Experiment: R2A with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15965 and CA265_RS15970 are separated by 139 nucleotides CA265_RS15970 and CA265_RS15975 are separated by 246 nucleotides CA265_RS15975 and CA265_RS15980 are separated by 27 nucleotides CA265_RS15980 and CA265_RS15985 are separated by 11 nucleotides
CA265_RS15965: CA265_RS15965 - glutamyl-tRNA reductase, at 4,008,577 to 4,009,803
_RS15965
CA265_RS15970: CA265_RS15970 - DoxX family protein, at 4,009,943 to 4,010,323
_RS15970
CA265_RS15975: CA265_RS15975 - alpha/beta hydrolase, at 4,010,570 to 4,011,397
_RS15975
CA265_RS15980: CA265_RS15980 - hypothetical protein, at 4,011,425 to 4,011,958
_RS15980
CA265_RS15985: CA265_RS15985 - mechanosensitive ion channel protein MscS, at 4,011,970 to 4,013,244
_RS15985
Position (kb)
4010
4011
4012 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4009.960 kb on + strand at 4010.008 kb on + strand, within CA265_RS15970 at 4010.008 kb on + strand, within CA265_RS15970 at 4010.031 kb on + strand, within CA265_RS15970 at 4010.034 kb on - strand, within CA265_RS15970 at 4010.037 kb on - strand, within CA265_RS15970 at 4010.040 kb on + strand, within CA265_RS15970 at 4010.040 kb on + strand, within CA265_RS15970 at 4010.040 kb on + strand, within CA265_RS15970 at 4010.044 kb on + strand, within CA265_RS15970 at 4010.118 kb on + strand, within CA265_RS15970 at 4010.128 kb on + strand, within CA265_RS15970 at 4010.161 kb on + strand, within CA265_RS15970 at 4010.161 kb on + strand, within CA265_RS15970 at 4010.161 kb on + strand, within CA265_RS15970 at 4010.195 kb on - strand, within CA265_RS15970 at 4010.199 kb on - strand, within CA265_RS15970 at 4010.262 kb on + strand, within CA265_RS15970 at 4010.326 kb on + strand at 4010.327 kb on - strand at 4010.469 kb on + strand at 4010.469 kb on + strand at 4010.472 kb on - strand at 4010.490 kb on + strand at 4010.490 kb on + strand at 4010.491 kb on - strand at 4010.545 kb on - strand at 4010.551 kb on + strand at 4010.620 kb on + strand at 4010.632 kb on + strand at 4010.632 kb on + strand at 4010.676 kb on + strand, within CA265_RS15975 at 4010.758 kb on + strand, within CA265_RS15975 at 4010.758 kb on + strand at 4010.758 kb on + strand, within CA265_RS15975 at 4010.777 kb on - strand, within CA265_RS15975 at 4010.808 kb on + strand, within CA265_RS15975 at 4010.815 kb on + strand, within CA265_RS15975 at 4010.815 kb on + strand, within CA265_RS15975 at 4010.837 kb on - strand, within CA265_RS15975 at 4010.853 kb on + strand, within CA265_RS15975 at 4010.895 kb on - strand, within CA265_RS15975 at 4010.900 kb on - strand, within CA265_RS15975 at 4010.900 kb on - strand, within CA265_RS15975 at 4010.946 kb on + strand, within CA265_RS15975 at 4011.069 kb on + strand, within CA265_RS15975 at 4011.075 kb on + strand, within CA265_RS15975 at 4011.088 kb on + strand, within CA265_RS15975 at 4011.109 kb on - strand, within CA265_RS15975 at 4011.109 kb on - strand, within CA265_RS15975 at 4011.269 kb on - strand, within CA265_RS15975 at 4011.269 kb on - strand, within CA265_RS15975 at 4011.469 kb on - strand at 4011.472 kb on + strand at 4011.473 kb on - strand at 4011.493 kb on - strand, within CA265_RS15980 at 4011.493 kb on - strand, within CA265_RS15980 at 4011.514 kb on + strand, within CA265_RS15980 at 4011.697 kb on + strand, within CA265_RS15980 at 4011.719 kb on - strand, within CA265_RS15980 at 4011.729 kb on - strand, within CA265_RS15980 at 4011.798 kb on + strand, within CA265_RS15980 at 4011.808 