Experiment: R2A with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11350 and CA265_RS11355 are separated by 120 nucleotides CA265_RS11355 and CA265_RS11360 are separated by 18 nucleotides
CA265_RS11350: CA265_RS11350 - hypothetical protein, at 2,816,346 to 2,817,959
_RS11350
CA265_RS11355: CA265_RS11355 - hypothetical protein, at 2,818,080 to 2,819,402
_RS11355
CA265_RS11360: CA265_RS11360 - nucleotide pyrophosphatase, at 2,819,421 to 2,820,323
_RS11360
Position (kb)
2818
2819
2820 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2817.131 kb on + strand, within CA265_RS11350 at 2817.227 kb on + strand, within CA265_RS11350 at 2817.227 kb on + strand, within CA265_RS11350 at 2817.271 kb on - strand, within CA265_RS11350 at 2817.326 kb on + strand, within CA265_RS11350 at 2817.326 kb on + strand, within CA265_RS11350 at 2817.336 kb on - strand, within CA265_RS11350 at 2817.359 kb on + strand, within CA265_RS11350 at 2817.359 kb on + strand, within CA265_RS11350 at 2817.360 kb on - strand, within CA265_RS11350 at 2817.360 kb on - strand, within CA265_RS11350 at 2817.383 kb on + strand, within CA265_RS11350 at 2817.384 kb on - strand, within CA265_RS11350 at 2817.396 kb on - strand, within CA265_RS11350 at 2817.398 kb on + strand, within CA265_RS11350 at 2817.479 kb on + strand, within CA265_RS11350 at 2817.563 kb on + strand, within CA265_RS11350 at 2817.580 kb on + strand, within CA265_RS11350 at 2817.580 kb on + strand, within CA265_RS11350 at 2817.678 kb on + strand, within CA265_RS11350 at 2817.698 kb on - strand, within CA265_RS11350 at 2817.699 kb on + strand, within CA265_RS11350 at 2817.700 kb on - strand, within CA265_RS11350 at 2817.725 kb on + strand, within CA265_RS11350 at 2817.726 kb on - strand, within CA265_RS11350 at 2817.735 kb on - strand, within CA265_RS11350 at 2817.777 kb on + strand, within CA265_RS11350 at 2817.799 kb on - strand at 2817.843 kb on - strand at 2817.933 kb on + strand at 2817.953 kb on + strand at 2818.033 kb on - strand at 2818.154 kb on + strand at 2818.161 kb on + strand at 2818.161 kb on + strand at 2818.161 kb on + strand at 2818.162 kb on - strand at 2818.196 kb on - strand at 2818.248 kb on + strand, within CA265_RS11355 at 2818.248 kb on + strand, within CA265_RS11355 at 2818.248 kb on + strand, within CA265_RS11355 at 2818.325 kb on + strand, within CA265_RS11355 at 2818.326 kb on - strand, within CA265_RS11355 at 2818.380 kb on - strand, within CA265_RS11355 at 2818.402 kb on - strand, within CA265_RS11355 at 2818.412 kb on - strand, within CA265_RS11355 at 2818.499 kb on - strand, within CA265_RS11355 at 2818.541 kb on + strand, within CA265_RS11355 at 2818.574 kb on + strand, within CA265_RS11355 at 2818.691 kb on - strand, within CA265_RS11355 at 2818.696 kb on - strand, within CA265_RS11355 at 2818.767 kb on + strand, within CA265_RS11355 at 2818.956 kb on + strand, within CA265_RS11355 at 2818.995 kb on - strand, within CA265_RS11355 