Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3215 and Shewana3_3216 overlap by 4 nucleotides Shewana3_3216 and obgE are separated by 27 nucleotides obgE and rpmA are separated by 212 nucleotides rpmA and rplU are separated by 14 nucleotides
Shewana3_3215: Shewana3_3215 - hypothetical protein (RefSeq), at 3,859,343 to 3,859,816
_3215
Shewana3_3216: Shewana3_3216 - hypothetical protein (RefSeq), at 3,859,813 to 3,860,574
_3216
Shewana3_3217: obgE - GTPase ObgE (RefSeq), at 3,860,602 to 3,861,768
obgE
Shewana3_3218: rpmA - 50S ribosomal protein L27 (RefSeq), at 3,861,981 to 3,862,235
rpmA
Shewana3_3219: rplU - 50S ribosomal protein L21 (RefSeq), at 3,862,250 to 3,862,561
rplU
Position (kb) 3860
3861
3862 Strain fitness (log2 ratio) -1
0
1 at 3859.937 kb on - strand, within Shewana3_3216 at 3860.205 kb on - strand, within Shewana3_3216 at 3860.409 kb on - strand, within Shewana3_3216 at 3860.463 kb on - strand, within Shewana3_3216 at 3860.539 kb on - strand at 3860.698 kb on - strand at 3860.728 kb on - strand, within obgE
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 3,859,937 - Shewana3_3216 0.16 +1.0 3,860,205 - Shewana3_3216 0.51 -0.8 3,860,409 - Shewana3_3216 0.78 -1.1 3,860,463 - Shewana3_3216 0.85 -0.9 3,860,539 - -1.1 3,860,698 - -1.1 3,860,728 - obgE Shewana3_3217 0.11 -0.8
Or see this region's nucleotide sequence