Experiment: R2A with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01555 and CA265_RS01560 are separated by 0 nucleotides CA265_RS01560 and CA265_RS01565 are separated by 2 nucleotides CA265_RS01565 and CA265_RS01570 are separated by 3 nucleotides CA265_RS01570 and CA265_RS01575 are separated by 15 nucleotides
CA265_RS01555: CA265_RS01555 - KaiC 1, at 400,883 to 402,631
_RS01555
CA265_RS01560: CA265_RS01560 - circadian clock protein KaiB, at 402,632 to 402,922
_RS01560
CA265_RS01565: CA265_RS01565 - circadian clock protein KaiB, at 402,925 to 403,239
_RS01565
CA265_RS01570: CA265_RS01570 - hypothetical protein, at 403,243 to 405,012
_RS01570
CA265_RS01575: CA265_RS01575 - hypothetical protein, at 405,028 to 405,777
_RS01575
Position (kb) 403
404
405
406 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 402.316 kb on - strand, within CA265_RS01555 at 402.316 kb on - strand, within CA265_RS01555 at 402.318 kb on - strand, within CA265_RS01555 at 402.318 kb on - strand, within CA265_RS01555 at 402.437 kb on - strand, within CA265_RS01555 at 402.508 kb on + strand at 402.520 kb on - strand at 402.520 kb on - strand at 402.590 kb on - strand at 402.662 kb on + strand, within CA265_RS01560 at 402.662 kb on + strand, within CA265_RS01560 at 402.743 kb on + strand, within CA265_RS01560 at 402.745 kb on + strand, within CA265_RS01560 at 402.898 kb on + strand at 402.899 kb on - strand at 402.900 kb on + strand at 402.905 kb on - strand at 402.921 kb on - strand at 402.921 kb on - strand at 402.954 kb on + strand at 403.032 kb on + strand, within CA265_RS01565 at 403.033 kb on - strand, within CA265_RS01565 at 403.073 kb on - strand, within CA265_RS01565 at 403.110 kb on - strand, within CA265_RS01565 at 403.116 kb on - strand, within CA265_RS01565 at 403.263 kb on + strand at 403.263 kb on + strand at 403.264 kb on - strand at 403.282 kb on + strand at 403.282 kb on + strand at 403.365 kb on - strand at 403.365 kb on - strand at 403.413 kb on + strand at 403.413 kb on + strand at 403.484 kb on - strand, within CA265_RS01570 at 403.517 kb on + strand, within CA265_RS01570 at 403.583 kb on + strand, within CA265_RS01570 at 403.584 kb on - strand, within CA265_RS01570 at 403.596 kb on + strand, within CA265_RS01570 at 403.597 kb on - strand, within CA265_RS01570 at 403.597 kb on - strand, within CA265_RS01570 at 403.599 kb on + strand, within CA265_RS01570 at 403.618 kb on - strand, within CA265_RS01570 at 403.618 kb on - strand, within CA265_RS01570 at 403.618 kb on - strand, within CA265_RS01570 at 403.763 kb on + strand, within CA265_RS01570 at 403.943 kb on + strand, within CA265_RS01570 at 403.959 kb on + strand, within CA265_RS01570 at 404.154 kb on + strand, within CA265_RS01570 at 404.179 kb on + strand, within CA265_RS01570 at 404.203 kb on - strand, within CA265_RS01570 at 404.231 kb on + strand, within CA265_RS01570 at 404.232 kb on - strand, within CA265_RS01570 at 404.278 kb on + strand, within CA265_RS01570 at 404.279 kb on - strand, within CA265_RS01570 at 404.297 kb on + strand, within CA265_RS01570 at 404.298 kb on - strand, within CA265_RS01570 at 404.298 kb on - strand, within CA265_RS01570 at 404.593 kb on + strand, within CA265_RS01570 at 404.593 kb on + strand, within CA265_RS01570 at 404.593 kb on + strand, within CA265_RS01570 at 404.594 kb on - strand, within CA265_RS01570 at 404.594 kb on - strand, within CA265_RS01570 at 404.595 kb on + strand, within CA265_RS01570 at 404.596 kb on - strand, within CA265_RS01570 at 404.616 kb on + strand, within CA265_RS01570 at 404.736 kb on - strand, within CA265_RS01570 at 404.771 kb on + strand, within CA265_RS01570 at 404.896 kb on - strand at 404.976 kb on + strand at 404.977 kb on - strand at 404.977 kb on - strand at 405.043 kb on + strand at 405.142 kb on - strand, within CA265_RS01575 at 405.143 kb on + strand, within CA265_RS01575 at 405.199 kb on + strand, within CA265_RS01575 at 405.200 kb on - strand, within CA265_RS01575 at 405.235 kb on + strand, within CA265_RS01575 at 405.236 kb on - strand, within CA265_RS01575 at 405.239 kb on - strand, within CA265_RS01575 at 405.269 kb on - strand, within CA265_RS01575 at 405.301 kb on - strand, within