Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysS and Shewana3_2665 are separated by 134 nucleotides Shewana3_2665 and Shewana3_2666 are separated by 9 nucleotides Shewana3_2666 and Shewana3_2667 are separated by 108 nucleotides
Shewana3_2664: cysS - cysteinyl-tRNA synthetase (RefSeq), at 3,180,798 to 3,182,183
cysS
Shewana3_2665: Shewana3_2665 - peptidyl-prolyl cis-trans isomerase, cyclophilin type (RefSeq), at 3,182,318 to 3,182,809
_2665
Shewana3_2666: Shewana3_2666 - UDP-2,3-diacylglucosamine hydrolase (RefSeq), at 3,182,819 to 3,183,538
_2666
Shewana3_2667: Shewana3_2667 - tRNA hydroxylase (RefSeq), at 3,183,647 to 3,184,417
_2667
Position (kb)
3182
3183
3184 Strain fitness (log2 ratio)
-2
-1
0
1 at 3183.752 kb on - strand, within Shewana3_2667 at 3183.889 kb on + strand, within Shewana3_2667 at 3184.075 kb on - strand, within Shewana3_2667 at 3184.114 kb on + strand, within Shewana3_2667 at 3184.225 kb on - strand, within Shewana3_2667 at 3184.238 kb on + strand, within Shewana3_2667 at 3184.475 kb on + strand at 3184.475 kb on + strand at 3184.482 kb on + strand at 3184.482 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 3,183,752 - Shewana3_2667 0.14 -1.8 3,183,889 + Shewana3_2667 0.31 -2.4 3,184,075 - Shewana3_2667 0.56 -0.3 3,184,114 + Shewana3_2667 0.61 +0.2 3,184,225 - Shewana3_2667 0.75 -0.5 3,184,238 + Shewana3_2667 0.77 +0.9 3,184,475 + -0.4 3,184,475 + -0.7 3,184,482 + -1.2 3,184,482 + -0.2
Or see this region's nucleotide sequence