Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05185 and MMJJ_RS05190 are separated by 48 nucleotides MMJJ_RS05190 and MMJJ_RS05195 are separated by 38 nucleotides MMJJ_RS05195 and MMJJ_RS05200 are separated by 57 nucleotides MMJJ_RS05200 and MMJJ_RS05205 are separated by 4 nucleotides MMJJ_RS05205 and MMJJ_RS05210 are separated by 50 nucleotides
MMJJ_RS05185: MMJJ_RS05185 - 6,7-dimethyl-8-ribityllumazine synthase, at 958,873 to 959,286
_RS05185
MMJJ_RS05190: MMJJ_RS05190 - gamma carbonic anhydrase family protein, at 959,335 to 959,790
_RS05190
MMJJ_RS05195: MMJJ_RS05195 - hypothetical protein, at 959,829 to 960,101
_RS05195
MMJJ_RS05200: MMJJ_RS05200 - TIGR04013 family B12-binding domain/radical SAM domain-containing protein, at 960,159 to 961,268
_RS05200
MMJJ_RS05205: MMJJ_RS05205 - DUF2118 domain-containing protein, at 961,273 to 961,725
_RS05205
MMJJ_RS05210: MMJJ_RS05210 - polyprenyl synthetase family protein, at 961,776 to 962,729
_RS05210
Position (kb) 960
961
962 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 959.311 kb on + strand at 959.314 kb on + strand at 959.351 kb on + strand at 959.360 kb on - strand at 959.389 kb on - strand, within MMJJ_RS05190 at 959.413 kb on - strand, within MMJJ_RS05190 at 959.445 kb on - strand, within MMJJ_RS05190 at 959.524 kb on - strand, within MMJJ_RS05190 at 959.525 kb on + strand, within MMJJ_RS05190 at 959.551 kb on - strand, within MMJJ_RS05190 at 959.551 kb on - strand, within MMJJ_RS05190 at 959.680 kb on - strand, within MMJJ_RS05190 at 959.691 kb on - strand, within MMJJ_RS05190 at 959.692 kb on + strand, within MMJJ_RS05190 at 959.698 kb on + strand, within MMJJ_RS05190 at 959.711 kb on + strand, within MMJJ_RS05190 at 959.727 kb on - strand, within MMJJ_RS05190 at 959.728 kb on + strand, within MMJJ_RS05190 at 959.791 kb on - strand at 959.792 kb on + strand at 959.793 kb on - strand at 959.802 kb on - strand at 959.802 kb on - strand at 959.815 kb on - strand at 959.901 kb on - strand, within MMJJ_RS05195 at 959.901 kb on - strand, within MMJJ_RS05195 at 959.902 kb on + strand, within MMJJ_RS05195 at 959.993 kb on + strand, within MMJJ_RS05195 at 960.001 kb on - strand, within MMJJ_RS05195 at 960.001 kb on - strand, within MMJJ_RS05195 at 960.006 kb on + strand, within MMJJ_RS05195 at 960.053 kb on - strand, within MMJJ_RS05195 at 960.064 kb on - strand, within MMJJ_RS05195 at 960.077 kb on - strand at 960.077 kb on - strand at 960.079 kb on - strand at 960.079 kb on - strand at 960.080 kb on + strand at 960.081 kb on - strand at 960.081 kb on - strand at 960.081 kb on - strand at 960.081 kb on - strand at 960.081 kb on - strand at 960.081 kb on - strand