CA265_RS01575 at 405.359 kb on - strand, within CA265_RS01575 at 405.360 kb on + strand, within CA265_RS01575 at 405.360 kb on + strand, within CA265_RS01575 at 405.360 kb on + strand, within CA265_RS01575 at 405.361 kb on - strand, within CA265_RS01575 at 405.362 kb on + strand, within CA265_RS01575 at 405.363 kb on - strand, within CA265_RS01575 at 405.366 kb on + strand at 405.366 kb on + strand, within CA265_RS01575 at 405.408 kb on + strand, within CA265_RS01575 at 405.409 kb on - strand, within CA265_RS01575 at 405.422 kb on + strand, within CA265_RS01575 at 405.475 kb on - strand, within CA265_RS01575 at 405.588 kb on + strand, within CA265_RS01575 at 405.604 kb on - strand, within CA265_RS01575 at 405.639 kb on + strand, within CA265_RS01575 at 405.650 kb on + strand, within CA265_RS01575 at 405.651 kb on - strand, within CA265_RS01575 at 405.651 kb on - strand, within CA265_RS01575 at 405.748 kb on + strand at 405.816 kb on + strand at 405.909 kb on + strand at 405.959 kb on + strand at 406.012 kb on + strand at 406.012 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.025 mg/ml remove 402,316 - CA265_RS01555 0.82 -0.0 402,316 - CA265_RS01555 0.82 +0.3 402,318 - CA265_RS01555 0.82 -0.3 402,318 - CA265_RS01555 0.82 +1.2 402,437 - CA265_RS01555 0.89 -0.9 402,508 + -0.6 402,520 - +0.2 402,520 - +0.5 402,590 - -0.7 402,662 + CA265_RS01560 0.10 -0.7 402,662 + CA265_RS01560 0.10 -0.3 402,743 + CA265_RS01560 0.38 -0.3 402,745 + CA265_RS01560 0.39 +1.0 402,898 + +0.8 402,899 - +0.4 402,900 + +0.4 402,905 - -0.1 402,921 - +0.1 402,921 - +0.2 402,954 + -1.1 403,032 + CA265_RS01565 0.34 -0.4 403,033 - CA265_RS01565 0.34 +0.6 403,073 - CA265_RS01565 0.47 +0.0 403,110 - CA265_RS01565 0.59 +1.3 403,116 - CA265_RS01565 0.61 +0.6 403,263 + +0.0 403,263 + +0.7 403,264 - +0.9 403,282 + +0.1 403,282 + -0.5 403,365 - +0.4 403,365 - -1.2 403,413 + +0.4 403,413 + -1.5 403,484 - CA265_RS01570 0.14 -0.0 403,517 + CA265_RS01570 0.15 +0.5 403,583 + CA265_RS01570 0.19 -0.5 403,584 - CA265_RS01570 0.19 +0.3 403,596 + CA265_RS01570 0.20 +0.3 403,597 - CA265_RS01570 0.20 +0.3 403,597 - CA265_RS01570 0.20 -0.5 403,599 + CA265_RS01570 0.20 -1.2 403,618 - CA265_RS01570 0.21 -0.7 403,618 - CA265_RS01570 0.21 +0.3 403,618 - CA265_RS01570 0.21 -0.5 403,763 + CA265_RS01570 0.29 -0.5 403,943 + CA265_RS01570 0.40 -0.5 403,959 + CA265_RS01570 0.40 -0.1 404,154 + CA265_RS01570 0.51 +0.0 404,179 + CA265_RS01570 0.53 -0.7 404,203 - CA265_RS01570 0.54 +0.1 404,231 + CA265_RS01570 0.56 -0.2 404,232 - CA265_RS01570 0.56 -3.3 404,278 + CA265_RS01570 0.58 -1.2 404,279 - CA265_RS01570 0.59 -0.9 404,297 + CA265_RS01570 0.60 -1.1 404,298 - CA265_RS01570 0.60 +0.4 404,298 - CA265_RS01570 0.60 -1.2 404,593 + CA265_RS01570 0.76 -1.8 404,593 + CA265_RS01570 0.76 +0.5 404,593 + CA265_RS01570 0.76 +0.0 404,594 - CA265_RS01570 0.76 -0.7 404,594 - CA265_RS01570 0.76 -0.8 404,595 + CA265_RS01570 0.76 -1.1 404,596 - CA265_RS01570 0.76 -0.5 404,616 + CA265_RS01570 0.78 -0.1 404,736 - CA265_RS01570 0.84 -1.9 404,771 + CA265_RS01570 0.86 +0.1 404,896 - -1.2 404,976 + -0.1 404,977 - +0.3 404,977 - -0.2 405,043 + +0.1 405,142 - CA265_RS01575 0.15 +0.6 405,143 + CA265_RS01575 0.15 -0.2 405,199 + CA265_RS01575 0.23 -2.0 405,200 - CA265_RS01575 0.23 -0.5 405,235 + CA265_RS01575 0.28 +1.5 405,236 - CA265_RS01575 0.28 -1.6 405,239 - CA265_RS01575 0.28 -0.6 405,269 - CA265_RS01575 0.32 +0.3 405,301 - CA265_RS01575 0.36 +0.1 405,359 - CA265_RS01575 0.44 -0.3 405,360 + CA265_RS01575 0.44 -0.4 405,360 + CA265_RS01575 0.44 -0.4 405,360 + CA265_RS01575 0.44 -0.2 405,361 - CA265_RS01575 0.44 -0.6 405,362 + CA265_RS01575 0.45 -0.6 405,363 - CA265_RS01575 0.45 -0.9 405,366 + -0.1 405,366 + CA265_RS01575 0.45 +0.5 405,408 + CA265_RS01575 0.51 +1.3 405,409 - CA265_RS01575 0.51 -1.9 405,422 + CA265_RS01575 0.53 +0.5 405,475 - CA265_RS01575 0.60 -0.6 405,588 + CA265_RS01575 0.75 +1.3 405,604 - CA265_RS01575 0.77 +0.7 405,639 + CA265_RS01575 0.81 +0.9 405,650 + CA265_RS01575 0.83 -1.1 405,651 - CA265_RS01575 0.83 +0.7 405,651 - CA265_RS01575 0.83 -0.1 405,748 + -0.1 405,816 + -0.9 405,909 + -0.4 405,959 + +0.6 406,012 + +0.0 406,012 + +0.5
Or see this region's nucleotide sequence