Experiment: R2A with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03045 and CA265_RS03050 are separated by 24 nucleotides CA265_RS03050 and CA265_RS03055 are separated by 201 nucleotides CA265_RS03055 and CA265_RS03060 overlap by 1 nucleotides CA265_RS03060 and CA265_RS03065 are separated by 238 nucleotides
CA265_RS03045: CA265_RS03045 - hypothetical protein, at 756,625 to 757,551
_RS03045
CA265_RS03050: CA265_RS03050 - hypothetical protein, at 757,576 to 758,073
_RS03050
CA265_RS03055: CA265_RS03055 - two-component sensor histidine kinase, at 758,275 to 759,627
_RS03055
CA265_RS03060: CA265_RS03060 - DNA-binding response regulator, at 759,627 to 760,304
_RS03060
CA265_RS03065: CA265_RS03065 - hypothetical protein, at 760,543 to 761,079
_RS03065
Position (kb)
758
759
760 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 757.288 kb on + strand, within CA265_RS03045 at 757.344 kb on + strand, within CA265_RS03045 at 757.344 kb on + strand, within CA265_RS03045 at 757.345 kb on - strand, within CA265_RS03045 at 757.360 kb on - strand, within CA265_RS03045 at 757.451 kb on + strand, within CA265_RS03045 at 757.453 kb on + strand, within CA265_RS03045 at 757.454 kb on - strand, within CA265_RS03045 at 757.457 kb on - strand, within CA265_RS03045 at 757.464 kb on + strand at 757.465 kb on - strand at 757.468 kb on + strand at 757.518 kb on + strand at 757.519 kb on - strand at 757.519 kb on - strand at 757.521 kb on - strand at 757.521 kb on - strand at 757.707 kb on + strand, within CA265_RS03050 at 757.712 kb on + strand, within CA265_RS03050 at 757.713 kb on - strand, within CA265_RS03050 at 757.713 kb on - strand, within CA265_RS03050 at 757.716 kb on + strand, within CA265_RS03050 at 757.748 kb on + strand, within CA265_RS03050 at 757.803 kb on + strand, within CA265_RS03050 at 757.817 kb on + strand, within CA265_RS03050 at 757.931 kb on - strand, within CA265_RS03050 at 757.935 kb on + strand, within CA265_RS03050 at 757.936 kb on - strand, within CA265_RS03050 at 757.959 kb on + strand, within CA265_RS03050 at 758.060 kb on - strand at 758.079 kb on + strand at 758.080 kb on - strand at 758.200 kb on - strand at 758.200 kb on - strand at 758.207 kb on + strand at 758.230 kb on + strand at 758.231 kb on - strand at 758.277 kb on - strand at 758.338 kb on + strand at 758.343 kb on + strand at 758.353 kb on + strand at 758.365 kb on - strand at 758.380 kb on + strand at 758.380 kb on + strand at 758.394 kb on - strand at 758.427 kb on - strand, within CA265_RS03055 at 758.427 kb on - strand, within CA265_RS03055 at 758.440 kb on + strand, within CA265_RS03055 at 758.441 kb on - strand, within CA265_RS03055 at 758.442 kb on + strand, within CA265_RS03055 at 758.443 kb on - strand, within CA265_RS03055 at 758.452 kb on - strand, within CA265_RS03055 at 758.485 kb on + strand, within CA265_RS03055 at 758.503 kb on - strand, within CA265_RS03055 at 758.520 kb on + strand, within CA265_RS03055 at 758.523 kb on + strand, within CA265_RS03055 at 758.524 kb on - strand, within CA265_RS03055 at 758.554 kb on - strand, within CA265_RS03055 at 758.554 kb on - strand, within CA265_RS03055 at 758.571 kb on + strand, within CA265_RS03055 at 758.620 kb on + strand, within CA265_RS03055 at 758.621 kb on - strand, within CA265_RS03055 at 758.646 kb on + strand, within CA265_RS03055 at 758.647 kb on - strand, within CA265_RS03055 at 