at 2819.033 kb on - strand, within CA265_RS11355 at 2819.083 kb on + strand, within CA265_RS11355 at 2819.084 kb on - strand, within CA265_RS11355 at 2819.084 kb on - strand, within CA265_RS11355 at 2819.193 kb on + strand, within CA265_RS11355 at 2819.201 kb on + strand, within CA265_RS11355 at 2819.262 kb on - strand, within CA265_RS11355 at 2819.362 kb on - strand at 2819.371 kb on - strand at 2819.377 kb on + strand at 2819.497 kb on - strand at 2819.499 kb on + strand at 2819.523 kb on - strand, within CA265_RS11360 at 2819.532 kb on - strand, within CA265_RS11360 at 2819.615 kb on + strand, within CA265_RS11360 at 2819.616 kb on - strand, within CA265_RS11360 at 2819.616 kb on - strand, within CA265_RS11360 at 2819.619 kb on + strand, within CA265_RS11360 at 2819.691 kb on + strand, within CA265_RS11360 at 2819.691 kb on + strand, within CA265_RS11360 at 2819.692 kb on - strand, within CA265_RS11360 at 2819.700 kb on + strand, within CA265_RS11360 at 2819.721 kb on + strand, within CA265_RS11360 at 2819.782 kb on - strand, within CA265_RS11360 at 2819.786 kb on + strand, within CA265_RS11360 at 2819.786 kb on + strand, within CA265_RS11360 at 2819.788 kb on + strand, within CA265_RS11360 at 2819.790 kb on + strand, within CA265_RS11360 at 2819.791 kb on - strand, within CA265_RS11360 at 2819.798 kb on + strand, within CA265_RS11360 at 2819.799 kb on - strand, within CA265_RS11360 at 2819.858 kb on + strand, within CA265_RS11360 at 2819.859 kb on - strand, within CA265_RS11360 at 2819.883 kb on + strand, within CA265_RS11360 at 2819.936 kb on - strand, within CA265_RS11360 at 2819.942 kb on - strand at 2819.961 kb on + strand, within CA265_RS11360 at 2819.961 kb on + strand, within CA265_RS11360 at 2819.976 kb on + strand, within CA265_RS11360 at 2819.977 kb on - strand, within CA265_RS11360 at 2819.981 kb on + strand, within CA265_RS11360 at 2819.982 kb on - strand, within CA265_RS11360 at 2820.027 kb on + strand, within CA265_RS11360 at 2820.027 kb on + strand, within CA265_RS11360 at 2820.028 kb on - strand, within CA265_RS11360 at 2820.028 kb on - strand, within CA265_RS11360 at 2820.119 kb on + strand, within CA265_RS11360 at 2820.157 kb on - strand, within CA265_RS11360 at 2820.269 kb on + strand at 2820.283 kb on - strand at 2820.286 kb on - strand at 2820.342 kb on - strand at 2820.342 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.025 mg/ml remove 2,817,131 + CA265_RS11350 0.49 +0.7 2,817,227 + CA265_RS11350 0.55 +0.8 2,817,227 + CA265_RS11350 0.55 -0.3 2,817,271 - CA265_RS11350 0.57 -0.8 2,817,326 + CA265_RS11350 0.61 -1.0 2,817,326 + CA265_RS11350 0.61 -0.1 2,817,336 - CA265_RS11350 0.61 -0.8 2,817,359 + CA265_RS11350 0.63 -0.2 2,817,359 + CA265_RS11350 0.63 +0.2 2,817,360 - CA265_RS11350 0.63 +0.3 2,817,360 - CA265_RS11350 0.63 +1.0 2,817,383 + CA265_RS11350 0.64 -0.8 2,817,384 - CA265_RS11350 0.64 +0.3 2,817,396 - CA265_RS11350 0.65 +0.1 2,817,398 + CA265_RS11350 