Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2763 and Shewana3_2764 are separated by 58 nucleotides Shewana3_2764 and fabG are separated by 207 nucleotides fabG and Shewana3_2766 are separated by 19 nucleotides
Shewana3_2763: Shewana3_2763 - peptidase S9 prolyl oligopeptidase (RefSeq), at 3,301,029 to 3,303,062
_2763
Shewana3_2764: Shewana3_2764 - hypothetical protein (RefSeq), at 3,303,121 to 3,303,363
_2764
Shewana3_2765: fabG - 3-ketoacyl-(acyl-carrier-protein) reductase (RefSeq), at 3,303,571 to 3,304,329
fabG
Shewana3_2766: Shewana3_2766 - 3-hydroxyisobutyrate dehydrogenase (RefSeq), at 3,304,349 to 3,305,251
_2766
Position (kb)
3303
3304 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 3302.195 kb on + strand, within Shewana3_2763 at 3302.410 kb on - strand, within Shewana3_2763 at 3302.481 kb on + strand, within Shewana3_2763 at 3302.546 kb on + strand, within Shewana3_2763 at 3302.815 kb on + strand, within Shewana3_2763 at 3302.833 kb on - strand, within Shewana3_2763 at 3303.344 kb on + strand at 3303.392 kb on - strand at 3303.642 kb on + strand at 3303.750 kb on + strand, within fabG at 3303.758 kb on - strand, within fabG
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 3,302,195 + Shewana3_2763 0.57 +0.6 3,302,410 - Shewana3_2763 0.68 +0.7 3,302,481 + Shewana3_2763 0.71 +0.5 3,302,546 + Shewana3_2763 0.75 +0.3 3,302,815 + Shewana3_2763 0.88 +0.4 3,302,833 - Shewana3_2763 0.89 -0.8 3,303,344 + +0.9 3,303,392 - +0.3 3,303,642 + +5.0 3,303,750 + fabG Shewana3_2765 0.24 +3.3 3,303,758 - fabG Shewana3_2765 0.25 +4.4
Or see this region's nucleotide sequence