758.697 kb on + strand, within CA265_RS03055 at 758.725 kb on + strand, within CA265_RS03055 at 758.725 kb on + strand, within CA265_RS03055 at 758.793 kb on + strand, within CA265_RS03055 at 758.794 kb on - strand, within CA265_RS03055 at 758.800 kb on + strand, within CA265_RS03055 at 758.800 kb on + strand, within CA265_RS03055 at 758.896 kb on - strand, within CA265_RS03055 at 758.914 kb on + strand, within CA265_RS03055 at 758.914 kb on + strand, within CA265_RS03055 at 758.915 kb on - strand, within CA265_RS03055 at 758.937 kb on + strand, within CA265_RS03055 at 759.037 kb on + strand, within CA265_RS03055 at 759.038 kb on - strand, within CA265_RS03055 at 759.072 kb on + strand, within CA265_RS03055 at 759.085 kb on + strand, within CA265_RS03055 at 759.103 kb on + strand, within CA265_RS03055 at 759.111 kb on - strand, within CA265_RS03055 at 759.115 kb on - strand, within CA265_RS03055 at 759.143 kb on - strand, within CA265_RS03055 at 759.238 kb on - strand, within CA265_RS03055 at 759.304 kb on + strand, within CA265_RS03055 at 759.382 kb on + strand, within CA265_RS03055 at 759.383 kb on - strand, within CA265_RS03055 at 759.383 kb on - strand, within CA265_RS03055 at 759.390 kb on - strand, within CA265_RS03055 at 759.440 kb on + strand, within CA265_RS03055 at 759.440 kb on + strand, within CA265_RS03055 at 759.441 kb on - strand, within CA265_RS03055 at 759.447 kb on + strand, within CA265_RS03055 at 759.486 kb on + strand, within CA265_RS03055 at 759.490 kb on - strand, within CA265_RS03055 at 759.576 kb on + strand at 759.577 kb on - strand at 759.577 kb on - strand at 759.644 kb on + strand at 759.712 kb on + strand, within CA265_RS03060 at 759.712 kb on + strand, within CA265_RS03060 at 759.820 kb on + strand, within CA265_RS03060 at 759.821 kb on - strand, within CA265_RS03060 at 760.008 kb on + strand, within CA265_RS03060 at 760.059 kb on - strand, within CA265_RS03060 at 760.076 kb on + strand, within CA265_RS03060 at 760.077 kb on - strand, within CA265_RS03060 at 760.079 kb on + strand, within CA265_RS03060 at 760.080 kb on - strand, within CA265_RS03060 at 760.080 kb on - strand, within CA265_RS03060 at 760.080 kb on - strand, within CA265_RS03060 at 760.082 kb on + strand, within CA265_RS03060 at 760.082 kb on + strand, within CA265_RS03060 at 760.083 kb on - strand, within CA265_RS03060 at 760.107 kb on - strand, within CA265_RS03060 at 760.143 kb on + strand, within CA265_RS03060 at 760.203 kb on + strand, within CA265_RS03060 at 760.219 kb on - strand, within CA265_RS03060 at 760.220 kb on + strand, within CA265_RS03060 at 760.266 kb on + strand at 760.267 kb on - strand at 760.287 kb on + strand at 760.296 kb on + strand at 760.296 kb on + strand at 760.317 kb on - strand at 760.419 kb on - strand at 760.453 kb on - strand at 760.473 kb on - strand at 760.473 kb on - strand at 760.477 kb on + strand at 760.478 kb on - strand at 760.525 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.025 mg/ml remove 757,288 + CA265_RS03045 0.72 +1.0 757,344 + CA265_RS03045 0.78 -0.9 757,344 + CA265_RS03045 0.78 -0.1 757,345 - CA265_RS03045 0.78 -1.6 757,360 - CA265_RS03045 0.79 -0.4 757,451 + CA265_RS03045 0.89 +0.6 757,453 + CA265_RS03045 0.89 -0.1 757,454 - CA265_RS03045 0.89 -0.7 757,457 - CA265_RS03045 0.90 -0.3 757,464 + -0.9 757,465 - -0.0 757,468 + +0.3 757,518 + +1.2 757,519 - -0.2 757,519 - -0.3 