kb on + strand, within CA265_RS15980 at 4011.851 kb on - strand, within CA265_RS15980 at 4011.851 kb on - strand, within CA265_RS15980 at 4011.855 kb on + strand, within CA265_RS15980 at 4011.931 kb on - strand at 4011.945 kb on + strand at 4012.123 kb on + strand, within CA265_RS15985 at 4012.124 kb on - strand, within CA265_RS15985 at 4012.163 kb on - strand, within CA265_RS15985 at 4012.163 kb on - strand, within CA265_RS15985 at 4012.183 kb on + strand, within CA265_RS15985 at 4012.195 kb on - strand, within CA265_RS15985 at 4012.195 kb on - strand, within CA265_RS15985 at 4012.229 kb on + strand, within CA265_RS15985 at 4012.229 kb on + strand, within CA265_RS15985 at 4012.230 kb on - strand, within CA265_RS15985 at 4012.307 kb on + strand, within CA265_RS15985 at 4012.371 kb on + strand, within CA265_RS15985 at 4012.371 kb on + strand, within CA265_RS15985
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.025 mg/ml remove 4,009,960 + +0.5 4,010,008 + CA265_RS15970 0.17 -1.2 4,010,008 + CA265_RS15970 0.17 +0.7 4,010,031 + CA265_RS15970 0.23 +0.2 4,010,034 - CA265_RS15970 0.24 -0.3 4,010,037 - CA265_RS15970 0.25 +0.9 4,010,040 + CA265_RS15970 0.25 +0.4 4,010,040 + CA265_RS15970 0.25 +0.6 4,010,040 + CA265_RS15970 0.25 -1.7 4,010,044 + CA265_RS15970 0.27 +0.7 4,010,118 + CA265_RS15970 0.46 -2.2 4,010,128 + CA265_RS15970 0.49 +0.5 4,010,161 + CA265_RS15970 0.57 -0.2 4,010,161 + CA265_RS15970 0.57 +0.5 4,010,161 + CA265_RS15970 0.57 -0.2 4,010,195 - CA265_RS15970 0.66 +0.2 4,010,199 - CA265_RS15970 0.67 -1.4 4,010,262 + CA265_RS15970 0.84 +1.6 4,010,326 + +0.5 4,010,327 - -0.1 4,010,469 + +3.3 4,010,469 + +0.7 4,010,472 - +0.0 4,010,490 + +0.1 4,010,490 + -0.6 4,010,491 - -0.0 4,010,545 - -0.2 4,010,551 + +0.1 4,010,620 + +0.4 4,010,632 + +0.0 4,010,632 + +0.9 4,010,676 + CA265_RS15975 0.13 +0.4 4,010,758 + CA265_RS15975 0.23 -0.3 4,010,758 + +1.0 4,010,758 + CA265_RS15975 0.23 -0.0 4,010,777 - CA265_RS15975 0.25 -0.1 4,010,808 + CA265_RS15975 0.29 -1.2 4,010,815 + CA265_RS15975 0.30 +0.5 4,010,815 + CA265_RS15975 0.30 +1.5 4,010,837 - CA265_RS15975 0.32 +0.2 4,010,853 + CA265_RS15975 0.34 +0.2 4,010,895 - CA265_RS15975 0.39 +0.5 4,010,900 - CA265_RS15975 0.40 -0.5 4,010,900 - CA265_RS15975 0.40 +0.7 4,010,946 + CA265_RS15975 0.45 +0.4 4,011,069 + CA265_RS15975 0.60 +0.4 4,011,075 + CA265_RS15975 0.61 -0.3 4,011,088 + CA265_RS15975 0.63 +0.4 4,011,109 - CA265_RS15975 0.65 +0.5 4,011,109 - CA265_RS15975 0.65 +0.6 4,011,269 - CA265_RS15975 0.84 -0.3 4,011,269 - CA265_RS15975 0.84 -0.9 4,011,469 - +0.4 4,011,472 + -0.2 4,011,473 - +0.2 4,011,493 - CA265_RS15980 0.13 -0.7 4,011,493 - CA265_RS15980 0.13 -0.3 4,011,514 + CA265_RS15980 0.17 -0.4 4,011,697 + CA265_RS15980 0.51 -1.9 4,011,719 - CA265_RS15980 0.55 +1.3 4,011,729 - CA265_RS15980 0.57 +0.7 4,011,798 + CA265_RS15980 0.70 -0.1 4,011,808 + CA265_RS15980 0.72 -0.7 4,011,851 - CA265_RS15980 0.80 -0.0 4,011,851 - CA265_RS15980 0.80 +1.1 4,011,855 + CA265_RS15980 0.81 +0.6 4,011,931 - +0.6 4,011,945 + +0.2 4,012,123 + CA265_RS15985 0.12 -0.3 4,012,124 - CA265_RS15985 0.12 -1.7 4,012,163 - CA265_RS15985 0.15 -1.5 4,012,163 - CA265_RS15985 0.15 -0.9 4,012,183 + CA265_RS15985 0.17 -0.8 4,012,195 - CA265_RS15985 0.18 +0.1 4,012,195 - CA265_RS15985 0.18 -0.9 4,012,229 + CA265_RS15985 0.20 +1.1 4,012,229 + CA265_RS15985 0.20 -1.0 4,012,230 - CA265_RS15985 0.20 -0.2 4,012,307 + CA265_RS15985 0.26 -0.7 4,012,371 + CA265_RS15985 0.31 -0.6 4,012,371 + CA265_RS15985 0.31 -2.3
Or see this region's nucleotide sequence