at 960.081 kb on - strand at 960.082 kb on + strand at 960.082 kb on + strand at 960.082 kb on + strand at 960.082 kb on + strand at 960.082 kb on + strand at 960.082 kb on + strand at 960.082 kb on + strand at 960.131 kb on - strand at 960.131 kb on - strand at 960.132 kb on + strand at 960.133 kb on - strand at 960.134 kb on + strand at 960.135 kb on - strand at 960.135 kb on - strand at 960.136 kb on + strand at 960.136 kb on + strand at 960.136 kb on + strand at 960.148 kb on - strand at 960.148 kb on - strand at 960.148 kb on - strand at 960.148 kb on - strand at 960.148 kb on - strand at 960.148 kb on - strand at 960.149 kb on + strand at 960.149 kb on + strand at 960.152 kb on - strand at 960.152 kb on - strand at 960.158 kb on - strand at 960.170 kb on - strand at 960.192 kb on - strand at 960.192 kb on - strand at 960.192 kb on - strand at 960.193 kb on + strand at 960.193 kb on + strand at 960.193 kb on + strand at 960.198 kb on + strand at 960.198 kb on + strand at 960.198 kb on + strand at 960.208 kb on - strand at 960.209 kb on + strand at 960.209 kb on + strand at 960.209 kb on + strand at 960.210 kb on - strand at 960.217 kb on - strand at 960.221 kb on + strand at 960.221 kb on + strand at 960.232 kb on - strand at 960.232 kb on - strand at 960.283 kb on - strand, within MMJJ_RS05200 at 960.289 kb on + strand, within MMJJ_RS05200 at 960.302 kb on - strand, within MMJJ_RS05200 at 960.302 kb on - strand, within MMJJ_RS05200 at 960.331 kb on - strand, within MMJJ_RS05200 at 960.332 kb on + strand, within MMJJ_RS05200 at 960.362 kb on - strand, within MMJJ_RS05200 at 960.362 kb on - strand, within MMJJ_RS05200 at 960.364 kb on - strand, within MMJJ_RS05200 at 960.364 kb on - strand, within MMJJ_RS05200 at 960.408 kb on + strand, within MMJJ_RS05200 at 960.433 kb on - strand, within MMJJ_RS05200 at 960.436 kb on - strand, within MMJJ_RS05200 at 960.473 kb on + strand, within MMJJ_RS05200 at 960.512 kb on - strand, within MMJJ_RS05200 at 960.579 kb on + strand, within MMJJ_RS05200 at 960.594 kb on - strand, within MMJJ_RS05200 at 960.594 kb on - strand, within MMJJ_RS05200 at 960.594 kb on - strand, within MMJJ_RS05200 at 960.594 kb on - strand, within MMJJ_RS05200 at 960.602 kb on - strand at 960.603 kb on + strand, within MMJJ_RS05200 at 960.626 kb on + strand, within MMJJ_RS05200 at 960.626 kb on + strand, within MMJJ_RS05200 at 960.626 kb on + strand, within MMJJ_RS05200 at 960.638 kb on - strand, within MMJJ_RS05200 at 960.641 kb on + strand, within MMJJ_RS05200 at 960.647 kb on - strand, within MMJJ_RS05200 at 960.647 kb on - strand, within MMJJ_RS05200 at 960.647 kb on - strand, within MMJJ_RS05200 at 960.648 