Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3326 and Shew_3327 are separated by 78 nucleotides Shew_3327 and Shew_3328 are separated by 476 nucleotides
Shew_3326: Shew_3326 - sodium/hydrogen exchanger (RefSeq), at 3,966,446 to 3,967,687
_3326
Shew_3327: Shew_3327 - RNA-metabolising metallo-beta-lactamase (RefSeq), at 3,967,766 to 3,969,211
_3327
Shew_3328: Shew_3328 - Ig domain-containing protein (RefSeq), at 3,969,688 to 3,972,324
_3328
Position (kb)
3967
3968
3969
3970 Strain fitness (log2 ratio)
-1
0
1 at 3966.949 kb on - strand, within Shew_3326 at 3967.011 kb on - strand, within Shew_3326 at 3967.124 kb on - strand, within Shew_3326 at 3967.275 kb on + strand, within Shew_3326 at 3967.283 kb on - strand, within Shew_3326 at 3967.283 kb on - strand, within Shew_3326 at 3967.810 kb on + strand at 3967.810 kb on + strand at 3967.818 kb on - strand at 3968.035 kb on + strand, within Shew_3327 at 3968.370 kb on - strand, within Shew_3327 at 3968.514 kb on + strand, within Shew_3327 at 3968.514 kb on + strand, within Shew_3327 at 3968.522 kb on - strand, within Shew_3327 at 3968.556 kb on - strand, within Shew_3327 at 3968.605 kb on + strand, within Shew_3327 at 3968.982 kb on - strand, within Shew_3327 at 3969.106 kb on + strand at 3969.269 kb on + strand at 3969.682 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 3,966,949 - Shew_3326 0.40 +0.5 3,967,011 - Shew_3326 0.45 -0.1 3,967,124 - Shew_3326 0.55 +0.9 3,967,275 + Shew_3326 0.67 -0.2 3,967,283 - Shew_3326 0.67 -0.1 3,967,283 - Shew_3326 0.67 -1.2 3,967,810 + +0.0 3,967,810 + -0.1 3,967,818 - -0.0 3,968,035 + Shew_3327 0.19 +1.0 3,968,370 - Shew_3327 0.42 +0.2 3,968,514 + Shew_3327 0.52 +0.5 3,968,514 + Shew_3327 0.52 -0.1 3,968,522 - Shew_3327 0.52 +0.0 3,968,556 - Shew_3327 0.55 +1.3 3,968,605 + Shew_3327 0.58 +0.3 3,968,982 - Shew_3327 0.84 +0.4 3,969,106 + +1.2 3,969,269 + -1.7 3,969,682 + +0.2
Or see this region's nucleotide sequence