757,521 - -0.1 757,521 - +1.0 757,707 + CA265_RS03050 0.26 -0.4 757,712 + CA265_RS03050 0.27 -1.0 757,713 - CA265_RS03050 0.28 -0.3 757,713 - CA265_RS03050 0.28 +1.2 757,716 + CA265_RS03050 0.28 -0.0 757,748 + CA265_RS03050 0.35 -0.9 757,803 + CA265_RS03050 0.46 +0.8 757,817 + CA265_RS03050 0.48 -0.2 757,931 - CA265_RS03050 0.71 +2.2 757,935 + CA265_RS03050 0.72 +0.9 757,936 - CA265_RS03050 0.72 +1.0 757,959 + CA265_RS03050 0.77 +0.5 758,060 - +0.9 758,079 + -0.6 758,080 - +0.5 758,200 - -1.3 758,200 - +1.1 758,207 + -0.2 758,230 + -0.8 758,231 - +0.5 758,277 - +1.1 758,338 + +0.2 758,343 + +0.2 758,353 + +0.5 758,365 - +0.4 758,380 + +0.7 758,380 + +0.2 758,394 - +0.0 758,427 - CA265_RS03055 0.11 +0.2 758,427 - CA265_RS03055 0.11 -0.4 758,440 + CA265_RS03055 0.12 -0.4 758,441 - CA265_RS03055 0.12 -1.2 758,442 + CA265_RS03055 0.12 +0.2 758,443 - CA265_RS03055 0.12 +0.6 758,452 - CA265_RS03055 0.13 +0.1 758,485 + CA265_RS03055 0.16 +0.1 758,503 - CA265_RS03055 0.17 -0.9 758,520 + CA265_RS03055 0.18 -2.0 758,523 + CA265_RS03055 0.18 +0.4 758,524 - CA265_RS03055 0.18 -0.8 758,554 - CA265_RS03055 0.21 -0.1 758,554 - CA265_RS03055 0.21 +0.6 758,571 + CA265_RS03055 0.22 -0.6 758,620 + CA265_RS03055 0.25 +0.7 758,621 - CA265_RS03055 0.26 -0.3 758,646 + CA265_RS03055 0.27 +0.8 758,647 - CA265_RS03055 0.27 +1.0 758,697 + CA265_RS03055 0.31 +0.5 758,725 + CA265_RS03055 0.33 -0.0 758,725 + CA265_RS03055 0.33 +0.7 758,793 + CA265_RS03055 0.38 +1.4 758,794 - CA265_RS03055 0.38 +0.4 758,800 + CA265_RS03055 0.39 +0.5 758,800 + CA265_RS03055 0.39 +0.3 758,896 - CA265_RS03055 0.46 +0.9 758,914 + CA265_RS03055 0.47 -0.4 758,914 + CA265_RS03055 0.47 +0.8 758,915 - CA265_RS03055 0.47 -0.2 758,937 + CA265_RS03055 0.49 +0.6 759,037 + CA265_RS03055 0.56 -0.2 759,038 - CA265_RS03055 0.56 +0.4 759,072 + CA265_RS03055 0.59 -0.7 759,085 + CA265_RS03055 0.60 -0.2 759,103 + CA265_RS03055 0.61 +1.0 759,111 - CA265_RS03055 0.62 +0.3 759,115 - CA265_RS03055 0.62 -0.5 759,143 - CA265_RS03055 0.64 -1.3 759,238 - CA265_RS03055 0.71 -0.2 759,304 + CA265_RS03055 0.76 +1.9 759,382 + CA265_RS03055 0.82 +0.5 759,383 - CA265_RS03055 0.82 -1.5 759,383 - CA265_RS03055 0.82 +0.3 759,390 - CA265_RS03055 0.82 +1.0 759,440 + CA265_RS03055 0.86 -1.0 759,440 + CA265_RS03055 0.86 +1.9 759,441 - CA265_RS03055 0.86 -0.5 759,447 + CA265_RS03055 0.87 -1.4 759,486 + CA265_RS03055 0.90 -0.1 759,490 - CA265_RS03055 0.90 -0.3 759,576 + -0.0 759,577 - +0.4 759,577 - -0.9 759,644 + +0.3 759,712 + CA265_RS03060 0.13 -0.0 759,712 + CA265_RS03060 0.13 -1.4 759,820 + CA265_RS03060 0.28 -1.1 759,821 - CA265_RS03060 0.29 -0.6 760,008 + CA265_RS03060 0.56 -0.5 760,059 - CA265_RS03060 0.64 -0.2 760,076 + CA265_RS03060 0.66 -3.0 760,077 - CA265_RS03060 0.66 -0.3 760,079 + CA265_RS03060 0.67 -0.1 760,080 - CA265_RS03060 0.67 -0.6 760,080 - CA265_RS03060 0.67 -0.1 760,080 - CA265_RS03060 0.67 -0.8 760,082 + CA265_RS03060 0.67 -1.0 760,082 + CA265_RS03060 0.67 -0.6 760,083 - CA265_RS03060 0.67 -0.8 760,107 - CA265_RS03060 0.71 -1.1 760,143 + CA265_RS03060 0.76 +0.9 760,203 + CA265_RS03060 0.85 -1.1 760,219 - CA265_RS03060 0.87 -0.3 760,220 + CA265_RS03060 0.87 +0.3 760,266 + +0.6 760,267 - +0.6 760,287 + -0.1 760,296 + +0.3 760,296 + -0.4 760,317 - +0.2 760,419 - +0.5 760,453 - -1.2 760,473 - -1.3 760,473 - -0.8 760,477 + -1.0 760,478 - +0.2 760,525 + -0.0
Or see this region's nucleotide sequence