Experiment: R2A with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11240 and CA265_RS11245 are separated by 64 nucleotides CA265_RS11245 and CA265_RS11250 are separated by 207 nucleotides
CA265_RS11240: CA265_RS11240 - hypothetical protein, at 2,781,299 to 2,781,997
_RS11240
CA265_RS11245: CA265_RS11245 - hypothetical protein, at 2,782,062 to 2,782,913
_RS11245
CA265_RS11250: CA265_RS11250 - hypothetical protein, at 2,783,121 to 2,784,191
_RS11250
Position (kb)
2782
2783 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2781.063 kb on + strand at 2781.110 kb on + strand at 2781.111 kb on - strand at 2781.133 kb on - strand at 2781.193 kb on + strand at 2781.242 kb on - strand at 2781.312 kb on + strand at 2781.394 kb on + strand, within CA265_RS11240 at 2781.395 kb on - strand, within CA265_RS11240 at 2781.408 kb on - strand, within CA265_RS11240 at 2781.503 kb on - strand, within CA265_RS11240 at 2781.503 kb on - strand, within CA265_RS11240 at 2781.505 kb on - strand, within CA265_RS11240 at 2781.588 kb on + strand, within CA265_RS11240 at 2781.590 kb on + strand, within CA265_RS11240 at 2781.590 kb on + strand, within CA265_RS11240 at 2781.607 kb on - strand, within CA265_RS11240 at 2781.636 kb on - strand, within CA265_RS11240 at 2781.650 kb on - strand, within CA265_RS11240 at 2781.729 kb on + strand, within CA265_RS11240 at 2781.820 kb on - strand, within CA265_RS11240 at 2781.833 kb on - strand, within CA265_RS11240 at 2781.833 kb on - strand, within CA265_RS11240 at 2781.833 kb on - strand, within CA265_RS11240 at 2781.833 kb on - strand, within CA265_RS11240 at 2781.852 kb on - strand, within CA265_RS11240 at 2781.879 kb on - strand, within CA265_RS11240 at 2781.938 kb on - strand at 2782.014 kb on - strand at 2782.019 kb on + strand at 2782.041 kb on - strand at 2782.064 kb on - strand at 2782.076 kb on - strand at 2782.116 kb on + strand at 2782.167 kb on - strand, within CA265_RS11245 at 2782.192 kb on + strand, within CA265_RS11245 at 2782.192 kb on + strand, within CA265_RS11245 at 2782.257 kb on + strand, within CA265_RS11245 at 2782.258 kb on - strand, within CA265_RS11245 at 2782.345 kb on + strand, within CA265_RS11245 at 2782.349 kb on + strand, within CA265_RS11245 at 2782.388 kb on - strand, within CA265_RS11245 at 2782.454 kb on + strand, within CA265_RS11245 at 2782.455 kb on - strand, within CA265_RS11245 at 2782.458 kb on + strand, within CA265_RS11245 at 2782.458 kb on + strand, within CA265_RS11245 at 2782.578 kb on - strand, within CA265_RS11245 at 2782.598 kb on + strand, within CA265_RS11245 at 2782.603 kb on + strand, within CA265_RS11245 at 2782.603 kb on + strand, within CA265_RS11245 at 2782.603 kb on + strand, within CA265_RS11245 at 2782.604 kb on - strand, within CA265_RS11245 at 2782.604 kb on - strand at 2782.604 kb on - strand, within CA265_RS11245 at 2782.607 kb on - strand, within CA265_RS11245 at 2782.627 kb on + strand, within CA265_RS11245 at 2782.628 kb on - strand, within CA265_RS11245 at 2782.628 kb on - strand, within CA265_RS11245 at 2782.729 kb on + strand, within CA265_RS11245 at 2782.881 kb on - strand at 2782.884 kb on - strand at 2782.916 kb on - strand at 2782.916 kb on - strand at 2782.916 kb on - strand at 2783.001 kb on - strand at 2783.024 kb on - strand at 2783.134 kb on - strand at 2783.162 kb on + strand at 2783.185 kb on + strand at 2783.199 kb on - strand at 2783.325 kb on - strand, within CA265_RS11250 at 2783.329 kb on + strand, within CA265_RS11250 