001.565 kb on + strand, within AO353_04635 at 1001.565 kb on + strand, within AO353_04635 at 1001.565 kb on + strand, within AO353_04635 at 1001.565 kb on + strand, within AO353_04635 at 1001.565 kb on + strand, within AO353_04635 at 1001.565 kb on + strand, within AO353_04635 at 1001.565 kb on + strand, within AO353_04635 at 1001.565 kb on + strand, within AO353_04635 at 1001.566 kb on - strand, within AO353_04635 at 1001.566 kb on - strand, within AO353_04635 at 1001.566 kb on - strand, within AO353_04635 at 1001.566 kb on - strand, within AO353_04635 at 1001.566 kb on - strand, within AO353_04635 at 1001.566 kb on - strand, within AO353_04635 at 1001.566 kb on - strand, within AO353_04635 at 1001.566 kb on - strand, within AO353_04635 at 1001.566 kb on - strand, within AO353_04635 at 1001.566 kb on - strand, within AO353_04635 at 1001.670 kb on + strand, within AO353_04635 at 1001.670 kb on + strand, within AO353_04635 at 1001.670 kb on + strand, within AO353_04635 at 1001.731 kb on + strand, within AO353_04635 at 1001.732 kb on - strand, within AO353_04635 at 1001.782 kb on + strand, within AO353_04635 at 1001.783 kb on - strand, within AO353_04635 at 1002.068 kb on - strand at 1002.071 kb on + strand at 1002.158 kb on + strand, within AO353_04640 at 1002.158 kb on + strand, within AO353_04640 at 1002.158 kb on + strand, within AO353_04640 at 1002.158 kb on + strand, within AO353_04640 at 1002.159 kb on - strand, within AO353_04640 at 1002.159 kb on - strand, within AO353_04640 at 1002.254 kb on + strand, within AO353_04640 at 1002.254 kb on + strand, within AO353_04640 at 1002.255 kb on - strand, within AO353_04640 at 1002.355 kb on + strand, within AO353_04640 at 1002.355 kb on + strand, within AO353_04640 at 1002.356 kb on - strand, within AO353_04640 at 1002.356 kb on - strand, within AO353_04640 at 1002.356 kb on - strand, within AO353_04640 at 1002.356 kb on - strand at 1002.356 kb on - strand, within AO353_04640 at 1002.409 kb on + strand, within AO353_04640 at 1002.410 kb on - strand, within AO353_04640 at 1002.478 kb on + strand, within AO353_04640 at 1002.478 kb on + strand, within AO353_04640 at 1002.478 kb on + strand, within AO353_04640 at 1002.478 kb on + strand, within AO353_04640 at 1002.478 kb on + strand, within AO353_04640 at 1002.478 kb on + strand, within AO353_04640 at 1002.478 kb on + strand, within AO353_04640 at 1002.478 kb on + strand, within AO353_04640 at 1002.479 kb on - strand, within AO353_04640 at 1002.479 kb on - strand, within AO353_04640 at 1002.479 kb on - strand, within AO353_04640 at 1002.479 kb on - strand, within AO353_04640 at 1002.479 kb on - strand, within AO353_04640 at 1002.479 kb on - strand, within AO353_04640 at 1002.479 kb on - strand, within AO353_04640 at 1002.479 kb on - strand, within AO353_04640 at 1002.479 kb on - strand, within AO353_04640 at 1002.511 kb on + strand, within AO353_04640 at 1002.511 kb on + strand, within AO353_04640 at 1002.511 kb on + strand, within