Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1869 and Shew_1870 are separated by 64 nucleotides Shew_1870 and Shew_1871 overlap by 4 nucleotides Shew_1871 and Shew_1872 are separated by 95 nucleotides
Shew_1869: Shew_1869 - hypothetical protein (RefSeq), at 2,147,862 to 2,148,554
_1869
Shew_1870: Shew_1870 - LacI family transcription regulator (RefSeq), at 2,148,619 to 2,149,647
_1870
Shew_1871: Shew_1871 - DNA polymerase III, epsilon subunit (RefSeq), at 2,149,644 to 2,150,258
_1871
Shew_1872: Shew_1872 - elongation factor G (RefSeq), at 2,150,354 to 2,152,438
_1872
Position (kb)
2148
2149
2150 Strain fitness (log2 ratio)
-1
0
1 at 2147.856 kb on - strand at 2147.858 kb on + strand at 2147.925 kb on + strand at 2147.982 kb on + strand, within Shew_1869 at 2148.129 kb on - strand, within Shew_1869 at 2148.422 kb on + strand, within Shew_1869 at 2148.741 kb on - strand, within Shew_1870 at 2148.811 kb on - strand, within Shew_1870 at 2148.935 kb on - strand, within Shew_1870 at 2148.950 kb on - strand, within Shew_1870 at 2150.213 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 2,147,856 - -0.4 2,147,858 + -0.2 2,147,925 + +0.4 2,147,982 + Shew_1869 0.17 +0.2 2,148,129 - Shew_1869 0.39 +0.3 2,148,422 + Shew_1869 0.81 +0.2 2,148,741 - Shew_1870 0.12 -0.4 2,148,811 - Shew_1870 0.19 +0.9 2,148,935 - Shew_1870 0.31 +0.6 2,148,950 - Shew_1870 0.32 +0.2 2,150,213 + -0.1
Or see this region's nucleotide sequence