at 2783.334 kb on + strand, within CA265_RS11250 at 2783.443 kb on - strand, within CA265_RS11250 at 2783.515 kb on + strand, within CA265_RS11250 at 2783.517 kb on + strand, within CA265_RS11250 at 2783.518 kb on - strand, within CA265_RS11250 at 2783.533 kb on + strand, within CA265_RS11250 at 2783.629 kb on + strand, within CA265_RS11250 at 2783.629 kb on + strand, within CA265_RS11250 at 2783.637 kb on - strand, within CA265_RS11250 at 2783.777 kb on + strand, within CA265_RS11250 at 2783.806 kb on + strand, within CA265_RS11250 at 2783.807 kb on - strand, within CA265_RS11250 at 2783.830 kb on + strand, within CA265_RS11250 at 2783.831 kb on - strand, within CA265_RS11250 at 2783.875 kb on + strand, within CA265_RS11250
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.025 mg/ml remove 2,781,063 + -0.1 2,781,110 + +0.3 2,781,111 - +0.2 2,781,133 - -0.1 2,781,193 + +0.0 2,781,242 - +0.2 2,781,312 + +1.2 2,781,394 + CA265_RS11240 0.14 +0.3 2,781,395 - CA265_RS11240 0.14 -1.3 2,781,408 - CA265_RS11240 0.16 -0.5 2,781,503 - CA265_RS11240 0.29 -0.3 2,781,503 - CA265_RS11240 0.29 +0.1 2,781,505 - CA265_RS11240 0.29 +0.6 2,781,588 + CA265_RS11240 0.41 +0.1 2,781,590 + CA265_RS11240 0.42 -0.3 2,781,590 + CA265_RS11240 0.42 -0.2 2,781,607 - CA265_RS11240 0.44 -0.7 2,781,636 - CA265_RS11240 0.48 -0.3 2,781,650 - CA265_RS11240 0.50 +0.2 2,781,729 + CA265_RS11240 0.62 +1.3 2,781,820 - CA265_RS11240 0.75 +0.0 2,781,833 - CA265_RS11240 0.76 -1.2 2,781,833 - CA265_RS11240 0.76 -0.6 2,781,833 - CA265_RS11240 0.76 -0.6 2,781,833 - CA265_RS11240 0.76 -2.3 2,781,852 - CA265_RS11240 0.79 -0.1 2,781,879 - CA265_RS11240 0.83 -0.3 2,781,938 - -0.3 2,782,014 - -0.3 2,782,019 + -0.1 2,782,041 - -0.4 2,782,064 - -0.5 2,782,076 - -2.0 2,782,116 + +1.0 2,782,167 - CA265_RS11245 0.12 +0.2 2,782,192 + CA265_RS11245 0.15 -0.2 2,782,192 + CA265_RS11245 0.15 -0.4 2,782,257 + CA265_RS11245 0.23 -0.3 2,782,258 - CA265_RS11245 0.23 -0.3 2,782,345 + CA265_RS11245 0.33 +0.6 2,782,349 + CA265_RS11245 0.34 +0.3 2,782,388 - CA265_RS11245 0.38 +0.1 2,782,454 + CA265_RS11245 0.46 -0.1 2,782,455 - CA265_RS11245 0.46 -0.5 2,782,458 + CA265_RS11245 0.46 -0.1 2,782,458 + CA265_RS11245 0.46 +0.2 2,782,578 - CA265_RS11245 0.61 +0.8 2,782,598 + CA265_RS11245 0.63 -1.0 2,782,603 + CA265_RS11245 0.63 -1.3 2,782,603 + CA265_RS11245 0.63 -1.2 2,782,603 + CA265_RS11245 0.63 +0.6 2,782,604 - CA265_RS11245 0.64 -0.3 2,782,604 - +1.4 2,782,604 - CA265_RS11245 0.64 +1.2 2,782,607 - CA265_RS11245 0.64 -0.7 2,782,627 + CA265_RS11245 0.66 +0.0 2,782,628 - CA265_RS11245 0.66 -1.2 2,782,628 - CA265_RS11245 0.66 -0.6 2,782,729 + CA265_RS11245 0.78 +0.0 2,782,881 - +2.0 2,782,884 - +0.0 2,782,916 - -0.6 2,782,916 - +0.2 2,782,916 - -0.8 2,783,001 - -1.7 2,783,024 - -0.5 2,783,134 - -1.6 2,783,162 + +0.5 2,783,185 + -2.5 2,783,199 - +1.0 2,783,325 - CA265_RS11250 0.19 -0.9 2,783,329 + CA265_RS11250 0.19 -0.2 2,783,334 + CA265_RS11250 0.20 +0.1 2,783,443 - CA265_RS11250 0.30 +0.2 2,783,515 + CA265_RS11250 0.37 -2.5 2,783,517 + CA265_RS11250 0.37 +0.3 2,783,518 - CA265_RS11250 0.37 -2.0 2,783,533 + CA265_RS11250 0.38 -0.7 2,783,629 + CA265_RS11250 0.47 -0.6 2,783,629 + CA265_RS11250 0.47 -0.9 2,783,637 - CA265_RS11250 0.48 +0.2 2,783,777 + CA265_RS11250 0.61 -0.5 2,783,806 + CA265_RS11250 0.64 -0.6 2,783,807 - CA265_RS11250 0.64 -0.8 2,783,830 + CA265_RS11250 0.66 -0.8 2,783,831 - CA265_RS11250 0.66 -0.5 2,783,875 + CA265_RS11250 0.70 -1.0
Or see this region's nucleotide sequence