Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22065 and CA265_RS22070 are separated by 375 nucleotides CA265_RS22070 and CA265_RS22075 are separated by 519 nucleotides
CA265_RS22065: CA265_RS22065 - hypothetical protein, at 5,424,673 to 5,425,089
_RS22065
CA265_RS22070: CA265_RS22070 - hypothetical protein, at 5,425,465 to 5,425,662
_RS22070
CA265_RS22075: CA265_RS22075 - hypothetical protein, at 5,426,182 to 5,426,601
_RS22075
Position (kb)
5425
5426 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5424.499 kb on - strand at 5424.614 kb on + strand at 5424.614 kb on + strand at 5424.643 kb on + strand at 5424.644 kb on - strand at 5424.657 kb on + strand at 5424.666 kb on - strand at 5424.672 kb on + strand at 5424.673 kb on - strand at 5424.724 kb on - strand, within CA265_RS22065 at 5424.767 kb on + strand, within CA265_RS22065 at 5424.872 kb on + strand, within CA265_RS22065 at 5424.873 kb on - strand, within CA265_RS22065 at 5424.882 kb on - strand, within CA265_RS22065 at 5424.895 kb on - strand, within CA265_RS22065 at 5424.969 kb on - strand, within CA265_RS22065 at 5424.983 kb on - strand, within CA265_RS22065 at 5424.983 kb on - strand, within CA265_RS22065 at 5424.983 kb on - strand, within CA265_RS22065 at 5424.987 kb on - strand, within CA265_RS22065 at 5424.997 kb on - strand, within CA265_RS22065 at 5425.044 kb on - strand, within CA265_RS22065 at 5425.050 kb on - strand at 5425.050 kb on - strand at 5425.234 kb on + strand at 5425.235 kb on - strand at 5425.249 kb on - strand at 5425.292 kb on + strand at 5425.299 kb on - strand at 5425.331 kb on + strand at 5425.332 kb on - strand at 5425.494 kb on + strand, within CA265_RS22070 at 5425.496 kb on + strand, within CA265_RS22070 at 5425.505 kb on + strand, within CA265_RS22070 at 5425.546 kb on + strand, within CA265_RS22070 at 5425.549 kb on - strand, within CA265_RS22070 at 5425.560 kb on + strand, within CA265_RS22070 at 5425.631 kb on + strand, within CA265_RS22070 at 5425.631 kb on + strand, within CA265_RS22070 at 5425.633 kb on + strand, within CA265_RS22070 at 5425.633 kb on + strand, within CA265_RS22070 at 5425.661 kb on - strand at 5425.666 kb on + strand at 5425.666 kb on + strand at 5425.691 kb on - strand at 5425.699 kb on + strand at 5425.764 kb on + strand at 5425.785 kb on - strand at 5425.785 kb on - strand at 5425.805 kb on - strand at 5425.891 kb on + strand at 5425.892 kb on - strand at 5425.997 kb on + strand at 5425.998 kb on - strand at 5426.012 kb on + strand at 5426.026 kb on + strand at 5426.032 kb on - strand at 5426.079 kb on + strand at 5426.080 kb on - strand at 5426.108 kb on + strand at 5426.126 kb on - strand at 5426.126 kb on - strand at 5426.126 kb on - strand at 5426.128 kb on - strand at 5426.162 kb on + strand at 5426.193 kb on - strand at 5426.193 kb on - strand at 5426.268 kb on + strand, within CA265_RS22075 at 