Experiment: CDM1 without Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17360 and BBR_RS17365 are separated by 5 nucleotides BBR_RS17365 and BBR_RS17370 overlap by 1 nucleotides BBR_RS17370 and BBR_RS17375 are separated by 18 nucleotides BBR_RS17375 and BBR_RS17380 are separated by 52 nucleotides
BBR_RS17360: BBR_RS17360 - amino acid ABC transporter permease, at 1,673,346 to 1,674,446
_RS17360
BBR_RS17365: BBR_RS17365 - ABC transporter permease, at 1,674,452 to 1,675,129
_RS17365
BBR_RS17370: BBR_RS17370 - ABC transporter substrate-binding protein, at 1,675,129 to 1,675,968
_RS17370
BBR_RS17375: BBR_RS17375 - amino acid ABC transporter ATP-binding protein, at 1,675,987 to 1,676,829
_RS17375
BBR_RS17380: BBR_RS17380 - hypothetical protein, at 1,676,882 to 1,677,433
_RS17380
Position (kb)
1675
1676 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1674.141 kb on - strand, within BBR_RS17360 at 1674.145 kb on + strand, within BBR_RS17360 at 1674.145 kb on + strand, within BBR_RS17360 at 1674.169 kb on - strand, within BBR_RS17360 at 1674.181 kb on + strand, within BBR_RS17360 at 1674.189 kb on - strand, within BBR_RS17360 at 1674.214 kb on - strand, within BBR_RS17360 at 1674.242 kb on - strand, within BBR_RS17360 at 1674.242 kb on - strand, within BBR_RS17360 at 1674.255 kb on + strand, within BBR_RS17360 at 1674.265 kb on - strand, within BBR_RS17360 at 1674.270 kb on + strand, within BBR_RS17360 at 1674.278 kb on + strand, within BBR_RS17360 at 1674.313 kb on - strand, within BBR_RS17360 at 1674.344 kb on - strand at 1674.344 kb on - strand at 1674.360 kb on - strand at 1674.380 kb on + strand at 1674.396 kb on + strand at 1674.396 kb on + strand at 1674.420 kb on - strand at 1674.455 kb on + strand at 1674.455 kb on + strand at 1674.465 kb on - strand at 1674.465 kb on - strand at 1674.509 kb on + strand at 1674.553 kb on - strand, within BBR_RS17365 at 1674.633 kb on + strand, within BBR_RS17365 at 1674.668 kb on - strand, within BBR_RS17365 at 1674.738 kb on - strand, within BBR_RS17365 at 1674.753 kb on + strand, within BBR_RS17365 at 1674.753 kb on + strand, within BBR_RS17365 at 1674.761 kb on - strand, within BBR_RS17365 at 1674.816 kb on + strand, within BBR_RS17365 at 1674.816 kb on + strand, within BBR_RS17365 at 1674.828 kb on - strand, within BBR_RS17365 at 1674.945 kb on + strand, within BBR_RS17365 at 1674.963 kb on - strand, within BBR_RS17365 at 1674.973 kb on - strand, within BBR_RS17365 at 1674.986 kb on - strand, within BBR_RS17365 at 1675.018 kb on + strand, within BBR_RS17365 at 1675.035 kb on + strand, within BBR_RS17365 at 1675.040 kb on + strand, within BBR_RS17365 at 1675.050 kb on + strand, within BBR_RS17365 at 1675.063 kb on + strand at 1675.063 kb on + strand at 1675.073 kb on + strand at 1675.090 kb on - strand at 1675.100 kb on - strand at 1675.109 kb on - strand at 1675.127 kb on + strand at 1675.146 kb on + strand at 1675.146 kb on + strand at 1675.169 kb on - strand at 1675.173 kb on + strand at 1675.178 kb on + strand at 1675.181 kb on - strand at 1675.186 kb on - strand at 1675.201 kb on - strand at 1675.214 kb on + strand, within BBR_RS17370 at 1675.229 kb on - strand, within BBR_RS17370 at 1675.229 kb on - strand, within