Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09080 and CA265_RS09085 overlap by 4 nucleotides CA265_RS09085 and CA265_RS09090 are separated by 34 nucleotides CA265_RS09090 and CA265_RS09095 are separated by 288 nucleotides
CA265_RS09080: CA265_RS09080 - thioredoxin, at 2,262,962 to 2,263,258
_RS09080
CA265_RS09085: CA265_RS09085 - DNA repair nuclease, ligase-associated (TIGR04123) (from data) , at 2,263,255 to 2,263,893
_RS09085
CA265_RS09090: CA265_RS09090 - DNA ligase-associated DEXH box helicase, at 2,263,928 to 2,266,405
_RS09090
CA265_RS09095: CA265_RS09095 - ATP-dependent DNA ligase, at 2,266,694 to 2,268,283
_RS09095
Position (kb)
2263
2264
2265
2266
2267 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2262.958 kb on + strand at 2262.958 kb on + strand at 2262.958 kb on + strand at 2262.959 kb on - strand at 2262.982 kb on - strand at 2262.986 kb on - strand at 2263.081 kb on - strand, within CA265_RS09080 at 2263.144 kb on + strand, within CA265_RS09080 at 2263.145 kb on - strand, within CA265_RS09080 at 2263.159 kb on - strand, within CA265_RS09080 at 2263.293 kb on + strand at 2263.293 kb on + strand at 2263.323 kb on - strand, within CA265_RS09085 at 2263.337 kb on - strand, within CA265_RS09085 at 2263.376 kb on + strand, within CA265_RS09085 at 2263.423 kb on + strand, within CA265_RS09085 at 2263.455 kb on - strand, within CA265_RS09085 at 2263.585 kb on + strand, within CA265_RS09085 at 2263.586 kb on - strand, within CA265_RS09085 at 2263.651 kb on + strand, within CA265_RS09085 at 2263.651 kb on + strand, within CA265_RS09085 at 2263.652 kb on - strand, within CA265_RS09085 at 2263.669 kb on - strand, within CA265_RS09085 at 2263.701 kb on - strand, within CA265_RS09085 at 2263.795 kb on + strand at 2263.795 kb on + strand, within CA265_RS09085 at 2263.796 kb on - strand, within CA265_RS09085 at 2263.796 kb on - strand, within CA265_RS09085 at 2263.796 kb on - strand, within CA265_RS09085 at 2263.811 kb on - strand, within CA265_RS09085 at 2263.896 kb on - strand at 2263.896 kb on - strand at 2263.897 kb on + strand at 2263.906 kb on - strand at 2263.994 kb on - strand at 2264.009 kb on - strand at 2264.012 kb on + strand at 2264.012 kb on + strand at 2264.031 kb on - strand at 2264.060 kb on + strand at 2264.140 kb on - strand at 2264.167 kb on + strand at 2264.173 kb on - strand at 2264.278 kb on + strand, within CA265_RS09090 at 2264.327 kb on - strand, within CA265_RS09090 at 2264.365 kb on - strand, within CA265_RS09090 at 2264.435 kb on - strand, within CA265_RS09090 at 2264.436 kb on + strand, within CA265_RS09090 at 2264.546 kb on - strand, within CA265_RS09090 at 2264.546 kb on - strand, within CA265_RS09090 at 2264.634 kb on - strand, within CA265_RS09090 at 2264.783 kb on - strand, within CA265_RS09090 at 2264.859 kb on + strand, within CA265_RS09090 at 2264.880 kb on + strand, within CA265_RS09090 at 2264.921 kb on + strand, within CA265_RS09090 at 2264.921 kb on + strand, within CA265_RS09090 at 2265.029 kb on + strand, within CA265_RS09090 at 2265.030 kb on - strand, within CA265_RS09090 at 2265.035 kb on - strand, within CA265_RS09090 at 