Experiment: R2A with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03070 and CA265_RS03075 are separated by 9 nucleotides CA265_RS03075 and CA265_RS03080 are separated by 172 nucleotides CA265_RS03080 and CA265_RS03085 are separated by 150 nucleotides
CA265_RS03070: CA265_RS03070 - sec-independent protein translocase TatC, at 761,144 to 761,539
_RS03070
CA265_RS03075: CA265_RS03075 - hypothetical protein, at 761,549 to 761,974
_RS03075
CA265_RS03080: CA265_RS03080 - malate dehydrogenase, at 762,147 to 763,085
_RS03080
CA265_RS03085: CA265_RS03085 - hypothetical protein, at 763,236 to 763,688
_RS03085
Position (kb) 762
763
764 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 761.236 kb on + strand, within CA265_RS03070 at 761.236 kb on + strand, within CA265_RS03070 at 761.324 kb on + strand, within CA265_RS03070 at 761.357 kb on - strand, within CA265_RS03070 at 761.359 kb on - strand, within CA265_RS03070 at 761.427 kb on - strand, within CA265_RS03070 at 761.447 kb on + strand, within CA265_RS03070 at 761.458 kb on + strand, within CA265_RS03070 at 761.468 kb on - strand, within CA265_RS03070 at 761.492 kb on - strand, within CA265_RS03070 at 761.492 kb on - strand, within CA265_RS03070 at 761.509 kb on + strand at 761.510 kb on - strand at 761.547 kb on + strand at 761.547 kb on + strand at 761.548 kb on - strand at 761.608 kb on + strand, within CA265_RS03075 at 761.609 kb on + strand, within CA265_RS03075 at 761.610 kb on - strand, within CA265_RS03075 at 761.610 kb on - strand, within CA265_RS03075 at 761.610 kb on - strand, within CA265_RS03075 at 761.626 kb on + strand, within CA265_RS03075 at 761.626 kb on + strand, within CA265_RS03075 at 761.631 kb on - strand, within CA265_RS03075 at 761.632 kb on + strand, within CA265_RS03075 at 761.632 kb on + strand, within CA265_RS03075 at 761.632 kb on + strand, within CA265_RS03075 at 761.633 kb on - strand, within CA265_RS03075 at 761.785 kb on + strand, within CA265_RS03075 at 761.805 kb on - strand, within CA265_RS03075 at 761.839 kb on + strand, within CA265_RS03075 at 761.851 kb on + strand, within CA265_RS03075 at 761.866 kb on + strand, within CA265_RS03075 at 761.866 kb on + strand, within CA265_RS03075 at 761.979 kb on + strand at 761.979 kb on + strand at 762.023 kb on - strand at 762.048 kb on + strand at 762.057 kb on - strand at 762.067 kb on + strand at 762.067 kb on + strand at 762.068 kb on - strand at 762.068 kb on - strand at 762.068 kb on - strand at 762.093 kb on - strand at 762.137 kb on + strand at 762.137 kb on + strand at 762.182 kb on + strand at 762.275 kb on + strand, within CA265_RS03080 at 762.286 kb on - strand, within CA265_RS03080 at 762.291 kb on + strand, within CA265_RS03080 at 762.300 kb on - strand, within CA265_RS03080 at 762.426 kb on + strand, within CA265_RS03080 at 762.458 kb on + strand, within CA265_RS03080 at 762.483 kb on - strand, within CA265_RS03080 at 762.501 kb on - strand, within CA265_RS03080 at 762.520 kb on + strand, within CA265_RS03080 at 762.555 kb on - strand, within CA265_RS03080 at 762.607 kb on - strand, within CA265_RS03080 at 762.623 kb on - strand, within CA265_RS03080 at 762.706 kb on + strand, within CA265_RS03080 at 762.707 kb on - strand, within CA265_RS03080 at 762.803 kb on + strand, within CA265_RS03080 at 762.806 kb on + strand, within CA265_RS03080 at 762.858 kb on - strand, within CA265_RS03080 at 762.892 kb on + strand, within CA265_RS03080 at 762.993 kb on + strand at 763.115 kb on + strand at 763.145 kb on + strand at 763.196 kb on - strand at 763.198 kb on + strand at 763.269 kb on - strand at 763.284 kb on - strand, within CA265_RS03085 at 763.290 kb on + strand, within CA265_RS03085 at 763.291 kb on - strand, within CA265_RS03085 at 763.341 kb on - strand, within CA265_RS03085 at 763.385 kb on + strand, within CA265_RS03085 at 763.388 kb on + strand, within CA265_RS03085 at 763.389 kb on - strand, within CA265_RS03085 at 763.389 kb on - strand, within CA265_RS03085 at 763.497 kb on - strand, within CA265_RS03085 at 763.535 kb on + strand, within CA265_RS03085 at 763.536 kb on - strand, within CA265_RS03085 at 763.540 kb on - strand, within CA265_RS03085 at 763.550 kb on - strand, within CA265_RS03085 at 763.569 kb on + strand, within CA265_RS03085 at 763.718 kb on + strand at 763.736 kb on - strand at 763.784 kb on + strand at 763.785 kb on - strand at 763.831 kb on + strand at 763.845 kb on - strand at 763.851 kb on - strand at 763.855 kb on + strand at 763.855 kb on + strand at 763.856 kb on - strand at 763.924 kb on - strand at 763.936 kb on + strand at 763.943 kb on + strand at 763.999 kb on - strand at 764.021 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.025 mg/ml remove 761,236 + CA265_RS03070 0.23 +0.0 761,236 + CA265_RS03070 0.23 -1.1 761,324 + CA265_RS03070 0.45 -0.2 761,357 - CA265_RS03070 0.54 +0.1 761,359 - CA265_RS03070 0.54 -0.5 761,427 - CA265_RS03070 0.71 +0.5 761,447 + CA265_RS03070 0.77 +0.8 761,458 + CA265_RS03070 0.79 +0.1 761,468 - CA265_RS03070 0.82 +2.5 761,492 - CA265_RS03070 0.88 +0.3 761,492 - CA265_RS03070 0.88 -0.1 761,509 + +0.2 761,510 - -0.7 761,547 + -1.0 761,547 + +0.1 761,548 - +0.1 761,608 + CA265_RS03075 0.14 +0.2 761,609 + CA265_RS03075 0.14 -0.5 761,610 - CA265_RS03075 0.14 -0.9 761,610 - CA265_RS03075 0.14 +1.8 761,610 - CA265_RS03075 0.14 -1.4 761,626 + CA265_RS03075 0.18 +0.0 761,626 + CA265_RS03075 0.18 -0.2 761,631 - CA265_RS03075 0.19 +0.6 761,632 + CA265_RS03075 0.19 -1.0 761,632 + CA265_RS03075 0.19 +0.3 761,632 + CA265_RS03075 0.19 -0.7 761,633 - CA265_RS03075 0.20 +0.0 761,785 + CA265_RS03075 0.55 +0.0 761,805 - CA265_RS03075 0.60 +1.0 761,839 + CA265_RS03075 0.68 -1.1 761,851 + CA265_RS03075 0.71 -0.6 761,866 + CA265_RS03075 0.74 -0.1 761,866 + CA265_RS03075 0.74 -0.8 761,979 + -1.4 761,979 + -0.5 762,023 - -0.8 762,048 + -0.4 762,057 - +1.1 762,067 + -0.6 762,067 + -1.3 762,068 - +0.0 762,068 - -0.1 762,068 - -0.2 762,093 - -1.0 762,137 + -1.3 762,137 + -0.0 762,182 + -0.2 762,275 + CA265_RS03080 0.14 -1.3 762,286 - CA265_RS03080 0.15 -0.2 762,291 + CA265_RS03080 0.15 +0.3 762,300 - CA265_RS03080 0.16 +0.0 762,426 + CA265_RS03080 0.30 +0.2 762,458 + CA265_RS03080 0.33 -1.2 762,483 - CA265_RS03080 0.36 -0.6 762,501 - CA265_RS03080 0.38 -0.1 762,520 + CA265_RS03080 0.40 -0.5 762,555 - CA265_RS03080 0.43 +0.2 762,607 - CA265_RS03080 0.49 -1.4 762,623 - CA265_RS03080 0.51 -0.7 762,706 + CA265_RS03080 0.60 -0.3 762,707 - CA265_RS03080 0.60 -0.5 762,803 + CA265_RS03080 0.70 -0.1 762,806 + CA265_RS03080 0.70 -1.5 762,858 - CA265_RS03080 0.76 -0.2 762,892 + CA265_RS03080 0.79 -0.8 762,993 + -0.3 763,115 + -0.3 763,145 + +0.7 763,196 - -0.2 763,198 + -1.9 763,269 - -1.1 763,284 - CA265_RS03085 0.11 +1.9 763,290 + CA265_RS03085 0.12 +0.6 763,291 - CA265_RS03085 0.12 +0.1 763,341 - CA265_RS03085 0.23 +0.9 763,385 + CA265_RS03085 0.33 -0.8 763,388 + CA265_RS03085 0.34 +1.7 763,389 - CA265_RS03085 0.34 +2.6 763,389 - CA265_RS03085 0.34 +1.3 763,497 - CA265_RS03085 0.58 +0.9 763,535 + CA265_RS03085 0.66 +2.9 763,536 - CA265_RS03085 0.66 +1.0 763,540 - CA265_RS03085 0.67 -1.0 763,550 - CA265_RS03085 0.69 -0.3 763,569 + CA265_RS03085 0.74 +0.5 763,718 + +1.2 763,736 - -2.0 763,784 + +0.2 763,785 - -0.8 763,831 + +0.3 763,845 - -0.7 763,851 - -0.9 763,855 + +0.4 763,855 + -0.1 763,856 - -0.5 763,924 - +0.3 763,936 + -0.3 763,943 + -0.3 763,999 - +1.4 764,021 - -0.1
Or see this region's nucleotide sequence