Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15205 and CA265_RS15210 are separated by 52 nucleotides CA265_RS15210 and CA265_RS15215 are separated by 134 nucleotides
CA265_RS15205: CA265_RS15205 - aspartate aminotransferase family protein, at 3,825,768 to 3,826,904
_RS15205
CA265_RS15210: CA265_RS15210 - DNA-binding response regulator, at 3,826,957 to 3,827,634
_RS15210
CA265_RS15215: CA265_RS15215 - two-component sensor histidine kinase, at 3,827,769 to 3,829,073
_RS15215
Position (kb)
3826
3827
3828 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3826.092 kb on - strand, within CA265_RS15205 at 3826.110 kb on + strand, within CA265_RS15205 at 3826.118 kb on + strand, within CA265_RS15205 at 3826.122 kb on - strand, within CA265_RS15205 at 3826.193 kb on - strand, within CA265_RS15205 at 3826.234 kb on + strand, within CA265_RS15205 at 3826.271 kb on - strand, within CA265_RS15205 at 3826.289 kb on - strand, within CA265_RS15205 at 3826.308 kb on + strand, within CA265_RS15205 at 3826.309 kb on - strand, within CA265_RS15205 at 3826.309 kb on - strand, within CA265_RS15205 at 3826.311 kb on - strand, within CA265_RS15205 at 3826.426 kb on + strand, within CA265_RS15205 at 3826.521 kb on - strand, within CA265_RS15205 at 3826.591 kb on + strand, within CA265_RS15205 at 3826.591 kb on + strand, within CA265_RS15205 at 3826.592 kb on - strand, within CA265_RS15205 at 3826.771 kb on + strand, within CA265_RS15205 at 3826.772 kb on - strand, within CA265_RS15205 at 3826.830 kb on - strand at 3826.879 kb on + strand at 3826.964 kb on + strand at 3826.964 kb on + strand at 3826.965 kb on - strand at 3827.020 kb on - strand at 3827.031 kb on + strand, within CA265_RS15210 at 3827.032 kb on - strand, within CA265_RS15210 at 3827.076 kb on - strand, within CA265_RS15210 at 3827.082 kb on - strand, within CA265_RS15210 at 3827.153 kb on + strand, within CA265_RS15210 at 3827.223 kb on + strand, within CA265_RS15210 at 3827.251 kb on + strand, within CA265_RS15210 at 3827.292 kb on + strand, within CA265_RS15210 at 3827.349 kb on + strand, within CA265_RS15210 at 3827.508 kb on - strand, within CA265_RS15210 at 3827.625 kb on + strand at 3827.687 kb on + strand at 3827.705 kb on - strand at 3827.746 kb on - strand at 3827.797 kb on - strand at 3827.811 kb on - strand at 3827.858 kb on - strand at 3827.953 kb on + strand, within CA265_RS15215 at 3827.984 kb on + strand, within CA265_RS15215 at 3828.003 kb on - strand, within CA265_RS15215 at 3828.041 kb on + strand, within CA265_RS15215 at 3828.052 kb on + strand, within CA265_RS15215 at 3828.053 kb on - strand, within CA265_RS15215 at 3828.083 kb on + strand, within CA265_RS15215 at 3828.198 kb on + strand, within CA265_RS15215 at 3828.199 kb on - strand, within CA265_RS15215 at 3828.212 kb on - strand, within CA265_RS15215 at 3828.212 kb on - strand, within CA265_RS15215 at 3828.251 kb on + strand, within CA265_RS15215 at 3828.252 kb on - strand, within CA265_RS15215 at 3828.252 kb on - strand, within CA265_RS15215 at 3828.330 kb on + strand, within CA265_RS15215 at 3828.337 kb on - strand, within CA265_RS15215 at 3828.409 kb on + strand, within CA265_RS15215 at 3828.410 kb on - strand, within CA265_RS15215 at 3828.410 kb on - strand, within CA265_RS15215 at 3828.440 kb on + strand, within CA265_RS15215 