Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0128 and nudE are separated by 97 nucleotides nudE and Shew_0130 are separated by 53 nucleotides Shew_0130 and Shew_0131 overlap by 8 nucleotides Shew_0131 and Shew_0132 are separated by 107 nucleotides
Shew_0128: Shew_0128 - HAD family hydrolase (RefSeq), at 138,259 to 138,933
_0128
Shew_0129: nudE - ADP-ribose diphosphatase NudE (RefSeq), at 139,031 to 139,576
nudE
Shew_0130: Shew_0130 - 3'(2'),5'-bisphosphate nucleotidase (RefSeq), at 139,630 to 140,442
_0130
Shew_0131: Shew_0131 - GCN5-related N-acetyltransferase (RefSeq), at 140,435 to 140,890
_0131
Shew_0132: Shew_0132 - TetR family transcriptional regulator (RefSeq), at 140,998 to 141,654
_0132
Position (kb)
139
140
141 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 138.660 kb on - strand, within Shew_0128 at 138.660 kb on - strand, within Shew_0128 at 138.829 kb on + strand, within Shew_0128 at 138.843 kb on + strand, within Shew_0128 at 139.081 kb on - strand at 139.161 kb on - strand, within nudE at 139.239 kb on + strand, within nudE at 139.310 kb on - strand, within nudE at 139.326 kb on + strand, within nudE at 139.367 kb on - strand, within nudE at 139.488 kb on + strand, within nudE at 139.642 kb on - strand at 139.917 kb on - strand, within Shew_0130 at 140.097 kb on - strand, within Shew_0130 at 140.201 kb on - strand, within Shew_0130 at 140.442 kb on + strand at 140.476 kb on - strand at 140.609 kb on - strand, within Shew_0131 at 140.665 kb on + strand, within Shew_0131 at 140.756 kb on - strand, within Shew_0131 at 140.855 kb on + strand at 141.028 kb on - strand at 141.097 kb on - strand, within Shew_0132 at 141.099 kb on + strand, within Shew_0132 at 141.276 kb on - strand, within Shew_0132 at 141.342 kb on + strand, within Shew_0132
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 138,660 - Shew_0128 0.59 +0.1 138,660 - Shew_0128 0.59 +0.4 138,829 + Shew_0128 0.84 -0.3 138,843 + Shew_0128 0.87 +0.3 139,081 - -0.7 139,161 - nudE Shew_0129 0.24 -0.6 139,239 + nudE Shew_0129 0.38 +0.6 139,310 - nudE Shew_0129 0.51 +1.2 139,326 + nudE Shew_0129 0.54 -0.2 139,367 - nudE Shew_0129 0.62 -1.4 139,488 + nudE Shew_0129 0.84 -0.6 139,642 - -3.0 139,917 - Shew_0130 0.35 -2.3 140,097 - Shew_0130 0.57 -4.3 140,201 - Shew_0130 0.70 -3.6 140,442 + -0.2 140,476 - +0.4 140,609 - Shew_0131 0.38 +0.2 140,665 + Shew_0131 0.50 -0.4 140,756 - Shew_0131 0.70 +0.6 140,855 + -0.1 141,028 - -0.3 141,097 - Shew_0132 0.15 -1.0 141,099 + Shew_0132 0.15 +0.3 141,276 - Shew_0132 0.42 +0.7 141,342 + Shew_0132 0.52 +0.4
Or see this region's nucleotide sequence