Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1058 and Shew_1059 are separated by 266 nucleotides Shew_1059 and Shew_1060 are separated by 66 nucleotides Shew_1060 and Shew_1061 are separated by 146 nucleotides
Shew_1058: Shew_1058 - holo-acyl-carrier-protein synthase (RefSeq), at 1,220,313 to 1,220,714
_1058
Shew_1059: Shew_1059 - hypothetical protein (RefSeq), at 1,220,981 to 1,221,523
_1059
Shew_1060: Shew_1060 - hypothetical protein (RefSeq), at 1,221,590 to 1,222,873
_1060
Shew_1061: Shew_1061 - cytochrome c assembly protein (RefSeq), at 1,223,020 to 1,223,808
_1061
Position (kb)
1221
1222
1223 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1220.644 kb on + strand, within Shew_1058 at 1221.033 kb on - strand at 1221.293 kb on - strand, within Shew_1059 at 1221.431 kb on - strand, within Shew_1059 at 1221.491 kb on + strand at 1221.602 kb on + strand at 1221.635 kb on - strand at 1221.673 kb on + strand at 1221.713 kb on - strand at 1222.110 kb on + strand, within Shew_1060 at 1222.168 kb on + strand, within Shew_1060 at 1222.481 kb on - strand, within Shew_1060 at 1222.553 kb on + strand, within Shew_1060 at 1222.986 kb on - strand at 1223.012 kb on + strand at 1223.093 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 1,220,644 + Shew_1058 0.82 +4.9 1,221,033 - +0.2 1,221,293 - Shew_1059 0.57 +0.7 1,221,431 - Shew_1059 0.83 +1.2 1,221,491 + +0.6 1,221,602 + -1.1 1,221,635 - -1.8 1,221,673 + -2.7 1,221,713 - -4.3 1,222,110 + Shew_1060 0.40 -4.1 1,222,168 + Shew_1060 0.45 -2.6 1,222,481 - Shew_1060 0.69 -2.4 1,222,553 + Shew_1060 0.75 -3.2 1,222,986 - +0.4 1,223,012 + +0.8 1,223,093 + -2.2
Or see this region's nucleotide sequence