Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tdh and Shew_3711 are separated by 88 nucleotides Shew_3711 and Shew_3712 are separated by 17 nucleotides Shew_3712 and Shew_3713 are separated by 132 nucleotides
Shew_3710: tdh - L-threonine 3-dehydrogenase (RefSeq), at 4,414,940 to 4,415,965
tdh
Shew_3711: Shew_3711 - rhodanese domain-containing protein (RefSeq), at 4,416,054 to 4,416,359
_3711
Shew_3712: Shew_3712 - rhomboid family protein (RefSeq), at 4,416,377 to 4,417,213
_3712
Shew_3713: Shew_3713 - isoprenoid biosynthesis protein (RefSeq), at 4,417,346 to 4,417,999
_3713
Position (kb)
4416
4417 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4415.262 kb on - strand, within tdh at 4415.451 kb on + strand, within tdh at 4415.648 kb on + strand, within tdh at 4415.674 kb on + strand, within tdh at 4415.733 kb on - strand, within tdh at 4415.968 kb on - strand at 4416.299 kb on + strand, within Shew_3711 at 4416.332 kb on + strand at 4416.421 kb on - strand at 4416.433 kb on + strand at 4416.571 kb on + strand, within Shew_3712 at 4416.788 kb on + strand, within Shew_3712 at 4416.902 kb on - strand, within Shew_3712 at 4416.949 kb on - strand, within Shew_3712 at 4417.036 kb on + strand, within Shew_3712 at 4417.161 kb on + strand at 4417.169 kb on - strand at 4417.178 kb on + strand at 4417.205 kb on - strand at 4417.359 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 4,415,262 - tdh Shew_3710 0.31 +3.2 4,415,451 + tdh Shew_3710 0.50 +3.3 4,415,648 + tdh Shew_3710 0.69 +3.4 4,415,674 + tdh Shew_3710 0.72 +3.2 4,415,733 - tdh Shew_3710 0.77 +4.0 4,415,968 - +0.7 4,416,299 + Shew_3711 0.80 -2.1 4,416,332 + -3.0 4,416,421 - +0.2 4,416,433 + +0.5 4,416,571 + Shew_3712 0.23 -0.3 4,416,788 + Shew_3712 0.49 -0.4 4,416,902 - Shew_3712 0.63 -0.1 4,416,949 - Shew_3712 0.68 -0.0 4,417,036 + Shew_3712 0.79 -0.4 4,417,161 + +0.5 4,417,169 - +0.3 4,417,178 + -0.1 4,417,205 - -0.7 4,417,359 + +0.7
Or see this region's nucleotide sequence