Experiment: R2A with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22555 and CA265_RS22560 are separated by 11 nucleotides CA265_RS22560 and CA265_RS22565 are separated by 71 nucleotides CA265_RS22565 and CA265_RS22570 are separated by 733 nucleotides
CA265_RS22555: CA265_RS22555 - lipoyl(octanoyl) transferase, at 5,527,030 to 5,527,758
_RS22555
CA265_RS22560: CA265_RS22560 - 4'-phosphopantetheinyl transferase, at 5,527,770 to 5,528,417
_RS22560
CA265_RS22565: CA265_RS22565 - dCTP deaminase, at 5,528,489 to 5,529,025
_RS22565
CA265_RS22570: CA265_RS22570 - TonB-dependent receptor, at 5,529,759 to 5,532,215
_RS22570
Position (kb)
5528
5529
5530 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5527.767 kb on - strand at 5527.785 kb on + strand at 5527.785 kb on + strand at 5527.795 kb on - strand at 5528.434 kb on + strand at 5528.455 kb on + strand at 5528.463 kb on - strand at 5528.569 kb on - strand, within CA265_RS22565 at 5528.603 kb on + strand, within CA265_RS22565 at 5528.611 kb on + strand, within CA265_RS22565 at 5528.617 kb on + strand, within CA265_RS22565 at 5528.618 kb on - strand, within CA265_RS22565 at 5528.675 kb on - strand, within CA265_RS22565 at 5528.685 kb on - strand, within CA265_RS22565 at 5528.685 kb on - strand, within CA265_RS22565 at 5528.686 kb on + strand, within CA265_RS22565 at 5528.686 kb on + strand, within CA265_RS22565 at 5528.686 kb on + strand, within CA265_RS22565 at 5528.686 kb on + strand, within CA265_RS22565 at 5528.687 kb on - strand, within CA265_RS22565 at 5528.707 kb on - strand, within CA265_RS22565 at 5528.724 kb on + strand, within CA265_RS22565 at 5528.729 kb on + strand, within CA265_RS22565 at 5528.751 kb on + strand, within CA265_RS22565 at 5528.767 kb on + strand, within CA265_RS22565 at 5528.767 kb on + strand, within CA265_RS22565 at 5528.776 kb on + strand, within CA265_RS22565 at 5528.815 kb on + strand, within CA265_RS22565 at 5528.837 kb on - strand, within CA265_RS22565 at 5528.945 kb on + strand, within CA265_RS22565 at 5528.946 kb on - strand, within CA265_RS22565 at 5528.965 kb on + strand, within CA265_RS22565 at 5528.965 kb on + strand, within CA265_RS22565 at 5528.965 kb on + strand, within CA265_RS22565 at 5528.966 kb on - strand, within CA265_RS22565 at 5528.969 kb on + strand, within CA265_RS22565 at 5528.977 kb on + strand at 5528.978 kb on - strand at 5529.017 kb on - strand at 5529.085 kb on + strand at 5529.086 kb on - strand at 5529.106 kb on + strand at 5529.156 kb on - strand at 5529.220 kb on + strand at 5529.220 kb on + strand at 5529.331 kb on + strand at 5529.332 kb on - strand at 5529.332 kb on - strand at 5529.476 kb on - strand at 5529.482 kb on + strand at 5529.482 kb on + strand at 5529.507 kb on + strand at 5529.508 kb on - strand at 5529.518 kb on + strand at 5529.518 kb on + strand at 5529.518 kb on + strand at 5529.519 kb on - strand at 5529.619 kb on + strand at 5529.644 kb on + strand at 5529.687 kb on + strand at 5529.688 kb on - strand at 5529.707 kb on + strand at 5529.731 kb on - strand at 5529.774 kb on + strand at 5529.774 kb on + strand at 5529.774 kb on + strand at 5529.775 kb on - strand at 5529.819 kb on - strand at 5529.843 kb on - strand at 5529.885 kb on - strand at 5529.888 kb on + strand at 5529.896 kb on + strand at 5529.903 kb on + strand at 5529.904 kb on - strand at 5529.906 kb on - strand at 5529.913 kb on - strand at 5529.943 kb on - strand at 5529.943 kb on - strand at 5529.978 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.025 mg/ml remove 5,527,767 - +0.1 5,527,785 + +0.5 5,527,785 + -0.0 5,527,795 - +0.8 5,528,434 + +0.4 5,528,455 + +0.2 5,528,463 - +0.3 5,528,569 - CA265_RS22565 0.15 +0.6 5,528,603 + CA265_RS22565 0.21 -1.6 5,528,611 + CA265_RS22565 0.23 +0.4 5,528,617 + CA265_RS22565 0.24 -0.1 5,528,618 - CA265_RS22565 0.24 +0.6 5,528,675 - CA265_RS22565 0.35 +0.1 5,528,685 - CA265_RS22565 0.36 +0.1 5,528,685 - CA265_RS22565 0.36 +0.1 5,528,686 + CA265_RS22565 0.37 -0.2 5,528,686 + CA265_RS22565 0.37 +0.3 5,528,686 + CA265_RS22565 0.37 +0.7 5,528,686 + CA265_RS22565 0.37 +0.7 5,528,687 - CA265_RS22565 0.37 -2.3 5,528,707 - CA265_RS22565 0.41 -0.0 5,528,724 + CA265_RS22565 0.44 +1.0 5,528,729 + CA265_RS22565 0.45 -1.0 5,528,751 + CA265_RS22565 0.49 -1.4 5,528,767 + CA265_RS22565 0.52 +0.4 5,528,767 + CA265_RS22565 0.52 +1.2 5,528,776 + CA265_RS22565 0.53 +1.0 5,528,815 + CA265_RS22565 0.61 -0.8 5,528,837 - CA265_RS22565 0.65 +0.8 5,528,945 + CA265_RS22565 0.85 +0.5 5,528,946 - CA265_RS22565 0.85 +1.6 5,528,965 + CA265_RS22565 0.89 +0.4 5,528,965 + CA265_RS22565 0.89 -0.1 5,528,965 + CA265_RS22565 0.89 +1.0 5,528,966 - CA265_RS22565 0.89 +1.0 5,528,969 + CA265_RS22565 0.89 +0.6 5,528,977 + -0.7 5,528,978 - -1.1 5,529,017 - +0.3 5,529,085 + -0.1 5,529,086 - -0.2 5,529,106 + +2.0 5,529,156 - +0.1 5,529,220 + -0.1 5,529,220 + +0.2 5,529,331 + -1.2 5,529,332 - +1.6 5,529,332 - -0.4 5,529,476 - +0.1 5,529,482 + +0.8 5,529,482 + -1.0 5,529,507 + -0.3 5,529,508 - -1.1 5,529,518 + -1.1 5,529,518 + +0.1 5,529,518 + +0.6 5,529,519 - -0.7 5,529,619 + -2.1 5,529,644 + -0.4 5,529,687 + +0.3 5,529,688 - +0.2 5,529,707 + +1.1 5,529,731 - +1.0 5,529,774 + -0.0 5,529,774 + +0.2 5,529,774 + +0.9 5,529,775 - +0.6 5,529,819 - +0.7 5,529,843 - -0.1 5,529,885 - +0.0 5,529,888 + -0.3 5,529,896 + -0.0 5,529,903 + +0.7 5,529,904 - -1.5 5,529,906 - +0.7 5,529,913 - +0.8 5,529,943 - +0.1 5,529,943 - -1.2 5,529,978 - -1.0
Or see this region's nucleotide sequence