kb on + strand, within MMJJ_RS05200 at 960.648 kb on + strand, within MMJJ_RS05200 at 960.654 kb on + strand, within MMJJ_RS05200 at 960.654 kb on + strand, within MMJJ_RS05200 at 960.663 kb on + strand, within MMJJ_RS05200 at 960.703 kb on - strand, within MMJJ_RS05200 at 960.723 kb on - strand, within MMJJ_RS05200 at 960.724 kb on + strand, within MMJJ_RS05200 at 960.753 kb on + strand, within MMJJ_RS05200 at 960.832 kb on - strand, within MMJJ_RS05200 at 960.833 kb on + strand, within MMJJ_RS05200 at 960.869 kb on + strand, within MMJJ_RS05200 at 960.869 kb on + strand, within MMJJ_RS05200 at 960.875 kb on - strand, within MMJJ_RS05200 at 960.875 kb on - strand, within MMJJ_RS05200 at 960.894 kb on - strand, within MMJJ_RS05200 at 960.941 kb on - strand, within MMJJ_RS05200 at 960.941 kb on - strand, within MMJJ_RS05200 at 960.942 kb on + strand, within MMJJ_RS05200 at 960.942 kb on + strand, within MMJJ_RS05200 at 960.942 kb on + strand, within MMJJ_RS05200 at 960.943 kb on - strand, within MMJJ_RS05200 at 960.943 kb on - strand, within MMJJ_RS05200 at 960.944 kb on + strand, within MMJJ_RS05200 at 961.007 kb on + strand, within MMJJ_RS05200 at 961.026 kb on + strand, within MMJJ_RS05200 at 961.086 kb on - strand, within MMJJ_RS05200 at 961.086 kb on - strand, within MMJJ_RS05200 at 961.087 kb on + strand, within MMJJ_RS05200 at 961.091 kb on - strand, within MMJJ_RS05200 at 961.091 kb on - strand, within MMJJ_RS05200 at 961.093 kb on - strand, within MMJJ_RS05200 at 961.122 kb on - strand, within MMJJ_RS05200 at 961.123 kb on + strand, within MMJJ_RS05200 at 961.123 kb on + strand, within MMJJ_RS05200 at 961.127 kb on - strand, within MMJJ_RS05200 at 961.128 kb on + strand, within MMJJ_RS05200 at 961.288 kb on + strand at 961.289 kb on - strand at 961.289 kb on - strand at 961.290 kb on + strand at 961.290 kb on + strand at 961.299 kb on + strand at 961.333 kb on - strand, within MMJJ_RS05205 at 961.333 kb on - strand, within MMJJ_RS05205 at 961.359 kb on - strand, within MMJJ_RS05205 at 961.370 kb on + strand, within MMJJ_RS05205 at 961.483 kb on + strand, within MMJJ_RS05205 at 961.499 kb on - strand, within MMJJ_RS05205 at 961.499 kb on - strand, within MMJJ_RS05205 at 961.499 kb on - strand, within MMJJ_RS05205 at 961.499 kb on - strand, within MMJJ_RS05205 at 961.499 kb on - strand, within MMJJ_RS05205 at 961.499 kb on - strand, within MMJJ_RS05205 at 961.500 kb on + strand, within MMJJ_RS05205 at 961.500 kb on + strand, within MMJJ_RS05205 at 961.503 kb on - strand, within MMJJ_RS05205 at 961.503 kb on - strand, within MMJJ_RS05205 at 961.503 kb on - strand, within MMJJ_RS05205 at 961.503 kb on - strand, within