Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2551 and Shew_2552 are separated by 73 nucleotides Shew_2552 and Shew_2553 are separated by 118 nucleotides Shew_2553 and Shew_2554 are separated by 80 nucleotides
Shew_2551: Shew_2551 - homoserine O-succinyltransferase (RefSeq), at 3,008,237 to 3,009,202
_2551
Shew_2552: Shew_2552 - hypothetical protein (RefSeq), at 3,009,276 to 3,009,677
_2552
Shew_2553: Shew_2553 - short-chain dehydrogenase/reductase SDR (RefSeq), at 3,009,796 to 3,010,566
_2553
Shew_2554: Shew_2554 - outer membrane protein W (RefSeq), at 3,010,647 to 3,011,288
_2554
Position (kb)
3009
3010 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3008.726 kb on - strand, within Shew_2551 at 3008.795 kb on + strand, within Shew_2551 at 3008.806 kb on + strand, within Shew_2551 at 3008.847 kb on - strand, within Shew_2551 at 3009.230 kb on - strand at 3009.241 kb on - strand at 3009.392 kb on + strand, within Shew_2552 at 3009.427 kb on - strand, within Shew_2552 at 3009.486 kb on + strand, within Shew_2552 at 3009.549 kb on - strand, within Shew_2552 at 3009.549 kb on - strand, within Shew_2552 at 3009.958 kb on - strand, within Shew_2553 at 3010.645 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 3,008,726 - Shew_2551 0.51 -1.5 3,008,795 + Shew_2551 0.58 -1.5 3,008,806 + Shew_2551 0.59 -5.7 3,008,847 - Shew_2551 0.63 -4.2 3,009,230 - -4.7 3,009,241 - -2.1 3,009,392 + Shew_2552 0.29 -5.2 3,009,427 - Shew_2552 0.38 -3.5 3,009,486 + Shew_2552 0.52 -5.1 3,009,549 - Shew_2552 0.68 -4.8 3,009,549 - Shew_2552 0.68 -4.8 3,009,958 - Shew_2553 0.21 +0.5 3,010,645 + -0.2
Or see this region's nucleotide sequence