AO353_04640 at 1002.511 kb on + strand, within AO353_04640 at 1002.512 kb on - strand, within AO353_04640 at 1002.512 kb on - strand, within AO353_04640 at 1002.512 kb on - strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.593 kb on + strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.594 kb on - strand, within AO353_04640 at 1002.597 kb on + strand, within AO353_04640 at 1002.597 kb on + strand, within AO353_04640 at 1002.597 kb on + strand, within AO353_04640 at 1002.597 kb on + strand, within AO353_04640 at 1002.597 kb on + strand, within AO353_04640 at 1002.597 kb on + strand, within AO353_04640 at 1002.597 kb on + strand, within AO353_04640 at 1002.597 kb on + strand, within AO353_04640 at 1002.598 kb on - strand, within AO353_04640 at 1002.598 kb on - strand, within AO353_04640 at 1002.598 kb on - strand, within AO353_04640 at 1002.598 kb on - strand, within AO353_04640 at 1002.632 kb on + strand at 1002.633 kb on - strand at 1002.633 kb on - strand at 1002.633 kb on - strand at 1002.659 kb on + strand at 1002.660 kb on - strand at 1002.667 kb on + strand at 1002.667 kb on + strand at 1002.667 kb on + strand at 1002.668 kb on - strand at 1002.668 kb on - strand at 1002.682 kb on - strand at 1002.768 kb on + strand at 1002.768 kb on + strand at 1002.768 kb on + strand at 1002.768 kb on + strand at 1002.769 kb on - strand at 1002.848 kb on + strand at 1002.848 kb on + strand at 1002.848 kb on + strand at 1002.848 kb on + strand at 1002.848 kb on + strand at 1002.849 kb on - strand at 1002.852 kb on + strand at 1002.852 kb on + strand at 1002.853 kb on - strand at 1002.853 kb on - strand at 1002.866 kb on + strand at 1002.866 kb on + strand at 1002.866 kb on + strand at 1002.866 kb on + strand at 1002.867 kb on - strand at 1002.867 kb on - strand at 1002.867 kb on - strand at 1002.986 kb on + strand, within AO353_04645 at 1002.986 kb on + strand, within AO353_04645 at 1002.987 kb on - strand, within AO353_04645 at 1002.987 kb on - strand, within AO353_04645 at 1002.987 kb on - strand, within AO353_04645 at 1003.068 kb on + strand, within AO353_04645 at 1003.112 kb on + strand, within AO353_04645 at 1003.112 kb on + strand, within AO353_04645 at 1003.112 kb on + strand, within AO353_04645 at 1003.112 kb on + strand, within AO353_04645 at 1003.112 kb on + strand, within AO353_04645 at 1003.112 kb on + strand, within AO353_04645 at 1003.113 kb on - strand, within AO353_04645 at 1003.113 kb on - strand, within AO353_04645 at 1003.113 kb on - strand, within AO353_04645 at 1003.113 kb on - strand, within AO353_04645 at 1003.113 kb on - strand, within AO353_04645 at 1003.113 kb on - strand, within AO353_04645 at 1003.113 kb on - strand, within AO353_04645 at 1003.113 kb on - strand, within AO353_04645 at 1003.113 kb on - strand, within AO353_04645 at 1003.113 kb on - strand, within AO353_04645 at 1003.133 kb on + strand, within AO353_04645 at 1003.289 kb on + strand, within AO353_04645 at 1003.289 kb on + strand, within AO353_04645 