Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03235 and MMJJ_RS03240 are separated by 289 nucleotides MMJJ_RS03240 and MMJJ_RS03245 are separated by 236 nucleotides MMJJ_RS03245 and MMJJ_RS03250 are separated by 154 nucleotides MMJJ_RS03250 and MMJJ_RS03255 are separated by 172 nucleotides MMJJ_RS03255 and MMJJ_RS03260 are separated by 99 nucleotides
MMJJ_RS03235: MMJJ_RS03235 - hypothetical protein, at 612,743 to 613,264
_RS03235
MMJJ_RS03240: MMJJ_RS03240 - histone family protein, at 613,554 to 613,757
_RS03240
MMJJ_RS03245: MMJJ_RS03245 - UMP kinase, at 613,994 to 614,671
_RS03245
MMJJ_RS03250: MMJJ_RS03250 - DUF2116 family Zn-ribbon domain-containing protein, at 614,826 to 615,008
_RS03250
MMJJ_RS03255: MMJJ_RS03255 - tRNA-Met, at 615,181 to 615,259
_RS03255
MMJJ_RS03260: MMJJ_RS03260 - tRNA-Met, at 615,359 to 615,437
_RS03260
Position (kb) 613
614
615 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 613.056 kb on - strand, within MMJJ_RS03235 at 613.348 kb on - strand at 613.400 kb on + strand at 613.403 kb on + strand at 613.405 kb on - strand at 613.406 kb on + strand at 613.408 kb on - strand at 613.410 kb on - strand at 613.410 kb on - strand at 613.410 kb on - strand at 613.411 kb on + strand at 613.411 kb on + strand at 613.415 kb on + strand at 613.415 kb on + strand at 613.420 kb on + strand at 613.422 kb on - strand at 613.425 kb on + strand at 613.425 kb on + strand at 613.429 kb on - strand at 613.430 kb on + strand at 613.430 kb on + strand at 613.449 kb on + strand at 613.449 kb on + strand at 613.469 kb on + strand at 613.507 kb on + strand at 613.507 kb on + strand at 613.507 kb on + strand at 613.553 kb on - strand at 613.565 kb on + strand at 613.582 kb on - strand, within MMJJ_RS03240 at 613.588 kb on - strand, within MMJJ_RS03240 at 613.616 kb on + strand, within MMJJ_RS03240 at 613.756 kb on + strand at 613.766 kb on - strand at 613.767 kb on + strand at 613.845 kb on - strand at 613.846 kb on + strand at 613.858 kb on - strand at 613.859 kb on + strand at 613.864 kb on + strand at 613.864 kb on + strand at 613.917 kb on + strand at 613.930 kb on + strand at 614.002 kb on + strand at 614.150 kb on - strand, within MMJJ_RS03245 at 614.432 kb on - strand, within MMJJ_RS03245 at 614.663 kb on + strand at 614.686 kb on + strand at 614.773 kb on - strand at 614.777 kb on - strand at 614.777 kb on - strand at 614.804 kb on - strand at 614.805 kb on + strand at 614.805 kb on + strand at 614.807 kb on - strand at 614.807 kb on - strand at 614.848 kb on - strand, within MMJJ_RS03250 at 614.848 kb on - strand, within MMJJ_RS03250 at 614.848 kb on - strand, within MMJJ_RS03250 at 614.992 kb on + strand at 615.042 kb on + strand at 615.058 kb on - strand at 615.265 kb on - strand at 615.266 kb on + strand at 615.343 kb on - strand at 615.346 kb on - strand at 615.346 kb on - strand at 615.346 kb on - strand at 615.347 kb on + strand at 615.459 kb on - strand at 615.459 kb on - strand at 615.498 kb on + strand at 615.498 kb on + strand at 615.567 kb on - strand at 615.569 kb on - strand at 615.637 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE remove 613,056 - MMJJ_RS03235 0.60 -2.7 613,348 - +0.2 613,400 + +2.3 613,403 + -2.3 613,405 - -2.5 613,406 + +0.6 613,408 - -1.6 613,410 - +0.1 613,410 - -1.0 613,410 - -2.9 613,411 + +0.2 613,411 + -2.2 613,415 + -0.2 613,415 + -0.8 613,420 + -1.6 613,422 - +0.5 613,425 + -1.2 613,425 + -1.7 613,429 - -2.3 613,430 + +0.3 613,430 + +0.6 613,449 + +0.2 613,449 + +0.9 613,469 + -0.3 613,507 + -0.4 613,507 + -1.2 613,507 + -3.0 613,553 - -0.0 613,565 + -0.5 613,582 - MMJJ_RS03240 0.14 +0.2 613,588 - MMJJ_RS03240 0.17 -2.7 613,616 + MMJJ_RS03240 0.30 -1.3 613,756 + -2.0 613,766 - +1.5 613,767 + -0.8 613,845 - +0.2 613,846 + +0.2 613,858 - -1.3 613,859 + -1.9 613,864 + +0.7 613,864 + +0.7 613,917 + -1.6 613,930 + +0.2 614,002 + -1.6 614,150 - MMJJ_RS03245 0.23 -3.0 614,432 - MMJJ_RS03245 0.65 -4.3 614,663 + +0.9 614,686 + +1.5 614,773 - -1.4 614,777 - +1.1 614,777 - +0.1 614,804 - +0.2 614,805 + +0.7 614,805 + +0.8 614,807 - -0.4 614,807 - -0.1 614,848 - MMJJ_RS03250 0.12 -0.0 614,848 - MMJJ_RS03250 0.12 +0.6 614,848 - MMJJ_RS03250 0.12 -1.1 614,992 + -2.7 615,042 + +0.4 615,058 - -1.2 615,265 - -1.0 615,266 + -1.1 615,343 - -0.2 615,346 - -0.0 615,346 - +0.7 615,346 - +1.3 615,347 + +0.2 615,459 - +0.8 615,459 - -1.5 615,498 + -1.8 615,498 + -2.2 615,567 - -2.4 615,569 - +0.4 615,637 + +1.2
Or see this region's nucleotide sequence