Experiment: R2A with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS13715 and CA265_RS13720 are separated by 8 nucleotides CA265_RS13720 and CA265_RS13725 are separated by 10 nucleotides CA265_RS13725 and CA265_RS13730 are separated by 92 nucleotides CA265_RS13730 and CA265_RS13735 are separated by 187 nucleotides CA265_RS13735 and CA265_RS13740 are separated by 200 nucleotides
CA265_RS13715: CA265_RS13715 - glucose dehydrogenase, at 3,434,261 to 3,435,007
_RS13715
CA265_RS13720: CA265_RS13720 - 4-oxalocrotonate tautomerase, at 3,435,016 to 3,435,234
_RS13720
CA265_RS13725: CA265_RS13725 - lipid-binding protein, at 3,435,245 to 3,435,781
_RS13725
CA265_RS13730: CA265_RS13730 - Crp/Fnr family transcriptional regulator, at 3,435,874 to 3,436,452
_RS13730
CA265_RS13735: CA265_RS13735 - AsnC family transcriptional regulator, at 3,436,640 to 3,437,107
_RS13735
CA265_RS13740: CA265_RS13740 - cysteine synthase, at 3,437,308 to 3,438,354
_RS13740
Position (kb)
3435
3436
3437 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3434.889 kb on - strand, within CA265_RS13715 at 3434.889 kb on - strand, within CA265_RS13715 at 3434.915 kb on + strand, within CA265_RS13715 at 3434.965 kb on + strand at 3434.966 kb on - strand at 3434.966 kb on - strand at 3434.985 kb on - strand at 3435.035 kb on - strand at 3435.106 kb on + strand, within CA265_RS13720 at 3435.107 kb on - strand, within CA265_RS13720 at 3435.172 kb on + strand, within CA265_RS13720 at 3435.217 kb on + strand at 3435.227 kb on + strand at 3435.227 kb on + strand at 3435.250 kb on + strand at 3435.250 kb on + strand at 3435.251 kb on - strand at 3435.255 kb on - strand at 3435.293 kb on - strand at 3435.394 kb on + strand, within CA265_RS13725 at 3435.448 kb on + strand, within CA265_RS13725 at 3435.471 kb on - strand, within CA265_RS13725 at 3435.471 kb on - strand, within CA265_RS13725 at 3435.479 kb on - strand, within CA265_RS13725 at 3435.512 kb on - strand, within CA265_RS13725 at 3435.634 kb on + strand, within CA265_RS13725 at 3435.655 kb on + strand, within CA265_RS13725 at 3435.656 kb on - strand, within CA265_RS13725 at 3435.692 kb on + strand, within CA265_RS13725 at 3435.694 kb on + strand, within CA265_RS13725 at 3435.694 kb on + strand, within CA265_RS13725 at 3435.695 kb on - strand, within CA265_RS13725 at 3435.695 kb on - strand, within CA265_RS13725 at 3435.822 kb on + strand at 3435.823 kb on - strand at 3435.846 kb on - strand at 3435.854 kb on + strand at 3435.854 kb on + strand at 3435.891 kb on - strand at 3435.937 kb on + strand, within CA265_RS13730 at 3435.958 kb on - strand, within CA265_RS13730 at 3435.971 kb on + strand, within CA265_RS13730 at 3436.018 kb on - strand, within CA265_RS13730 at 3436.024 kb on + strand, within CA265_RS13730 at 3436.125 kb on + strand, within CA265_RS13730 at 3436.126 kb on - strand, within CA265_RS13730 at 3436.126 kb on - strand, within CA265_RS13730 at 3436.130 kb on - strand, within CA265_RS13730 at 3436.135 kb on + strand, within CA265_RS13730 at 3436.135 kb on + strand, within CA265_RS13730 at 3436.162 kb on + strand, within CA265_RS13730 at 3436.222 kb on - strand, within CA265_RS13730 at 3436.222 kb on - strand, within CA265_RS13730 at 3436.244 kb on + strand, within CA265_RS13730 at 3436.244 kb on + strand, within CA265_RS13730 at 3436.245 kb on - strand, within CA265_RS13730 at 3436.245 kb on - strand, within CA265_RS13730 at 3436.245 kb on - strand, within CA265_RS13730 at 3436.317 kb on + strand, within CA265_RS13730 