Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3313 and Shew_3314 overlap by 4 nucleotides Shew_3314 and Shew_3315 overlap by 8 nucleotides Shew_3315 and Shew_3316 are separated by 100 nucleotides
Shew_3313: Shew_3313 - PTS IIA-like nitrogen-regulatory protein PtsN (RefSeq), at 3,953,401 to 3,953,844
_3313
Shew_3314: Shew_3314 - hypothetical protein (RefSeq), at 3,953,841 to 3,954,689
_3314
Shew_3315: Shew_3315 - phosphotransferase system, phosphocarrier protein HPr (RefSeq), at 3,954,682 to 3,954,957
_3315
Shew_3316: Shew_3316 - magnesium transporter (RefSeq), at 3,955,058 to 3,956,425
_3316
Position (kb) 3954
3955 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 3954.150 kb on - strand, within Shew_3314 at 3954.661 kb on - strand at 3954.696 kb on - strand at 3954.761 kb on - strand, within Shew_3315 at 3955.078 kb on - strand at 3955.217 kb on + strand, within Shew_3316 at 3955.371 kb on - strand, within Shew_3316 at 3955.406 kb on + strand, within Shew_3316 at 3955.793 kb on + strand, within Shew_3316 at 3955.937 kb on - strand, within Shew_3316
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 3,954,150 - Shew_3314 0.36 -1.9 3,954,661 - -3.8 3,954,696 - -0.3 3,954,761 - Shew_3315 0.29 +1.5 3,955,078 - -2.9 3,955,217 + Shew_3316 0.12 -1.8 3,955,371 - Shew_3316 0.23 -0.5 3,955,406 + Shew_3316 0.25 -3.6 3,955,793 + Shew_3316 0.54 -3.1 3,955,937 - Shew_3316 0.64 -2.1
Or see this region's nucleotide sequence