5426.280 kb on + strand, within CA265_RS22075 at 5426.281 kb on - strand, within CA265_RS22075 at 5426.325 kb on + strand, within CA265_RS22075 at 5426.326 kb on - strand, within CA265_RS22075 at 5426.326 kb on - strand, within CA265_RS22075 at 5426.326 kb on - strand, within CA265_RS22075 at 5426.326 kb on - strand, within CA265_RS22075 at 5426.327 kb on + strand, within CA265_RS22075 at 5426.416 kb on - strand, within CA265_RS22075 at 5426.424 kb on + strand, within CA265_RS22075 at 5426.460 kb on + strand, within CA265_RS22075 at 5426.460 kb on + strand, within CA265_RS22075 at 5426.461 kb on - strand, within CA265_RS22075 at 5426.540 kb on + strand at 5426.608 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 5,424,499 - +0.1 5,424,614 + -1.3 5,424,614 + -1.0 5,424,643 + +0.7 5,424,644 - -0.1 5,424,657 + +0.2 5,424,666 - -0.6 5,424,672 + -0.5 5,424,673 - -0.5 5,424,724 - CA265_RS22065 0.12 -0.5 5,424,767 + CA265_RS22065 0.23 -1.4 5,424,872 + CA265_RS22065 0.48 -1.5 5,424,873 - CA265_RS22065 0.48 -0.9 5,424,882 - CA265_RS22065 0.50 -2.5 5,424,895 - CA265_RS22065 0.53 -2.3 5,424,969 - CA265_RS22065 0.71 -0.0 5,424,983 - CA265_RS22065 0.74 -0.2 5,424,983 - CA265_RS22065 0.74 +0.0 5,424,983 - CA265_RS22065 0.74 +1.2 5,424,987 - CA265_RS22065 0.75 -0.5 5,424,997 - CA265_RS22065 0.78 -1.0 5,425,044 - CA265_RS22065 0.89 -1.6 5,425,050 - -1.1 5,425,050 - +0.7 5,425,234 + -0.1 5,425,235 - -0.4 5,425,249 - -0.2 5,425,292 + +0.1 5,425,299 - -0.1 5,425,331 + +1.4 5,425,332 - -0.3 5,425,494 + CA265_RS22070 0.15 +0.5 5,425,496 + CA265_RS22070 0.16 +0.1 5,425,505 + CA265_RS22070 0.20 +0.4 5,425,546 + CA265_RS22070 0.41 -0.9 5,425,549 - CA265_RS22070 0.42 +0.7 5,425,560 + CA265_RS22070 0.48 -0.7 5,425,631 + CA265_RS22070 0.84 -0.0 5,425,631 + CA265_RS22070 0.84 -0.2 5,425,633 + CA265_RS22070 0.85 -1.1 5,425,633 + CA265_RS22070 0.85 +0.1 5,425,661 - +0.5 5,425,666 + -2.0 5,425,666 + -0.3 5,425,691 - -0.5 5,425,699 + -0.6 5,425,764 + +2.9 5,425,785 - -0.8 5,425,785 - -0.6 5,425,805 - -0.2 5,425,891 + -1.4 5,425,892 - -0.3 5,425,997 + +0.6 5,425,998 - +0.6 5,426,012 + +0.1 5,426,026 + +0.2 5,426,032 - -0.5 5,426,079 + +0.3 5,426,080 - +0.5 5,426,108 + -0.1 5,426,126 - +1.4 5,426,126 - +0.0 5,426,126 - +0.3 5,426,128 - -0.5 5,426,162 + +1.1 5,426,193 - +0.6 5,426,193 - +1.1 5,426,268 + CA265_RS22075 0.20 +0.9 5,426,280 + CA265_RS22075 0.23 +0.7 5,426,281 - CA265_RS22075 0.24 +0.9 5,426,325 + CA265_RS22075 0.34 +0.7 5,426,326 - CA265_RS22075 0.34 -0.5 5,426,326 - CA265_RS22075 0.34 +1.1 5,426,326 - CA265_RS22075 0.34 +0.9 5,426,326 - CA265_RS22075 0.34 -0.9 5,426,327 + CA265_RS22075 0.35 +0.1 5,426,416 - CA265_RS22075 0.56 +0.4 5,426,424 + CA265_RS22075 0.58 -1.0 5,426,460 + CA265_RS22075 0.66 +1.2 5,426,460 + CA265_RS22075 0.66 -1.4 5,426,461 - CA265_RS22075 0.66 +0.3 5,426,540 + +1.7 5,426,608 + +0.6
Or see this region's nucleotide sequence