Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2973 and Shew_2974 are separated by 200 nucleotides Shew_2974 and Shew_2975 are separated by 181 nucleotides Shew_2975 and Shew_2976 are separated by 14 nucleotides
Shew_2973: Shew_2973 - endothelin-converting protein 1 (RefSeq), at 3,526,276 to 3,528,360
_2973
Shew_2974: Shew_2974 - hexapaptide repeat-containing transferase (RefSeq), at 3,528,561 to 3,529,103
_2974
Shew_2975: Shew_2975 - pseudouridine synthase (RefSeq), at 3,529,285 to 3,529,977
_2975
Shew_2976: Shew_2976 - hypothetical protein (RefSeq), at 3,529,992 to 3,530,429
_2976
Position (kb)
3528
3529
3530 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3528.329 kb on + strand at 3528.940 kb on + strand, within Shew_2974 at 3529.053 kb on + strand at 3529.124 kb on - strand at 3529.137 kb on + strand at 3529.162 kb on + strand at 3529.173 kb on + strand at 3529.353 kb on - strand at 3529.515 kb on + strand, within Shew_2975 at 3529.698 kb on + strand, within Shew_2975 at 3529.706 kb on - strand, within Shew_2975 at 3529.756 kb on - strand, within Shew_2975 at 3529.961 kb on - strand at 3530.030 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 3,528,329 + -2.5 3,528,940 + Shew_2974 0.70 -0.2 3,529,053 + -0.1 3,529,124 - +1.8 3,529,137 + -0.1 3,529,162 + +1.0 3,529,173 + -0.6 3,529,353 - +0.6 3,529,515 + Shew_2975 0.33 +1.2 3,529,698 + Shew_2975 0.60 -0.1 3,529,706 - Shew_2975 0.61 -1.2 3,529,756 - Shew_2975 0.68 +0.6 3,529,961 - +0.6 3,530,030 - +0.1
Or see this region's nucleotide sequence