Strain Fitness in Pedobacter sp. GW460-11-11-14-LB5 around CA265_RS02150

Experiment: R2A with Spectinomycin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCA265_RS02145 and CA265_RS02150 are separated by 757 nucleotidesCA265_RS02150 and CA265_RS02155 are separated by 86 nucleotidesCA265_RS02155 and CA265_RS02160 are separated by 22 nucleotides CA265_RS02145: CA265_RS02145 - 1,4-alpha-glucan branching enzyme, at 541,505 to 543,469 _RS02145 CA265_RS02150: CA265_RS02150 - hypothetical protein, at 544,227 to 546,386 _RS02150 CA265_RS02155: CA265_RS02155 - hypothetical protein, at 546,473 to 547,144 _RS02155 CA265_RS02160: CA265_RS02160 - hypothetical protein, at 547,167 to 548,108 _RS02160 Position (kb) 544 545 546 547Strain fitness (log2 ratio) -2 -1 0 1 2 3at 543.235 kb on + strand, within CA265_RS02145at 543.337 kb on + strandat 543.342 kb on + strandat 543.343 kb on - strandat 543.343 kb on - strandat 543.377 kb on - strandat 543.377 kb on - strandat 543.377 kb on - strandat 543.378 kb on + strandat 543.378 kb on + strandat 543.379 kb on - strandat 543.379 kb on - strandat 543.379 kb on - strandat 543.399 kb on - strandat 543.400 kb on + strandat 543.401 kb on - strandat 543.421 kb on - strandat 543.485 kb on - strandat 543.486 kb on + strandat 543.486 kb on + strandat 543.487 kb on - strandat 543.511 kb on + strandat 543.593 kb on - strandat 543.645 kb on + strandat 543.660 kb on + strandat 543.748 kb on + strandat 543.757 kb on + strandat 543.772 kb on + strandat 543.789 kb on + strandat 543.800 kb on + strandat 543.820 kb on + strandat 543.951 kb on - strandat 543.959 kb on + strandat 543.959 kb on + strandat 543.963 kb on + strandat 543.964 kb on - strandat 544.132 kb on - strandat 544.132 kb on - strandat 544.236 kb on + strandat 544.236 kb on + strandat 544.293 kb on + strandat 544.294 kb on - strandat 544.306 kb on + strandat 544.374 kb on - strandat 544.483 kb on + strand, within CA265_RS02150at 544.484 kb on - strand, within CA265_RS02150at 544.493 kb on + strand, within CA265_RS02150at 544.532 kb on - strand, within CA265_RS02150at 544.568 kb on + strandat 544.568 kb on + strand, within CA265_RS02150at 544.655 kb on + strand, within CA265_RS02150at 544.656 kb on - strand, within CA265_RS02150at 544.744 kb on + strand, within CA265_RS02150at 544.769 kb on + strand, within CA265_RS02150at 544.778 kb on - strand, within CA265_RS02150at 544.801 kb on - strand, within CA265_RS02150at 544.814 kb on - strand, within CA265_RS02150at 544.852 kb on + strand, within CA265_RS02150at 544.852 kb on + strand, within CA265_RS02150at 544.905 kb on - strand, within CA265_RS02150at 544.905 kb on - strand, within CA265_RS02150at 544.905 kb on - strand, within CA265_RS02150at 544.951 kb on - strand, within CA265_RS02150at 544.962 kb on + strand, within CA265_RS02150at 544.993 kb on - strand, within CA265_RS02150at 544.997 kb on - strand, within CA265_RS02150at 544.998 kb on - strand, within CA265_RS02150at 545.039 kb on - strand, within CA265_RS02150at 545.104 kb on + strand, within CA265_RS02150at 545.104 kb on + strand, within CA265_RS02150at 545.105 kb on - strand, within CA265_RS02150at 545.105 kb on - strand, within CA265_RS02150at 545.156 kb on + strand, within CA265_RS02150at 545.199 kb on + strand, within CA265_RS02150at 545.230 kb on - strand, within CA265_RS02150at 545.230 kb on - strand, within CA265_RS02150at 545.247 kb on - strand, within CA265_RS02150at 545.340 kb on - strand, within CA265_RS02150at 545.415 kb on + strand, within CA265_RS02150at 545.415 kb on + strand, within CA265_RS02150at 545.425 kb on - strand, within CA265_RS02150at 545.449 kb on + strand, within CA265_RS02150at 545.450 kb on - strand, within CA265_RS02150at 545.469 kb on + strand, within