0.65 +0.3 2,817,479 + CA265_RS11350 0.70 -0.2 2,817,563 + CA265_RS11350 0.75 +0.2 2,817,580 + CA265_RS11350 0.76 -0.2 2,817,580 + CA265_RS11350 0.76 -0.6 2,817,678 + CA265_RS11350 0.83 +0.3 2,817,698 - CA265_RS11350 0.84 -0.9 2,817,699 + CA265_RS11350 0.84 -1.5 2,817,700 - CA265_RS11350 0.84 -0.2 2,817,725 + CA265_RS11350 0.85 +2.0 2,817,726 - CA265_RS11350 0.86 -1.4 2,817,735 - CA265_RS11350 0.86 +1.8 2,817,777 + CA265_RS11350 0.89 -2.3 2,817,799 - +0.0 2,817,843 - -0.5 2,817,933 + -1.2 2,817,953 + -1.4 2,818,033 - +1.3 2,818,154 + +1.7 2,818,161 + +0.5 2,818,161 + +0.3 2,818,161 + -0.2 2,818,162 - -1.6 2,818,196 - -1.6 2,818,248 + CA265_RS11355 0.13 -0.5 2,818,248 + CA265_RS11355 0.13 -0.7 2,818,248 + CA265_RS11355 0.13 -0.0 2,818,325 + CA265_RS11355 0.19 +0.6 2,818,326 - CA265_RS11355 0.19 -0.6 2,818,380 - CA265_RS11355 0.23 -2.3 2,818,402 - CA265_RS11355 0.24 -1.9 2,818,412 - CA265_RS11355 0.25 +0.3 2,818,499 - CA265_RS11355 0.32 +0.6 2,818,541 + CA265_RS11355 0.35 +0.2 2,818,574 + CA265_RS11355 0.37 -0.7 2,818,691 - CA265_RS11355 0.46 +0.5 2,818,696 - CA265_RS11355 0.47 -0.5 2,818,767 + CA265_RS11355 0.52 +0.5 2,818,956 + CA265_RS11355 0.66 -0.5 2,818,995 - CA265_RS11355 0.69 -0.2 2,819,033 - CA265_RS11355 0.72 -0.0 2,819,083 + CA265_RS11355 0.76 -0.3 2,819,084 - CA265_RS11355 0.76 -1.0 2,819,084 - CA265_RS11355 0.76 -0.7 2,819,193 + CA265_RS11355 0.84 +0.5 2,819,201 + CA265_RS11355 0.85 -1.2 2,819,262 - CA265_RS11355 0.89 -1.5 2,819,362 - -0.8 2,819,371 - +0.7 2,819,377 + -1.1 2,819,497 - +0.1 2,819,499 + +0.5 2,819,523 - CA265_RS11360 0.11 -0.5 2,819,532 - CA265_RS11360 0.12 +0.4 2,819,615 + CA265_RS11360 0.21 -1.9 2,819,616 - CA265_RS11360 0.22 +0.1 2,819,616 - CA265_RS11360 0.22 -0.9 2,819,619 + CA265_RS11360 0.22 -0.1 2,819,691 + CA265_RS11360 0.30 -0.6 2,819,691 + CA265_RS11360 0.30 -1.5 2,819,692 - CA265_RS11360 0.30 -0.1 2,819,700 + CA265_RS11360 0.31 -0.3 2,819,721 + CA265_RS11360 0.33 -1.3 2,819,782 - CA265_RS11360 0.40 +0.5 2,819,786 + CA265_RS11360 0.40 +0.0 2,819,786 + CA265_RS11360 0.40 +0.5 2,819,788 + CA265_RS11360 0.41 -0.7 2,819,790 + CA265_RS11360 0.41 -0.8 2,819,791 - CA265_RS11360 0.41 -1.2 2,819,798 + CA265_RS11360 0.42 -0.0 2,819,799 - CA265_RS11360 0.42 -0.1 2,819,858 + CA265_RS11360 0.48 -0.5 2,819,859 - CA265_RS11360 0.49 -0.0 2,819,883 + CA265_RS11360 0.51 -1.9 2,819,936 - CA265_RS11360 0.57 -0.3 2,819,942 - +1.4 2,819,961 + CA265_RS11360 0.60 -2.9 2,819,961 + CA265_RS11360 0.60 +0.2 2,819,976 + CA265_RS11360 0.61 -0.6 2,819,977 - CA265_RS11360 0.62 +0.0 2,819,981 + CA265_RS11360 0.62 +0.1 2,819,982 - CA265_RS11360 0.62 -0.1 2,820,027 + CA265_RS11360 0.67 -0.1 2,820,027 + CA265_RS11360 0.67 -0.0 2,820,028 - CA265_RS11360 0.67 -1.0 2,820,028 - CA265_RS11360 0.67 -0.5 2,820,119 + CA265_RS11360 0.77 +0.4 2,820,157 - CA265_RS11360 0.82 -1.0 2,820,269 + +0.6 2,820,283 - -0.5 2,820,286 - -0.5 2,820,342 - +0.5 2,820,342 - -0.8
Or see this region's nucleotide sequence