BBR_RS17370 at 1675.243 kb on + strand, within BBR_RS17370 at 1675.243 kb on + strand, within BBR_RS17370 at 1675.278 kb on - strand, within BBR_RS17370 at 1675.280 kb on + strand, within BBR_RS17370 at 1675.315 kb on - strand, within BBR_RS17370 at 1675.337 kb on + strand, within BBR_RS17370 at 1675.345 kb on - strand, within BBR_RS17370 at 1675.345 kb on - strand, within BBR_RS17370 at 1675.345 kb on - strand, within BBR_RS17370 at 1675.345 kb on - strand, within BBR_RS17370 at 1675.346 kb on - strand, within BBR_RS17370 at 1675.363 kb on + strand, within BBR_RS17370 at 1675.371 kb on - strand, within BBR_RS17370 at 1675.377 kb on + strand, within BBR_RS17370 at 1675.381 kb on - strand, within BBR_RS17370 at 1675.408 kb on - strand, within BBR_RS17370 at 1675.429 kb on + strand, within BBR_RS17370 at 1675.553 kb on - strand, within BBR_RS17370 at 1675.583 kb on + strand, within BBR_RS17370 at 1675.614 kb on - strand, within BBR_RS17370 at 1675.614 kb on - strand, within BBR_RS17370 at 1675.692 kb on - strand, within BBR_RS17370 at 1675.692 kb on - strand, within BBR_RS17370 at 1675.787 kb on - strand, within BBR_RS17370 at 1675.831 kb on - strand, within BBR_RS17370 at 1675.895 kb on + strand at 1675.895 kb on + strand at 1675.898 kb on + strand at 1675.898 kb on + strand at 1675.905 kb on + strand at 1675.906 kb on - strand at 1675.931 kb on + strand at 1675.952 kb on - strand at 1675.988 kb on + strand at 1676.017 kb on - strand at 1676.057 kb on + strand at 1676.065 kb on - strand at 1676.119 kb on - strand, within BBR_RS17375 at 1676.142 kb on + strand, within BBR_RS17375 at 1676.225 kb on - strand, within BBR_RS17375 at 1676.240 kb on + strand, within BBR_RS17375 at 1676.246 kb on + strand, within BBR_RS17375 at 1676.246 kb on + strand, within BBR_RS17375 at 1676.248 kb on - strand, within BBR_RS17375 at 1676.248 kb on - strand, within BBR_RS17375 at 1676.272 kb on + strand, within BBR_RS17375 at 1676.386 kb on - strand, within BBR_RS17375 at 1676.386 kb on - strand, within BBR_RS17375 at 1676.472 kb on - strand, within BBR_RS17375 at 1676.489 kb on - strand, within BBR_RS17375 at 1676.503 kb on + strand, within BBR_RS17375 at 1676.503 kb on + strand, within BBR_RS17375 at 1676.511 kb on - strand, within BBR_RS17375 at 1676.515 kb on + strand, within BBR_RS17375 at 1676.525 kb on + strand, within BBR_RS17375 at 1676.615 kb on + strand, within BBR_RS17375 at 1676.662 kb on + strand, within BBR_RS17375 at 1676.704 kb on - strand, within BBR_RS17375 at 1676.722 kb on + strand, within BBR_RS17375 at 1676.760 kb on - strand at 1676.768 kb on + strand at 1676.853 kb on - strand at 1676.853 kb on - strand at 1676.867 kb on + strand at 1676.892 kb on + strand at 1676.938 kb on + strand, within BBR_RS17380