at 3436.317 kb on + strand, within CA265_RS13730 at 3436.382 kb on + strand, within CA265_RS13730 at 3436.383 kb on - strand, within CA265_RS13730 at 3436.383 kb on - strand, within CA265_RS13730 at 3436.407 kb on + strand at 3436.462 kb on + strand at 3436.468 kb on + strand at 3436.556 kb on - strand at 3436.572 kb on + strand at 3436.598 kb on + strand at 3436.624 kb on - strand at 3436.624 kb on - strand at 3436.702 kb on + strand, within CA265_RS13735 at 3436.771 kb on + strand, within CA265_RS13735 at 3436.997 kb on - strand, within CA265_RS13735 at 3437.009 kb on - strand, within CA265_RS13735 at 3437.009 kb on - strand, within CA265_RS13735 at 3437.033 kb on - strand, within CA265_RS13735 at 3437.033 kb on - strand, within CA265_RS13735 at 3437.110 kb on - strand at 3437.134 kb on + strand at 3437.383 kb on - strand at 3437.407 kb on + strand at 3437.413 kb on + strand, within CA265_RS13740 at 3437.414 kb on - strand, within CA265_RS13740
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.025 mg/ml remove 3,434,889 - CA265_RS13715 0.84 -0.6 3,434,889 - CA265_RS13715 0.84 +0.0 3,434,915 + CA265_RS13715 0.88 -0.7 3,434,965 + -1.2 3,434,966 - -0.9 3,434,966 - +0.1 3,434,985 - +0.4 3,435,035 - +0.6 3,435,106 + CA265_RS13720 0.41 -3.0 3,435,107 - CA265_RS13720 0.42 -2.3 3,435,172 + CA265_RS13720 0.71 -0.7 3,435,217 + -0.7 3,435,227 + +0.3 3,435,227 + +2.4 3,435,250 + -0.9 3,435,250 + -0.7 3,435,251 - +0.8 3,435,255 - -0.5 3,435,293 - +0.3 3,435,394 + CA265_RS13725 0.28 +0.4 3,435,448 + CA265_RS13725 0.38 -0.7 3,435,471 - CA265_RS13725 0.42 +0.4 3,435,471 - CA265_RS13725 0.42 -0.5 3,435,479 - CA265_RS13725 0.44 +0.6 3,435,512 - CA265_RS13725 0.50 +0.9 3,435,634 + CA265_RS13725 0.72 -1.5 3,435,655 + CA265_RS13725 0.76 +0.8 3,435,656 - CA265_RS13725 0.77 -0.8 3,435,692 + CA265_RS13725 0.83 +1.1 3,435,694 + CA265_RS13725 0.84 -0.2 3,435,694 + CA265_RS13725 0.84 -0.0 3,435,695 - CA265_RS13725 0.84 -0.5 3,435,695 - CA265_RS13725 0.84 -1.0 3,435,822 + +3.7 3,435,823 - -0.2 3,435,846 - -2.0 3,435,854 + -1.3 3,435,854 + -1.9 3,435,891 - +0.4 3,435,937 + CA265_RS13730 0.11 -1.3 3,435,958 - CA265_RS13730 0.15 +0.4 3,435,971 + CA265_RS13730 0.17 -0.0 3,436,018 - CA265_RS13730 0.25 +0.2 3,436,024 + CA265_RS13730 0.26 -0.1 3,436,125 + CA265_RS13730 0.43 +0.1 3,436,126 - CA265_RS13730 0.44 +0.7 3,436,126 - CA265_RS13730 0.44 -0.4 3,436,130 - CA265_RS13730 0.44 +0.2 3,436,135 + CA265_RS13730 0.45 +0.5 3,436,135 + CA265_RS13730 0.45 -1.8 3,436,162 + CA265_RS13730 0.50 +0.8 3,436,222 - CA265_RS13730 0.60 -0.2 3,436,222 - CA265_RS13730 0.60 +0.1 3,436,244 + CA265_RS13730 0.64 +0.3 3,436,244 + CA265_RS13730 0.64 -0.1 3,436,245 - CA265_RS13730 0.64 +0.5 3,436,245 - CA265_RS13730 0.64 -1.0 3,436,245 - CA265_RS13730 0.64 +0.3 3,436,317 + CA265_RS13730 0.77 +0.4 3,436,317 + CA265_RS13730 0.77 +1.1 3,436,382 + CA265_RS13730 0.88 -0.3 3,436,383 - CA265_RS13730 0.88 +0.4 3,436,383 - CA265_RS13730 0.88 +0.8 3,436,407 + +0.2 3,436,462 + -0.0 3,436,468 + +0.0 3,436,556 - -0.2 3,436,572 + -0.2 3,436,598 + -0.6 3,436,624 - -0.3 3,436,624 - -2.0 3,436,702 + CA265_RS13735 0.13 +1.5 3,436,771 + CA265_RS13735 0.28 +3.1 3,436,997 - CA265_RS13735 0.76 +0.0 3,437,009 - CA265_RS13735 0.79 +0.6 3,437,009 - CA265_RS13735 0.79 +0.5 3,437,033 - CA265_RS13735 0.84 -1.8 3,437,033 - CA265_RS13735 0.84 -0.3 3,437,110 - -0.0 3,437,134 + +1.0 3,437,383 - +0.0 3,437,407 + +0.2 3,437,413 + CA265_RS13740 0.10 -0.7 3,437,414 - CA265_RS13740 0.10 +0.5
Or see this region's nucleotide sequence