CA265_RS02150at 545.472 kb on - strand, within CA265_RS02150at 545.484 kb on - strand, within CA265_RS02150at 545.503 kb on - strand, within CA265_RS02150at 545.526 kb on - strand, within CA265_RS02150at 545.532 kb on + strand, within CA265_RS02150at 545.633 kb on + strand, within CA265_RS02150at 545.634 kb on - strand, within CA265_RS02150at 545.720 kb on + strand, within CA265_RS02150at 545.727 kb on + strand, within CA265_RS02150at 545.728 kb on - strand, within CA265_RS02150at 545.728 kb on - strand, within CA265_RS02150at 545.729 kb on + strand, within CA265_RS02150at 545.730 kb on - strand, within CA265_RS02150at 545.730 kb on - strand, within CA265_RS02150at 545.730 kb on - strand, within CA265_RS02150at 545.795 kb on + strand, within CA265_RS02150at 545.796 kb on - strand, within CA265_RS02150at 545.882 kb on + strand, within CA265_RS02150at 545.959 kb on + strand, within CA265_RS02150at 545.967 kb on - strand, within CA265_RS02150at 545.978 kb on + strand, within CA265_RS02150at 546.013 kb on - strand, within CA265_RS02150at 546.032 kb on + strand, within CA265_RS02150at 546.059 kb on + strand, within CA265_RS02150at 546.102 kb on - strand, within CA265_RS02150at 546.125 kb on + strand, within CA265_RS02150at 546.126 kb on - strand, within CA265_RS02150at 546.126 kb on - strand, within CA265_RS02150at 546.129 kb on - strand, within CA265_RS02150at 546.149 kb on + strand, within CA265_RS02150at 546.243 kb on - strandat 546.279 kb on + strandat 546.280 kb on - strandat 546.373 kb on + strandat 546.378 kb on - strandat 546.378 kb on - strandat 546.385 kb on - strandat 546.418 kb on + strandat 546.453 kb on + strandat 546.465 kb on + strandat 546.465 kb on + strandat 546.482 kb on - strandat 546.517 kb on - strandat 546.519 kb on - strandat 546.567 kb on - strand, within CA265_RS02155at 546.586 kb on + strand, within CA265_RS02155at 546.631 kb on - strand, within CA265_RS02155at 546.632 kb on + strand, within CA265_RS02155at 546.633 kb on - strand, within CA265_RS02155at 546.633 kb on - strand, within CA265_RS02155at 546.692 kb on + strand, within CA265_RS02155at 546.693 kb on - strand, within CA265_RS02155at 546.748 kb on + strand, within CA265_RS02155at 546.749 kb on - strand, within CA265_RS02155at 546.779 kb on - strand, within CA265_RS02155at 546.779 kb on - strand, within CA265_RS02155at 546.781 kb on + strand, within CA265_RS02155at 546.842 kb on - strand, within CA265_RS02155at 546.870 kb on + strand, within CA265_RS02155at 546.893 kb on + strand, within CA265_RS02155at 546.893 kb on + strand, within CA265_RS02155at 546.894 kb on - strand, within CA265_RS02155at 546.912 kb on + strand, within CA265_RS02155at 546.913 kb on - strand, within CA265_RS02155at 546.928 kb on - strand, within CA265_RS02155at 547.007 kb on + strand, within CA265_RS02155at 547.102 kb on + strandat 547.117 kb on + strandat 547.126 kb on + strandat 547.126 kb on + strandat 547.127 kb on - strandat 547.142 kb on + strandat 547.149 kb on + strandat 547.193 kb on - strandat 547.256 kb on + strandat 547.347 kb on - strand, within CA265_RS02160at 547.372 kb on - strand, within CA265_RS02160

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml
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543,235 + CA265_RS02145 0.88 -1.0
543,337 + -1.8
543,342 + -0.4
543,343 - -1.1
543,343 - -0.9
543,377 - -1.2
543,377 - -0.5
543,377 - -0.7
543,378 + +0.6
543,378 + -1.6
543,379 - -1.0
543,379 - -2.4
543,379 - -1.2
543,399 - -1.2
543,400 + -1.1
543,401 - -0.4
543,421 - -2.3
543,485 - +0.3
543,486 + -1.2
543,486 + -1.3
543,487 - -0.3
543,511 + -0.7
543,593 - -1.2
543,645 + +0.4
543,660 + +0.3
543,748 + +0.1
543,757 + +0.2
543,772 + -0.2
543,789 + +0.4
543,800 + +0.4
543,820 + +0.4
543,951 - -0.0