at 1003.289 kb on + strand, within AO353_04645 at 1003.289 kb on + strand, within AO353_04645 at 1003.289 kb on + strand, within AO353_04645 at 1003.289 kb on + strand, within AO353_04645 at 1003.289 kb on + strand, within AO353_04645 at 1003.289 kb on + strand, within AO353_04645 at 1003.289 kb on + strand, within AO353_04645 at 1003.290 kb on - strand, within AO353_04645 at 1003.290 kb on - strand, within AO353_04645 at 1003.290 kb on - strand, within AO353_04645 at 1003.290 kb on - strand, within AO353_04645 at 1003.290 kb on - strand, within AO353_04645 at 1003.290 kb on - strand, within AO353_04645 at 1003.290 kb on - strand, within AO353_04645 at 1003.292 kb on + strand, within AO353_04645 at 1003.292 kb on + strand, within AO353_04645 at 1003.292 kb on + strand, within AO353_04645 at 1003.292 kb on + strand, within AO353_04645 at 1003.292 kb on + strand, within AO353_04645 at 1003.292 kb on + strand, within AO353_04645 at 1003.292 kb on + strand, within AO353_04645 at 1003.292 kb on + strand, within AO353_04645 at 1003.292 kb on + strand, within AO353_04645 at 1003.293 kb on - strand, within AO353_04645 at 1003.293 kb on - strand, within AO353_04645 at 1003.293 kb on - strand, within AO353_04645 at 1003.293 kb on - strand, within AO353_04645 at 1003.293 kb on - strand, within AO353_04645 at 1003.293 kb on - strand, within AO353_04645 at 1003.321 kb on - strand, within AO353_04645 at 1003.321 kb on - strand, within AO353_04645 at 1003.461 kb on - strand, within AO353_04645
Per-strain Table
Position Strand Gene LocusTag Fraction m-Inositol (C) remove 1,001,124 + AO353_04635 0.27 +0.2 1,001,125 - AO353_04635 0.27 +1.8 1,001,125 - AO353_04635 0.27 -1.7 1,001,125 - AO353_04635 0.27 -0.3 1,001,229 + AO353_04635 0.36 +0.5 1,001,229 + AO353_04635 0.36 -1.4 1,001,229 + AO353_04635 0.36 +1.2 1,001,229 + AO353_04635 0.36 +0.5 1,001,229 + AO353_04635 0.36 +0.7 1,001,230 - AO353_04635 0.37 +0.2 1,001,230 - AO353_04635 0.37 +0.0 1,001,230 - AO353_04635 0.37 -0.7 1,001,230 - AO353_04635 0.37 -1.2 1,001,230 - AO353_04635 0.37 -0.5 1,001,230 - AO353_04635 0.37 +1.5 1,001,230 - AO353_04635 0.37 +0.2 1,001,278 - AO353_04635 0.41 +0.7 1,001,278 - AO353_04635 0.41 +1.3 1,001,565 + AO353_04635 0.66 +0.0 1,001,565 + AO353_04635 0.66 +0.3 1,001,565 + AO353_04635 0.66 -0.0 1,001,565 + AO353_04635 0.66 +0.3 1,001,565 + AO353_04635 0.66 -1.0 1,001,565 + AO353_04635 0.66 -2.0 1,001,565 + AO353_04635 0.66 +0.1 1,001,565 + AO353_04635 0.66 +2.1 1,001,565 + AO353_04635 0.66 -0.8 1,001,565 + AO353_04635 0.66 -1.4 1,001,566 - AO353_04635 0.66 -1.0 1,001,566 - AO353_04635 0.66 +0.2 1,001,566 - AO353_04635 0.66 -0.7 1,001,566 - AO353_04635 0.66 +0.1 1,001,566 - AO353_04635 0.66 +1.1 1,001,566 - AO353_04635 0.66 -0.2 1,001,566 - AO353_04635 0.66 -0.4 1,001,566 - AO353_04635 0.66 +1.3 1,001,566 - AO353_04635 0.66 -1.3 1,001,566 - AO353_04635 0.66 +0.5 1,001,670 + AO353_04635 0.76 +0.1 1,001,670 + AO353_04635 0.76 -0.2 1,001,670 + AO353_04635 0.76 +0.8 