Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1297 and guaA are separated by 102 nucleotides guaA and Shew_1299 are separated by 146 nucleotides Shew_1299 and Shew_1300 are separated by 233 nucleotides
Shew_1297: Shew_1297 - inositol-5-monophosphate dehydrogenase (RefSeq), at 1,498,544 to 1,500,010
_1297
Shew_1298: guaA - bifunctional GMP synthase/glutamine amidotransferase protein (RefSeq), at 1,500,113 to 1,501,690
guaA
Shew_1299: Shew_1299 - CMP/dCMP deaminase, zinc-binding (RefSeq), at 1,501,837 to 1,502,373
_1299
Shew_1300: Shew_1300 - lytic transglycosylase, catalytic (RefSeq), at 1,502,607 to 1,504,037
_1300
Position (kb)
1500
1501
1502 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1499.995 kb on - strand at 1500.417 kb on - strand, within guaA at 1500.814 kb on + strand, within guaA at 1500.821 kb on + strand, within guaA at 1501.346 kb on + strand, within guaA at 1501.422 kb on + strand, within guaA at 1501.422 kb on + strand, within guaA at 1501.442 kb on - strand, within guaA at 1501.598 kb on + strand at 1501.791 kb on + strand at 1502.544 kb on - strand at 1502.566 kb on + strand at 1502.566 kb on + strand at 1502.676 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 1,499,995 - -4.2 1,500,417 - guaA Shew_1298 0.19 -3.9 1,500,814 + guaA Shew_1298 0.44 -4.1 1,500,821 + guaA Shew_1298 0.45 -2.0 1,501,346 + guaA Shew_1298 0.78 -3.1 1,501,422 + guaA Shew_1298 0.83 -3.0 1,501,422 + guaA Shew_1298 0.83 -4.4 1,501,442 - guaA Shew_1298 0.84 -2.5 1,501,598 + -3.9 1,501,791 + -0.4 1,502,544 - -0.6 1,502,566 + -1.6 1,502,566 + +0.4 1,502,676 - -0.3
Or see this region's nucleotide sequence