543,959 + -0.2
543,959 + +0.7
543,963 + -0.1
543,964 - -1.0
544,132 - -0.1
544,132 - +0.0
544,236 + +0.1
544,236 + -0.5
544,293 + -0.9
544,294 - -1.2
544,306 + +0.5
544,374 - -0.2
544,483 + CA265_RS02150 0.12 +0.4
544,484 - CA265_RS02150 0.12 -0.9
544,493 + CA265_RS02150 0.12 +0.4
544,532 - CA265_RS02150 0.14 +2.9
544,568 + +2.4
544,568 + CA265_RS02150 0.16 +0.2
544,655 + CA265_RS02150 0.20 -0.0
544,656 - CA265_RS02150 0.20 -0.6
544,744 + CA265_RS02150 0.24 +0.3
544,769 + CA265_RS02150 0.25 -0.2
544,778 - CA265_RS02150 0.26 +0.7
544,801 - CA265_RS02150 0.27 -1.3
544,814 - CA265_RS02150 0.27 +0.7
544,852 + CA265_RS02150 0.29 +0.3
544,852 + CA265_RS02150 0.29 +0.6
544,905 - CA265_RS02150 0.31 +0.5
544,905 - CA265_RS02150 0.31 -0.5
544,905 - CA265_RS02150 0.31 +1.1
544,951 - CA265_RS02150 0.34 -1.1
544,962 + CA265_RS02150 0.34 +0.5
544,993 - CA265_RS02150 0.35 +0.2
544,997 - CA265_RS02150 0.36 +0.8
544,998 - CA265_RS02150 0.36 -0.2
545,039 - CA265_RS02150 0.38 +0.0
545,104 + CA265_RS02150 0.41 -0.1
545,104 + CA265_RS02150 0.41 +1.0
545,105 - CA265_RS02150 0.41 +0.4
545,105 - CA265_RS02150 0.41 +0.4
545,156 + CA265_RS02150 0.43 -0.3
545,199 + CA265_RS02150 0.45 -1.0
545,230 - CA265_RS02150 0.46 +0.5
545,230 - CA265_RS02150 0.46 -0.3
545,247 - CA265_RS02150 0.47 -1.8
545,340 - CA265_RS02150 0.52 +0.3
545,415 + CA265_RS02150 0.55 -0.6
545,415 + CA265_RS02150 0.55 +0.3
545,425 - CA265_RS02150 0.55 +0.3
545,449 + CA265_RS02150 0.57 +0.4
545,450 - CA265_RS02150 0.57 +0.2
545,469 + CA265_RS02150 0.57 +0.7
545,472 - CA265_RS02150 0.58 -0.2
545,484 - CA265_RS02150 0.58 +0.6
545,503 - CA265_RS02150 0.59 +2.4
545,526 - CA265_RS02150 0.60 +0.5
545,532 + CA265_RS02150 0.60 +1.1
545,633 + CA265_RS02150 0.65 +0.8
545,634 - CA265_RS02150 0.65 -0.2
545,720 + CA265_RS02150 0.69 -0.0
545,727 + CA265_RS02150 0.69 +1.1
545,728 - CA265_RS02150 0.69 -0.4
545,728 - CA265_RS02150 0.69 -0.3
545,729 + CA265_RS02150 0.70 -0.5
545,730 - CA265_RS02150 0.70 -2.0
545,730 - CA265_RS02150 0.70 -0.6
545,730 - CA265_RS02150 0.70 +0.4
545,795 + CA265_RS02150 0.73 +0.8
545,796 - CA265_RS02150 0.73 -1.1
545,882 + CA265_RS02150 0.77 +0.4
545,959 + CA265_RS02150 0.80 +0.5
545,967 - CA265_RS02150 0.81 -0.5
545,978 + CA265_RS02150 0.81 -0.6
546,013 - CA265_RS02150 0.83 +0.2
546,032 + CA265_RS02150 0.84 +0.2
546,059 + CA265_RS02150 0.85 -0.8
546,102 - CA265_RS02150 0.87 -0.1
546,125 + CA265_RS02150 0.88 -0.3
546,126 - CA265_RS02150 0.88 -0.1
546,126 - CA265_RS02150 0.88 -0.1
546,129 - CA265_RS02150 0.88 -0.2
546,149 + CA265_RS02150 0.89 +1.5
546,243 - +0.2
546,279 + -0.6
546,280 - +0.1
546,373 + +0.0
546,378 - -1.2
546,378 - +0.3
546,385 - -0.4
546,418 + +0.4
546,453 + +1.1
546,465 + -1.0
546,465 + -0.4
546,482 - -0.1
546,517 - -0.3
546,519 - -0.4
546,567 - CA265_RS02155 0.14 -0.3
546,586 + CA265_RS02155 0.17 +0.4
546,631 - CA265_RS02155 0.24 -0.9
546,632 + CA265_RS02155 0.24 -0.4
546,633 - CA265_RS02155 0.24 +1.4
546,633 - CA265_RS02155 0.24 -0.5
546,692 + CA265_RS02155 0.33 -0.1
546,693 - CA265_RS02155 0.33 -0.8
546,748 + CA265_RS02155 0.41 +0.4
546,749 - CA265_RS02155 0.41 -0.6
546,779 - CA265_RS02155 0.46 -0.5
546,779 - CA265_RS02155 0.46 -0.1
546,781 + CA265_RS02155 0.46 +0.0
546,842 - CA265_RS02155 0.55 +0.4
546,870 + CA265_RS02155 0.59 -0.5
546,893 + CA265_RS02155 0.62 +1.0
546,893 + CA265_RS02155 0.62 -0.9
546,894 - CA265_RS02155 0.63 -0.1
546,912 + CA265_RS02155 0.65 -0.6
546,913 - CA265_RS02155 0.65 -0.8
546,928 - CA265_RS02155 0.68 -1.0
547,007 + CA265_RS02155 0.79 +0.7
547,102 + -0.8
547,117 + -0.7
547,126 + -0.5
547,126 + -0.5
547,127 - -0.6
547,142 + -0.0
547,149 + -0.2
547,193 - +0.8
547,256 + -0.5
547,347 - CA265_RS02160 0.19 -0.2
547,372 - CA265_RS02160 0.22 +0.1

Or see this region's nucleotide sequence