1,001,731 + AO353_04635 0.81 +1.3 1,001,732 - AO353_04635 0.81 -0.2 1,001,782 + AO353_04635 0.85 -0.2 1,001,783 - AO353_04635 0.86 -0.1 1,002,068 - -0.2 1,002,071 + -1.8 1,002,158 + AO353_04640 0.20 -0.5 1,002,158 + AO353_04640 0.20 -0.1 1,002,158 + AO353_04640 0.20 -0.7 1,002,158 + AO353_04640 0.20 -0.0 1,002,159 - AO353_04640 0.20 +0.8 1,002,159 - AO353_04640 0.20 -0.7 1,002,254 + AO353_04640 0.35 -0.7 1,002,254 + AO353_04640 0.35 -1.3 1,002,255 - AO353_04640 0.35 -2.5 1,002,355 + AO353_04640 0.51 +0.2 1,002,355 + AO353_04640 0.51 +1.8 1,002,356 - AO353_04640 0.51 -1.5 1,002,356 - AO353_04640 0.51 +1.4 1,002,356 - AO353_04640 0.51 -0.6 1,002,356 - -0.8 1,002,356 - AO353_04640 0.51 -0.5 1,002,409 + AO353_04640 0.60 +1.6 1,002,410 - AO353_04640 0.60 -2.1 1,002,478 + AO353_04640 0.71 -0.4 1,002,478 + AO353_04640 0.71 +0.8 1,002,478 + AO353_04640 0.71 +0.4 1,002,478 + AO353_04640 0.71 +0.2 1,002,478 + AO353_04640 0.71 -0.5 1,002,478 + AO353_04640 0.71 -0.7 1,002,478 + AO353_04640 0.71 +2.2 1,002,478 + AO353_04640 0.71 -0.8 1,002,479 - AO353_04640 0.71 -0.5 1,002,479 - AO353_04640 0.71 -0.5 1,002,479 - AO353_04640 0.71 -1.9 1,002,479 - AO353_04640 0.71 +1.1 1,002,479 - AO353_04640 0.71 +0.8 1,002,479 - AO353_04640 0.71 -0.7 1,002,479 - AO353_04640 0.71 -0.5 1,002,479 - AO353_04640 0.71 -0.1 1,002,479 - AO353_04640 0.71 -1.7 1,002,511 + AO353_04640 0.76 +0.7 1,002,511 + AO353_04640 0.76 +0.2 1,002,511 + AO353_04640 0.76 +1.8 1,002,511 + AO353_04640 0.76 +0.0 1,002,512 - AO353_04640 0.76 +0.4 1,002,512 - AO353_04640 0.76 +0.2 1,002,512 - AO353_04640 0.76 -1.5 1,002,593 + AO353_04640 0.89 +0.3 1,002,593 + AO353_04640 0.89 -0.8 1,002,593 + AO353_04640 0.89 -0.4 1,002,593 + AO353_04640 0.89 -2.3 1,002,593 + AO353_04640 0.89 -0.4 1,002,593 + AO353_04640 0.89 +1.2 1,002,593 + AO353_04640 0.89 -0.8 1,002,593 + AO353_04640 0.89 -1.5 1,002,593 + AO353_04640 0.89 -0.6 1,002,593 + AO353_04640 0.89 -0.5 1,002,593 + AO353_04640 0.89 -1.2 1,002,593 + AO353_04640 0.89 +0.6 1,002,593 + AO353_04640 0.89 +2.6 1,002,593 + AO353_04640 0.89 +0.5 1,002,593 + AO353_04640 0.89 +0.9 1,002,593 + AO353_04640 0.89 -1.0 1,002,593 + AO353_04640 0.89 -0.2 1,002,593 + AO353_04640 0.89 -0.6 1,002,593 + AO353_04640 0.89 -1.7 1,002,593 + AO353_04640 0.89 -1.7 1,002,594 - AO353_04640 0.89 +0.9 1,002,594 - AO353_04640 0.89 +0.1 1,002,594 - AO353_04640 0.89 -2.1 1,002,594 - AO353_04640 0.89 -2.5 1,002,594 - AO353_04640 0.89 -0.2 1,002,594 - AO353_04640 0.89 +0.3 1,002,594 - AO353_04640 0.89 -0.3 1,002,594 - AO353_04640 0.89 -0.3 1,002,594 - AO353_04640 0.89 -1.0 1,002,594 - AO353_04640 0.89 +0.6 1,002,594 - AO353_04640 0.89 +0.2 1,002,594 - AO353_04640 0.89 -0.9 1,002,594 - AO353_04640 0.89 +0.7 1,002,594 - AO353_04640 0.89 -2.7 1,002,594 - AO353_04640 0.89 -0.4 1,002,594 - AO353_04640 0.89 -1.0 1,002,594 - AO353_04640 0.89 -0.2 1,002,594 - AO353_04640 0.89 -1.1 1,002,594 - AO353_04640 0.89 -0.8 1,002,594 - AO353_04640 0.89 -0.3 1,002,594 - AO353_04640 0.89 -0.8 1,002,594 - AO353_04640 0.89 +0.2 1,002,597 + AO353_04640 0.90 -0.0 1,002,597 + AO353_04640 0.90 -1.1 1,002,597 + AO353_04640 0.90 -0.7 1,002,597 + AO353_04640 0.90 -0.7 1,002,597 + AO353_04640 0.90 -1.2 1,002,597 + AO353_04640 0.90 -0.1 1,002,597 + AO353_04640 0.90 -0.7 1,002,597 + AO353_04640 0.90 -0.5 1,002,598 - AO353_04640 0.90 -1.4 1,002,598 - AO353_04640 0.90 -0.0 1,002,598 - AO353_04640 0.90 -0.5 1,002,598 - AO353_04640 0.90 +0.2 1,002,632 + -0.2 1,002,633 - +1.4 1,002,633 - -2.3 1,002,633 - -0.4 1,002,659 + -0.0 1,002,660 - -1.5 1,002,667 + +0.5 1,002,667 + -0.3 1,002,667 + +0.2 1,002,668 - -0.9 1,002,668 - -1.4 1,002,682 - +0.2 1,002,768 + +1.8 1,002,768 + -1.0 1,002,768 + +0.1 1,002,768 + +0.7 1,002,769 - -0.2 1,002,848 + -0.5 1,002,848 + +0.0 1,002,848 + -1.0 1,002,848 + -0.8 1,002,848 + -1.0 1,002,849 - -0.8 1,002,852 + -0.4 1,002,852 + +0.5 1,002,853 - -1.5 1,002,853 - +1.6 1,002,866 + -0.9 1,002,866 + -1.4 1,002,866 + +1.4 1,002,866 + +1.2 1,002,867 - +1.2 1,002,867 - -0.0 1,002,867 - -0.2 1,002,986 + AO353_04645 0.19 -0.5 1,002,986 + AO353_04645 0.19 +1.5 1,002,987 - AO353_04645 0.19 -1.5 1,002,987 - AO353_04645 0.19 +0.6 1,002,987 - AO353_04645 0.19 +0.2 1,003,068 + AO353_04645 0.30 +1.3 1,003,112 + AO353_04645 0.35 +0.9 1,003,112 + AO353_04645 0.35 -0.2 1,003,112 + AO353_04645 0.35 -0.3 1,003,112 + AO353_04645 0.35 -0.5 1,003,112 + AO353_04645 0.35 +0.5 1,003,112 + AO353_04645 0.35 -0.6 1,003,113 - AO353_04645 0.35 -0.9 1,003,113 - AO353_04645 0.35 -0.4 1,003,113 - AO353_04645 0.35 +0.8 1,003,113 - AO353_04645 0.35 -0.5 1,003,113 - AO353_04645 0.35 -1.0 1,003,113 - AO353_04645 0.35 -0.8 1,003,113 - AO353_04645 0.35 +0.5 1,003,113 - AO353_04645 0.35 +1.2 1,003,113 - AO353_04645 0.35 +0.2 1,003,113 - AO353_04645 0.35 -0.0 1,003,133 + AO353_04645 0.38 -0.0 1,003,289 + AO353_04645 0.58 -1.3 1,003,289 + AO353_04645 0.58 -1.0 1,003,289 + AO353_04645 0.58 -0.2 1,003,289 + AO353_04645 0.58 -0.7 1,003,289 + AO353_04645 0.58 +0.8 1,003,289 + AO353_04645 0.58 -0.3 1,003,289 + AO353_04645 0.58 -0.0 1,003,289 + AO353_04645 0.58 -1.4 1,003,289 + AO353_04645 0.58 -1.0 1,003,290 - AO353_04645 0.58 -0.3 1,003,290 - AO353_04645 0.58 -0.7 1,003,290 - AO353_04645 0.58 -1.2 1,003,290 - AO353_04645 0.58 +0.8 1,003,290 - AO353_04645 0.58 +1.1 1,003,290 - AO353_04645 0.58 +0.1 1,003,290 - AO353_04645 0.58 -0.3 1,003,292 + AO353_04645 0.58 +1.0 1,003,292 + AO353_04645 0.58 +1.6 1,003,292 + AO353_04645 0.58 -0.5 1,003,292 + AO353_04645 0.58 -0.8 1,003,292 + AO353_04645 0.58 +0.1 1,003,292 + AO353_04645 0.58 -0.8 1,003,292 + AO353_04645 0.58 -0.4 1,003,292 + AO353_04645 0.58 -0.3 1,003,292 + AO353_04645 0.58 +0.4 1,003,293 - AO353_04645 0.59 +0.9 1,003,293 - AO353_04645 0.59 +1.5 1,003,293 - AO353_04645 0.59 +2.1 1,003,293 - AO353_04645 0.59 -0.5 1,003,293 - AO353_04645 0.59 -0.5 1,003,293 - AO353_04645 0.59 -1.0 1,003,321 - AO353_04645 0.62 -0.5 1,003,321 - AO353_04645 0.62 -0.7 1,003,461 - AO353_04645 0.80 +0.4
Or see this region's nucleotide sequence