2265.053 kb on - strand, within CA265_RS09090 at 2265.094 kb on + strand, within CA265_RS09090 at 2265.106 kb on + strand, within CA265_RS09090 at 2265.133 kb on + strand, within CA265_RS09090 at 2265.163 kb on + strand, within CA265_RS09090 at 2265.164 kb on - strand, within CA265_RS09090 at 2265.199 kb on + strand, within CA265_RS09090 at 2265.199 kb on + strand, within CA265_RS09090 at 2265.371 kb on + strand, within CA265_RS09090 at 2265.404 kb on + strand, within CA265_RS09090 at 2265.416 kb on + strand, within CA265_RS09090 at 2265.433 kb on + strand, within CA265_RS09090 at 2265.442 kb on + strand, within CA265_RS09090 at 2265.443 kb on - strand, within CA265_RS09090 at 2265.478 kb on + strand, within CA265_RS09090 at 2265.478 kb on + strand, within CA265_RS09090 at 2265.509 kb on + strand, within CA265_RS09090 at 2265.509 kb on + strand, within CA265_RS09090 at 2265.509 kb on + strand, within CA265_RS09090 at 2265.637 kb on - strand, within CA265_RS09090 at 2265.641 kb on + strand, within CA265_RS09090 at 2265.641 kb on + strand, within CA265_RS09090 at 2265.683 kb on - strand, within CA265_RS09090 at 2265.888 kb on - strand, within CA265_RS09090 at 2265.934 kb on + strand, within CA265_RS09090 at 2265.934 kb on + strand, within CA265_RS09090 at 2265.934 kb on + strand, within CA265_RS09090 at 2265.935 kb on - strand, within CA265_RS09090 at 2265.935 kb on - strand, within CA265_RS09090 at 2265.954 kb on - strand, within CA265_RS09090 at 2266.003 kb on + strand, within CA265_RS09090 at 2266.106 kb on + strand, within CA265_RS09090 at 2266.195 kb on + strand at 2266.196 kb on - strand at 2266.218 kb on - strand at 2266.348 kb on + strand at 2266.413 kb on + strand at 2266.475 kb on + strand at 2266.521 kb on + strand at 2266.522 kb on - strand at 2266.712 kb on + strand at 2266.713 kb on - strand at 2266.738 kb on + strand at 2266.778 kb on - strand at 2266.778 kb on - strand at 2266.778 kb on - strand at 2266.807 kb on + strand at 2266.866 kb on - strand, within CA265_RS09095 at 2266.920 kb on - strand, within CA265_RS09095 at 2266.928 kb on - strand, within CA265_RS09095 at 2267.049 kb on + strand, within CA265_RS09095 at 2267.049 kb on + strand, within CA265_RS09095 at 2267.071 kb on + strand, within CA265_RS09095 at 2267.071 kb on + strand, within CA265_RS09095 at 2267.072 kb on - strand, within CA265_RS09095 at 2267.074 kb on - strand, within CA265_RS09095 at 2267.150 kb on - strand, within CA265_RS09095 at 2267.232 kb on - strand, within CA265_RS09095 at 2267.295 kb on - strand, within CA265_RS09095 at 2267.326 kb on - strand, within CA265_RS09095
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 2,262,958 + +0.2 2,262,958 + +0.6 2,262,958 + -0.2 2,262,959 - +0.7 2,262,982 - +0.9 2,262,986 - +0.1 2,263,081 - CA265_RS09080 0.40 +1.6 2,263,144 + CA265_RS09080 0.61 -0.4 2,263,145 - CA265_RS09080 0.62 -0.2 2,263,159 - CA265_RS09080 0.66 -1.2 2,263,293 + +0.2 2,263,293 + +0.5 2,263,323 - CA265_RS09085 0.11 -0.0 2,263,337 - CA265_RS09085 0.13 +0.1 2,263,376 + CA265_RS09085 0.19 +0.1 2,263,423 + CA265_RS09085 0.26 +0.1 2,263,455 - CA265_RS09085 0.31 -0.6 2,263,585 + CA265_RS09085 