MMJJ_RS05205 at 961.504 kb on + strand, within MMJJ_RS05205 at 961.504 kb on + strand, within MMJJ_RS05205 at 961.504 kb on + strand, within MMJJ_RS05205 at 961.504 kb on + strand, within MMJJ_RS05205 at 961.504 kb on + strand, within MMJJ_RS05205 at 961.504 kb on + strand, within MMJJ_RS05205 at 961.504 kb on + strand, within MMJJ_RS05205 at 961.509 kb on - strand, within MMJJ_RS05205 at 961.510 kb on + strand, within MMJJ_RS05205 at 961.510 kb on + strand, within MMJJ_RS05205 at 961.510 kb on + strand, within MMJJ_RS05205 at 961.531 kb on - strand, within MMJJ_RS05205 at 961.532 kb on + strand, within MMJJ_RS05205 at 961.532 kb on + strand, within MMJJ_RS05205 at 961.556 kb on + strand, within MMJJ_RS05205 at 961.565 kb on - strand, within MMJJ_RS05205 at 961.566 kb on + strand, within MMJJ_RS05205 at 961.566 kb on + strand, within MMJJ_RS05205 at 961.574 kb on + strand, within MMJJ_RS05205 at 961.583 kb on - strand, within MMJJ_RS05205 at 961.583 kb on - strand, within MMJJ_RS05205 at 961.583 kb on - strand, within MMJJ_RS05205 at 961.583 kb on - strand, within MMJJ_RS05205 at 961.583 kb on - strand, within MMJJ_RS05205 at 961.584 kb on + strand, within MMJJ_RS05205 at 961.584 kb on + strand, within MMJJ_RS05205 at 961.585 kb on - strand, within MMJJ_RS05205 at 961.586 kb on + strand, within MMJJ_RS05205 at 961.586 kb on + strand, within MMJJ_RS05205 at 961.586 kb on + strand, within MMJJ_RS05205 at 961.586 kb on + strand, within MMJJ_RS05205 at 961.586 kb on + strand, within MMJJ_RS05205 at 961.589 kb on + strand, within MMJJ_RS05205 at 961.589 kb on + strand, within MMJJ_RS05205 at 961.589 kb on + strand, within MMJJ_RS05205 at 961.589 kb on + strand, within MMJJ_RS05205 at 961.691 kb on + strand at 961.706 kb on - strand at 961.737 kb on - strand at 961.763 kb on - strand at 961.764 kb on + strand at 961.767 kb on - strand at 961.768 kb on + strand at 962.146 kb on - strand, within MMJJ_RS05210
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE remove 959,311 + -1.9 959,314 + -2.6 959,351 + -1.5 959,360 - -3.3 959,389 - MMJJ_RS05190 0.12 +1.0 959,413 - MMJJ_RS05190 0.17 +1.2 959,445 - MMJJ_RS05190 0.24 +0.4 959,524 - MMJJ_RS05190 0.41 +0.8 959,525 + MMJJ_RS05190 0.42 -0.9 959,551 - MMJJ_RS05190 0.47 -2.0 959,551 - MMJJ_RS05190 0.47 -1.3 959,680 - MMJJ_RS05190 0.76 +0.7 959,691 - MMJJ_RS05190 0.78 -2.2 959,692 + MMJJ_RS05190 0.78 -0.0 959,698 + MMJJ_RS05190 0.80 -0.2 959,711 + MMJJ_RS05190 0.82 -1.9 959,727 - MMJJ_RS05190 0.86 +1.1 959,728 + MMJJ_RS05190 0.86 +0.3 959,791 - +1.3 959,792 + -1.9 959,793 - -1.7 959,802 - +0.0 959,802 - +1.4 959,815 - -1.7 959,901 - MMJJ_RS05195 0.26 -1.5 959,901 - MMJJ_RS05195 0.26 -0.2 