at 3828.441 kb on - strand, within CA265_RS15215 at 3828.441 kb on - strand at 3828.464 kb on - strand, within CA265_RS15215 at 3828.464 kb on - strand, within CA265_RS15215 at 3828.464 kb on - strand, within CA265_RS15215 at 3828.484 kb on + strand, within CA265_RS15215 at 3828.485 kb on - strand, within CA265_RS15215 at 3828.521 kb on + strand, within CA265_RS15215 at 3828.522 kb on - strand, within CA265_RS15215 at 3828.548 kb on - strand, within CA265_RS15215 at 3828.549 kb on + strand, within CA265_RS15215 at 3828.549 kb on + strand, within CA265_RS15215 at 3828.550 kb on - strand, within CA265_RS15215 at 3828.550 kb on - strand, within CA265_RS15215 at 3828.568 kb on + strand, within CA265_RS15215
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 3,826,092 - CA265_RS15205 0.28 -0.4 3,826,110 + CA265_RS15205 0.30 -1.1 3,826,118 + CA265_RS15205 0.31 -2.2 3,826,122 - CA265_RS15205 0.31 -1.1 3,826,193 - CA265_RS15205 0.37 -1.5 3,826,234 + CA265_RS15205 0.41 -0.9 3,826,271 - CA265_RS15205 0.44 -0.9 3,826,289 - CA265_RS15205 0.46 +0.0 3,826,308 + CA265_RS15205 0.47 -1.4 3,826,309 - CA265_RS15205 0.48 -0.2 3,826,309 - CA265_RS15205 0.48 -0.1 3,826,311 - CA265_RS15205 0.48 -1.3 3,826,426 + CA265_RS15205 0.58 -0.8 3,826,521 - CA265_RS15205 0.66 -0.0 3,826,591 + CA265_RS15205 0.72 -0.6 3,826,591 + CA265_RS15205 0.72 -0.9 3,826,592 - CA265_RS15205 0.72 -1.9 3,826,771 + CA265_RS15205 0.88 -0.8 3,826,772 - CA265_RS15205 0.88 -0.7 3,826,830 - -0.7 3,826,879 + -1.5 3,826,964 + -0.6 3,826,964 + +0.0 3,826,965 - -0.6 3,827,020 - -0.2 3,827,031 + CA265_RS15210 0.11 -0.5 3,827,032 - CA265_RS15210 0.11 +0.3 3,827,076 - CA265_RS15210 0.18 +0.2 3,827,082 - CA265_RS15210 0.18 -0.6 3,827,153 + CA265_RS15210 0.29 -0.6 3,827,223 + CA265_RS15210 0.39 -0.4 3,827,251 + CA265_RS15210 0.43 +0.6 3,827,292 + CA265_RS15210 0.49 +0.5 3,827,349 + CA265_RS15210 0.58 -1.0 3,827,508 - CA265_RS15210 0.81 -0.5 3,827,625 + +0.4 3,827,687 + -0.2 3,827,705 - +0.9 3,827,746 - -0.2 3,827,797 - +0.3 3,827,811 - +0.7 3,827,858 - -2.6 3,827,953 + CA265_RS15215 0.14 +0.4 3,827,984 + CA265_RS15215 0.16 +0.0 3,828,003 - CA265_RS15215 0.18 -0.0 3,828,041 + CA265_RS15215 0.21 -0.4 3,828,052 + CA265_RS15215 0.22 -0.9 3,828,053 - CA265_RS15215 0.22 +0.2 3,828,083 + CA265_RS15215 0.24 -0.0 3,828,198 + CA265_RS15215 0.33 -2.1 3,828,199 - CA265_RS15215 0.33 -0.8 3,828,212 - CA265_RS15215 0.34 -0.5 3,828,212 - CA265_RS15215 0.34 -0.5 3,828,251 + CA265_RS15215 0.37 -0.8 3,828,252 - CA265_RS15215 0.37 -0.2 3,828,252 - CA265_RS15215 0.37 -0.5 3,828,330 + CA265_RS15215 0.43 -1.7 3,828,337 - CA265_RS15215 0.44 -2.8 3,828,409 + CA265_RS15215 0.49 -0.4 3,828,410 - CA265_RS15215 0.49 -2.1 3,828,410 - CA265_RS15215 0.49 +0.3 3,828,440 + CA265_RS15215 0.51 -0.7 3,828,441 - CA265_RS15215 0.51 -0.0 3,828,441 - +1.4 3,828,464 - CA265_RS15215 0.53 -0.3 3,828,464 - CA265_RS15215 0.53 -0.7 3,828,464 - CA265_RS15215 0.53 +0.6 3,828,484 + CA265_RS15215 0.55 -0.4 3,828,485 - CA265_RS15215 0.55 -0.6 3,828,521 + CA265_RS15215 0.58 -1.7 3,828,522 - CA265_RS15215 0.58 -0.1 3,828,548 - CA265_RS15215 0.60 +0.1 3,828,549 + CA265_RS15215 0.60 +0.5 3,828,549 + CA265_RS15215 0.60 +1.4 3,828,550 - CA265_RS15215 0.60 +1.8 3,828,550 - CA265_RS15215 0.60 -0.8 3,828,568 + CA265_RS15215 0.61 -0.3
Or see this region's nucleotide sequence