Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1809 and Shew_1810 are separated by 62 nucleotides Shew_1810 and Shew_1811 are separated by 159 nucleotides
Shew_1809: Shew_1809 - NAD-glutamate dehydrogenase (RefSeq), at 2,074,877 to 2,079,721
_1809
Shew_1810: Shew_1810 - dihydroorotate dehydrogenase 2 (RefSeq), at 2,079,784 to 2,080,809
_1810
Shew_1811: Shew_1811 - hypothetical protein (RefSeq), at 2,080,969 to 2,081,502
_1811
Position (kb)
2079
2080
2081 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2079.537 kb on + strand at 2080.161 kb on + strand, within Shew_1810 at 2080.658 kb on + strand, within Shew_1810 at 2080.666 kb on - strand, within Shew_1810 at 2080.944 kb on + strand at 2080.952 kb on + strand at 2080.952 kb on + strand at 2080.960 kb on - strand at 2080.960 kb on - strand at 2081.192 kb on + strand, within Shew_1811 at 2081.192 kb on + strand, within Shew_1811 at 2081.192 kb on + strand, within Shew_1811 at 2081.200 kb on - strand, within Shew_1811 at 2081.200 kb on - strand, within Shew_1811 at 2081.200 kb on - strand, within Shew_1811 at 2081.200 kb on - strand, within Shew_1811 at 2081.293 kb on + strand, within Shew_1811 at 2081.438 kb on + strand, within Shew_1811 at 2081.438 kb on + strand, within Shew_1811 at 2081.438 kb on + strand, within Shew_1811 at 2081.499 kb on - strand at 2081.770 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 2,079,537 + +3.7 2,080,161 + Shew_1810 0.37 -5.1 2,080,658 + Shew_1810 0.85 -5.2 2,080,666 - Shew_1810 0.86 -2.9 2,080,944 + +0.8 2,080,952 + -0.7 2,080,952 + -0.2 2,080,960 - -0.0 2,080,960 - +1.8 2,081,192 + Shew_1811 0.42 +0.2 2,081,192 + Shew_1811 0.42 -0.1 2,081,192 + Shew_1811 0.42 +1.1 2,081,200 - Shew_1811 0.43 +0.5 2,081,200 - Shew_1811 0.43 -0.1 2,081,200 - Shew_1811 0.43 +0.4 2,081,200 - Shew_1811 0.43 +0.9 2,081,293 + Shew_1811 0.61 +0.2 2,081,438 + Shew_1811 0.88 +0.2 2,081,438 + Shew_1811 0.88 -2.0 2,081,438 + Shew_1811 0.88 +0.6 2,081,499 - +1.7 2,081,770 - -0.3
Or see this region's nucleotide sequence