Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0424 and pdhR are separated by 325 nucleotides pdhR and aceE are separated by 80 nucleotides
Shewana3_0424: Shewana3_0424 - regulatory protein AmpE (RefSeq), at 483,246 to 484,097
_0424
Shewana3_0425: pdhR - transcriptional regulator PdhR (RefSeq), at 484,423 to 485,175
pdhR
Shewana3_0426: aceE - pyruvate dehydrogenase subunit E1 (RefSeq), at 485,256 to 487,922
aceE
Position (kb) 484
485
486 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 483.732 kb on + strand, within Shewana3_0424 at 483.732 kb on + strand, within Shewana3_0424 at 483.737 kb on + strand, within Shewana3_0424 at 483.740 kb on - strand, within Shewana3_0424 at 483.740 kb on - strand, within Shewana3_0424 at 483.740 kb on - strand, within Shewana3_0424 at 483.745 kb on - strand, within Shewana3_0424 at 483.745 kb on - strand, within Shewana3_0424 at 483.745 kb on - strand, within Shewana3_0424 at 483.860 kb on + strand, within Shewana3_0424 at 483.872 kb on + strand, within Shewana3_0424 at 483.876 kb on + strand, within Shewana3_0424 at 483.892 kb on - strand, within Shewana3_0424 at 483.960 kb on + strand, within Shewana3_0424 at 484.039 kb on + strand at 484.039 kb on + strand at 484.039 kb on + strand at 484.047 kb on - strand at 484.165 kb on + strand at 484.233 kb on + strand at 484.513 kb on + strand, within pdhR at 484.918 kb on + strand, within pdhR at 485.241 kb on + strand at 485.271 kb on + strand at 485.278 kb on + strand at 485.314 kb on - strand at 485.325 kb on + strand at 485.408 kb on + strand at 485.416 kb on - strand at 485.800 kb on - strand, within aceE at 485.882 kb on + strand, within aceE at 485.989 kb on - strand, within aceE at 486.036 kb on - strand, within aceE at 486.068 kb on + strand, within aceE at 486.068 kb on + strand, within aceE
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 483,732 + Shewana3_0424 0.57 -0.8 483,732 + Shewana3_0424 0.57 +0.9 483,737 + Shewana3_0424 0.58 -0.1 483,740 - Shewana3_0424 0.58 +0.7 483,740 - Shewana3_0424 0.58 +0.3 483,740 - Shewana3_0424 0.58 -0.9 483,745 - Shewana3_0424 0.59 -0.8 483,745 - Shewana3_0424 0.59 +1.0 483,745 - Shewana3_0424 0.59 +0.2 483,860 + Shewana3_0424 0.72 -0.4 483,872 + Shewana3_0424 0.73 +0.2 483,876 + Shewana3_0424 0.74 +0.3 483,892 - Shewana3_0424 0.76 +0.7 483,960 + Shewana3_0424 0.84 +0.5 484,039 + -0.2 484,039 + +0.0 484,039 + -0.6 484,047 - -0.1 484,165 + +0.4 484,233 + +0.8 484,513 + pdhR Shewana3_0425 0.12 -1.4 484,918 + pdhR Shewana3_0425 0.66 -3.9 485,241 + +3.3 485,271 + +4.1 485,278 + +4.4 485,314 - +2.8 485,325 + +4.5 485,408 + +2.4 485,416 - +4.5 485,800 - aceE Shewana3_0426 0.20 +2.4 485,882 + aceE Shewana3_0426 0.23 +4.5 485,989 - aceE Shewana3_0426 0.27 +2.8 486,036 - aceE Shewana3_0426 0.29 +4.5 486,068 + aceE Shewana3_0426 0.30 +4.8 486,068 + aceE Shewana3_0426 0.30 +4.5
Or see this region's nucleotide sequence