959,902 + MMJJ_RS05195 0.27 +1.1 959,993 + MMJJ_RS05195 0.60 -1.3 960,001 - MMJJ_RS05195 0.63 +0.6 960,001 - MMJJ_RS05195 0.63 +0.7 960,006 + MMJJ_RS05195 0.65 -1.7 960,053 - MMJJ_RS05195 0.82 +1.2 960,064 - MMJJ_RS05195 0.86 -2.4 960,077 - +0.3 960,077 - -1.0 960,079 - +0.8 960,079 - -1.6 960,080 + +2.6 960,081 - +2.3 960,081 - -0.1 960,081 - -1.1 960,081 - -1.3 960,081 - +0.0 960,081 - -1.5 960,081 - +0.7 960,082 + +0.3 960,082 + +0.1 960,082 + +1.2 960,082 + +0.9 960,082 + +1.2 960,082 + -1.6 960,082 + +0.7 960,131 - +0.6 960,131 - -2.0 960,132 + +0.4 960,133 - +0.4 960,134 + -2.0 960,135 - +0.6 960,135 - -1.7 960,136 + +0.9 960,136 + +0.4 960,136 + +0.4 960,148 - +0.5 960,148 - -1.6 960,148 - +0.9 960,148 - -1.8 960,148 - +0.1 960,148 - -0.9 960,149 + +1.0 960,149 + +1.1 960,152 - +0.6 960,152 - -1.3 960,158 - -1.5 960,170 - +0.6 960,192 - -0.5 960,192 - +0.9 960,192 - +1.2 960,193 + -1.3 960,193 + +1.2 960,193 + -2.9 960,198 + -0.5 960,198 + -1.0 960,198 + +0.5 960,208 - -1.4 960,209 + +0.9 960,209 + +0.6 960,209 + -0.3 960,210 - +0.5 960,217 - +2.2 960,221 + +0.2 960,221 + +0.7 960,232 - +1.3 960,232 - -1.5 960,283 - MMJJ_RS05200 0.11 -2.0 960,289 + MMJJ_RS05200 0.12 +1.0 960,302 - MMJJ_RS05200 0.13 +1.4 960,302 - MMJJ_RS05200 0.13 +0.9 960,331 - MMJJ_RS05200 0.15 -2.6 960,332 + MMJJ_RS05200 0.16 +2.2 960,362 - MMJJ_RS05200 0.18 -1.2 960,362 - MMJJ_RS05200 0.18 +0.8 960,364 - MMJJ_RS05200 0.18 -1.2 960,364 - MMJJ_RS05200 0.18 -1.2 960,408 + MMJJ_RS05200 0.22 -1.9 960,433 - MMJJ_RS05200 0.25 -1.6 960,436 - MMJJ_RS05200 0.25 -2.4 960,473 + MMJJ_RS05200 0.28 +0.3 960,512 - MMJJ_RS05200 0.32 +1.4 960,579 + MMJJ_RS05200 0.38 -0.7 960,594 - MMJJ_RS05200 0.39 +1.6 960,594 - MMJJ_RS05200 0.39 -0.3 960,594 - MMJJ_RS05200 0.39 -2.1 960,594 - MMJJ_RS05200 0.39 -0.5 960,602 - +1.3 960,603 + MMJJ_RS05200 0.40 -1.9 960,626 + MMJJ_RS05200 0.42 +0.6 960,626 + MMJJ_RS05200 0.42 -1.7 960,626 + MMJJ_RS05200 0.42 +0.5 960,638 - MMJJ_RS05200 0.43 +1.2 960,641 + MMJJ_RS05200 0.43 -2.0 960,647 - MMJJ_RS05200 0.44 -1.5 960,647 - MMJJ_RS05200 0.44 +1.2 960,647 - MMJJ_RS05200 0.44 -0.8 960,648 + MMJJ_RS05200 0.44 -1.3 960,648 + MMJJ_RS05200 0.44 +0.6 960,654 + MMJJ_RS05200 0.45 -1.4 960,654 + MMJJ_RS05200 0.45 -0.1 960,663 + MMJJ_RS05200 0.45 -2.0 960,703 - MMJJ_RS05200 0.49 -1.9 960,723 - MMJJ_RS05200 0.51 -1.7 960,724 + MMJJ_RS05200 0.51 -0.1 960,753 + MMJJ_RS05200 0.54 +1.3 960,832 - MMJJ_RS05200 0.61 +0.9 960,833 + MMJJ_RS05200 0.61 -1.7 960,869 + MMJJ_RS05200 0.64 +0.5 960,869 + MMJJ_RS05200 0.64 -0.0 960,875 - MMJJ_RS05200 0.65 +0.4 960,875 - MMJJ_RS05200 0.65 -1.3 960,894 - MMJJ_RS05200 0.66 +1.2 960,941 - MMJJ_RS05200 0.70 +0.7 960,941 - MMJJ_RS05200 0.70 -2.3 960,942 + MMJJ_RS05200 0.71 +0.8 960,942 + MMJJ_RS05200 0.71 -1.4 960,942 + MMJJ_RS05200 0.71 -3.5 960,943 - MMJJ_RS05200 0.71 -1.2 960,943 - MMJJ_RS05200 0.71 -1.8 960,944 + MMJJ_RS05200 0.71 -3.4 961,007 + MMJJ_RS05200 0.76 -0.5 961,026 + MMJJ_RS05200 0.78 -1.6 961,086 - MMJJ_RS05200 0.84 +1.9 961,086 - MMJJ_RS05200 0.84 +0.3 961,087 + MMJJ_RS05200 0.84 +0.0 961,091 - MMJJ_RS05200 0.84 +0.5 961,091 - MMJJ_RS05200 0.84 -1.6 961,093 - MMJJ_RS05200 0.84 +0.7 961,122 - MMJJ_RS05200 0.87 +0.7 961,123 + MMJJ_RS05200 0.87 +0.0 961,123 + MMJJ_RS05200 0.87 -0.9 961,127 - MMJJ_RS05200 0.87 -1.8 961,128 + MMJJ_RS05200 0.87 -1.5 961,288 + +0.6 961,289 - -1.6 961,289 - +1.1 961,290 + +0.6 961,290 + -1.5 961,299 + +0.7 961,333 - MMJJ_RS05205 0.13 -0.7 961,333 - MMJJ_RS05205 0.13 +0.6 961,359 - MMJJ_RS05205 0.19 +0.8 961,370 + MMJJ_RS05205 0.21 -1.4 961,483 + MMJJ_RS05205 0.46 -0.3 961,499 - MMJJ_RS05205 0.50 -3.8 961,499 - MMJJ_RS05205 0.50 +0.9 961,499 - MMJJ_RS05205 0.50 -0.7 961,499 - MMJJ_RS05205 0.50 -0.7 961,499 - MMJJ_RS05205 0.50 -4.4 961,499 - MMJJ_RS05205 0.50 +1.7 961,500 + MMJJ_RS05205 0.50 -1.8 961,500 + MMJJ_RS05205 0.50 +2.2 961,503 - MMJJ_RS05205 0.51 +1.6 961,503 - MMJJ_RS05205 0.51 +0.6 961,503 - MMJJ_RS05205 0.51 -0.1 961,503 - MMJJ_RS05205 0.51 -0.4 961,504 + MMJJ_RS05205 0.51 -1.7 961,504 + MMJJ_RS05205 0.51 +1.0 961,504 + MMJJ_RS05205 0.51 -2.2 961,504 + MMJJ_RS05205 0.51 -1.3 961,504 + MMJJ_RS05205 0.51 -1.5 961,504 + MMJJ_RS05205 0.51 -2.1 961,504 + MMJJ_RS05205 0.51 -0.6 961,509 - MMJJ_RS05205 0.52 -1.7 961,510 + MMJJ_RS05205 0.52 +0.3 961,510 + MMJJ_RS05205 0.52 +1.0 961,510 + MMJJ_RS05205 0.52 -1.1 961,531 - MMJJ_RS05205 0.57 -0.7 961,532 + MMJJ_RS05205 0.57 +0.7 961,532 + MMJJ_RS05205 0.57 +0.9 961,556 + MMJJ_RS05205 0.62 +0.4 961,565 - MMJJ_RS05205 0.64 -1.2 961,566 + MMJJ_RS05205 0.65 +0.7 961,566 + MMJJ_RS05205 0.65 +1.8 961,574 + MMJJ_RS05205 0.66 +0.4 961,583 - MMJJ_RS05205 0.68 +0.5 961,583 - MMJJ_RS05205 0.68 +1.4 961,583 - MMJJ_RS05205 0.68 +0.7 961,583 - MMJJ_RS05205 0.68 +0.9 961,583 - MMJJ_RS05205 0.68 -1.0 961,584 + MMJJ_RS05205 0.69 -0.0 961,584 + MMJJ_RS05205 0.69 +0.8 961,585 - MMJJ_RS05205 0.69 +0.1 961,586 + MMJJ_RS05205 0.69 +0.9 961,586 + MMJJ_RS05205 0.69 +1.0 961,586 + MMJJ_RS05205 0.69 +0.9 961,586 + MMJJ_RS05205 0.69 -1.9 961,586 + MMJJ_RS05205 0.69 -1.9 961,589 + MMJJ_RS05205 0.70 -0.0 961,589 + MMJJ_RS05205 0.70 +1.0 961,589 + MMJJ_RS05205 0.70 +0.2 961,589 + MMJJ_RS05205 0.70 +0.9 961,691 + +0.8 961,706 - -0.8 961,737 - +0.6 961,763 - +1.2 961,764 + +0.3 961,767 - -0.3 961,768 + -1.5 962,146 - MMJJ_RS05210 0.39 +0.2
Or see this region's nucleotide sequence