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Serine remove 1,674,141 - BBR_RS17360 0.72 -2.4 1,674,145 + BBR_RS17360 0.73 -3.7 1,674,145 + BBR_RS17360 0.73 -3.9 1,674,169 - BBR_RS17360 0.75 -2.1 1,674,181 + BBR_RS17360 0.76 -2.5 1,674,189 - BBR_RS17360 0.77 -0.9 1,674,214 - BBR_RS17360 0.79 -5.0 1,674,242 - BBR_RS17360 0.81 -5.3 1,674,242 - BBR_RS17360 0.81 -4.7 1,674,255 + BBR_RS17360 0.83 -3.6 1,674,265 - BBR_RS17360 0.83 -3.2 1,674,270 + BBR_RS17360 0.84 -1.4 1,674,278 + BBR_RS17360 0.85 -4.4 1,674,313 - BBR_RS17360 0.88 -2.5 1,674,344 - -1.8 1,674,344 - -1.5 1,674,360 - -2.7 1,674,380 + -2.7 1,674,396 + -2.2 1,674,396 + -1.8 1,674,420 - -4.0 1,674,455 + -2.5 1,674,455 + -2.8 1,674,465 - -3.4 1,674,465 - -3.0 1,674,509 + -1.0 1,674,553 - BBR_RS17365 0.15 -3.6 1,674,633 + BBR_RS17365 0.27 -3.2 1,674,668 - BBR_RS17365 0.32 -1.8 1,674,738 - BBR_RS17365 0.42 -2.8 1,674,753 + BBR_RS17365 0.44 -3.1 1,674,753 + BBR_RS17365 0.44 -3.1 1,674,761 - BBR_RS17365 0.46 -1.7 1,674,816 + BBR_RS17365 0.54 -2.6 1,674,816 + BBR_RS17365 0.54 -3.2 1,674,828 - BBR_RS17365 0.55 -3.2 1,674,945 + BBR_RS17365 0.73 -1.3 1,674,963 - BBR_RS17365 0.75 -2.5 1,674,973 - BBR_RS17365 0.77 -3.2 1,674,986 - BBR_RS17365 0.79 -3.2 1,675,018 + BBR_RS17365 0.83 -1.9 1,675,035 + BBR_RS17365 0.86 -1.5 1,675,040 + BBR_RS17365 0.87 -1.7 1,675,050 + BBR_RS17365 0.88 -2.2 1,675,063 + -2.6 1,675,063 + -3.5 1,675,073 + -2.7 1,675,090 - -2.0 1,675,100 - -3.4 1,675,109 - -3.9 1,675,127 + +0.2 1,675,146 + -1.8 1,675,146 + -2.8 1,675,169 - -0.9 1,675,173 + -1.6 1,675,178 + -1.4 1,675,181 - +0.2 1,675,186 - -1.7 1,675,201 - -0.5 1,675,214 + BBR_RS17370 0.10 -1.9 1,675,229 - BBR_RS17370 0.12 -3.9 1,675,229 - BBR_RS17370 0.12 -1.5 1,675,243 + BBR_RS17370 0.14 -2.4 1,675,243 + BBR_RS17370 0.14 -3.3 1,675,278 - BBR_RS17370 0.18 -3.2 1,675,280 + BBR_RS17370 0.18 -3.6 1,675,315 - BBR_RS17370 0.22 -1.9 1,675,337 + BBR_RS17370 0.25 -3.4 1,675,345 - BBR_RS17370 0.26 -2.3 1,675,345 - BBR_RS17370 0.26 -4.1 1,675,345 - BBR_RS17370 0.26 -3.4 1,675,345 - BBR_RS17370 0.26 -3.4 1,675,346 - BBR_RS17370 0.26 -1.4 1,675,363 + BBR_RS17370 0.28 -2.5 1,675,371 - BBR_RS17370 0.29 -4.1 1,675,377 + BBR_RS17370 0.30 -3.7 1,675,381 - BBR_RS17370 0.30 -2.5 1,675,408 - BBR_RS17370 0.33 -2.6 1,675,429 + BBR_RS17370 0.36 -2.7 1,675,553 - BBR_RS17370 0.50 -3.1 1,675,583 + BBR_RS17370 0.54 -1.5 1,675,614 - BBR_RS17370 0.58 -2.8 1,675,614 - BBR_RS17370 0.58 -1.9 1,675,692 - BBR_RS17370 0.67 -2.6 1,675,692 - BBR_RS17370 0.67 -3.7 1,675,787 - BBR_RS17370 0.78 -3.1 1,675,831 - BBR_RS17370 0.84 -2.2 1,675,895 + -3.2 1,675,895 + -3.6 1,675,898 + -3.2 1,675,898 + -3.8 1,675,905 + -3.5 1,675,906 - -2.4 1,675,931 + -0.6 1,675,952 - -0.9 1,675,988 + -4.0 1,676,017 - -2.4 1,676,057 + -2.4 1,676,065 - -3.1 1,676,119 - BBR_RS17375 0.16 -2.5 1,676,142 + BBR_RS17375 0.18 -1.8 1,676,225 - BBR_RS17375 0.28 -1.9 1,676,240 + BBR_RS17375 0.30 -3.1 1,676,246 + BBR_RS17375 0.31 -1.7 1,676,246 + BBR_RS17375 0.31 -2.4 1,676,248 - BBR_RS17375 0.31 -4.2 1,676,248 - BBR_RS17375 0.31 -4.0 1,676,272 + BBR_RS17375 0.34 -2.3 1,676,386 - BBR_RS17375 0.47 -3.0 1,676,386 - BBR_RS17375 0.47 -4.2 1,676,472 - BBR_RS17375 0.58 -2.3 1,676,489 - BBR_RS17375 0.60 -4.1 1,676,503 + BBR_RS17375 0.61 -0.5 1,676,503 + BBR_RS17375 0.61 -0.6 1,676,511 - BBR_RS17375 0.62 -4.5 1,676,515 + BBR_RS17375 0.63 -0.5 1,676,525 + BBR_RS17375 0.64 -2.9 1,676,615 + BBR_RS17375 0.74 -3.5 1,676,662 + BBR_RS17375 0.80 -2.8 1,676,704 - BBR_RS17375 0.85 -3.0 1,676,722 + BBR_RS17375 0.87 -4.2 1,676,760 - -1.4 1,676,768 + -3.6 1,676,853 - -3.0 1,676,853 - -0.1 1,676,867 + +1.2 1,676,892 + +0.5 1,676,938 + BBR_RS17380 0.10 -0.3
Or see this region's nucleotide sequence