0.52 +0.1 2,263,586 - CA265_RS09085 0.52 -0.5 2,263,651 + CA265_RS09085 0.62 +0.5 2,263,651 + CA265_RS09085 0.62 +0.3 2,263,652 - CA265_RS09085 0.62 -0.0 2,263,669 - CA265_RS09085 0.65 +0.5 2,263,701 - CA265_RS09085 0.70 +0.7 2,263,795 + +1.6 2,263,795 + CA265_RS09085 0.85 -0.1 2,263,796 - CA265_RS09085 0.85 +0.1 2,263,796 - CA265_RS09085 0.85 +1.6 2,263,796 - CA265_RS09085 0.85 +0.4 2,263,811 - CA265_RS09085 0.87 +0.2 2,263,896 - -0.4 2,263,896 - +1.2 2,263,897 + -0.0 2,263,906 - +0.8 2,263,994 - +1.2 2,264,009 - -0.0 2,264,012 + -1.4 2,264,012 + +1.2 2,264,031 - -0.1 2,264,060 + -0.4 2,264,140 - +0.2 2,264,167 + +3.8 2,264,173 - -0.4 2,264,278 + CA265_RS09090 0.14 +0.1 2,264,327 - CA265_RS09090 0.16 -0.2 2,264,365 - CA265_RS09090 0.18 +0.5 2,264,435 - CA265_RS09090 0.20 +0.2 2,264,436 + CA265_RS09090 0.21 +0.2 2,264,546 - CA265_RS09090 0.25 -0.5 2,264,546 - CA265_RS09090 0.25 +0.5 2,264,634 - CA265_RS09090 0.28 +0.5 2,264,783 - CA265_RS09090 0.35 -1.3 2,264,859 + CA265_RS09090 0.38 +0.3 2,264,880 + CA265_RS09090 0.38 +0.2 2,264,921 + CA265_RS09090 0.40 -0.4 2,264,921 + CA265_RS09090 0.40 +0.1 2,265,029 + CA265_RS09090 0.44 +0.2 2,265,030 - CA265_RS09090 0.44 +0.4 2,265,035 - CA265_RS09090 0.45 -0.1 2,265,053 - CA265_RS09090 0.45 +0.3 2,265,094 + CA265_RS09090 0.47 +0.4 2,265,106 + CA265_RS09090 0.48 -0.1 2,265,133 + CA265_RS09090 0.49 +0.2 2,265,163 + CA265_RS09090 0.50 +0.4 2,265,164 - CA265_RS09090 0.50 +0.6 2,265,199 + CA265_RS09090 0.51 -0.7 2,265,199 + CA265_RS09090 0.51 +1.2 2,265,371 + CA265_RS09090 0.58 -0.6 2,265,404 + CA265_RS09090 0.60 +0.5 2,265,416 + CA265_RS09090 0.60 +0.1 2,265,433 + CA265_RS09090 0.61 -0.5 2,265,442 + CA265_RS09090 0.61 +0.6 2,265,443 - CA265_RS09090 0.61 -0.3 2,265,478 + CA265_RS09090 0.63 +0.1 2,265,478 + CA265_RS09090 0.63 +0.1 2,265,509 + CA265_RS09090 0.64 +1.2 2,265,509 + CA265_RS09090 0.64 +0.6 2,265,509 + CA265_RS09090 0.64 +0.7 2,265,637 - CA265_RS09090 0.69 -0.9 2,265,641 + CA265_RS09090 0.69 +0.2 2,265,641 + CA265_RS09090 0.69 +0.6 2,265,683 - CA265_RS09090 0.71 +0.6 2,265,888 - CA265_RS09090 0.79 +0.4 2,265,934 + CA265_RS09090 0.81 -1.0 2,265,934 + CA265_RS09090 0.81 +1.1 2,265,934 + CA265_RS09090 0.81 -0.9 2,265,935 - CA265_RS09090 0.81 -0.3 2,265,935 - CA265_RS09090 0.81 -0.8 2,265,954 - CA265_RS09090 0.82 -0.2 2,266,003 + CA265_RS09090 0.84 +0.4 2,266,106 + CA265_RS09090 0.88 +0.0 2,266,195 + -0.6 2,266,196 - -0.3 2,266,218 - -0.3 2,266,348 + +0.1 2,266,413 + +0.3 2,266,475 + +0.1 2,266,521 + +0.6 2,266,522 - +0.5 2,266,712 + -0.1 2,266,713 - -0.0 2,266,738 + -0.4 2,266,778 - -0.5 2,266,778 - +0.7 2,266,778 - -0.2 2,266,807 + -0.3 2,266,866 - CA265_RS09095 0.11 +0.2 2,266,920 - CA265_RS09095 0.14 +0.5 2,266,928 - CA265_RS09095 0.15 -0.7 2,267,049 + CA265_RS09095 0.22 +0.2 2,267,049 + CA265_RS09095 0.22 +0.0 2,267,071 + CA265_RS09095 0.24 -0.4 2,267,071 + CA265_RS09095 0.24 +0.1 2,267,072 - CA265_RS09095 0.24 -0.3 2,267,074 - CA265_RS09095 0.24 +1.0 2,267,150 - CA265_RS09095 0.29 +0.4 2,267,232 - CA265_RS09095 0.34 +0.3 2,267,295 - CA265_RS09095 0.38 -0.2 2,267,326 - CA265_RS09095 0.40 +1.2
Or see this region's nucleotide sequence