Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3150 and Shewana3_3151 are separated by 222 nucleotides Shewana3_3151 and Shewana3_3152 overlap by 8 nucleotides Shewana3_3152 and Shewana3_3153 are separated by 44 nucleotides
Shewana3_3150: Shewana3_3150 - hemerythrin HHE cation binding domain-containing protein (RefSeq), at 3,778,435 to 3,778,971
_3150
Shewana3_3151: Shewana3_3151 - hypothetical protein (RefSeq), at 3,779,194 to 3,779,901
_3151
Shewana3_3152: Shewana3_3152 - ECF subfamily RNA polymerase sigma-24 factor (RefSeq), at 3,779,894 to 3,780,496
_3152
Shewana3_3153: Shewana3_3153 - von Willebrand factor, type A (RefSeq), at 3,780,541 to 3,782,382
_3153
Position (kb)
3779
3780
3781 Strain fitness (log2 ratio)
-2
-1
0
1 at 3779.411 kb on - strand, within Shewana3_3151 at 3779.884 kb on - strand at 3779.950 kb on - strand at 3780.065 kb on + strand, within Shewana3_3152 at 3780.092 kb on - strand, within Shewana3_3152 at 3780.137 kb on - strand, within Shewana3_3152 at 3780.255 kb on + strand, within Shewana3_3152 at 3780.296 kb on + strand, within Shewana3_3152 at 3780.304 kb on - strand, within Shewana3_3152 at 3780.448 kb on + strand at 3780.485 kb on + strand at 3780.709 kb on - strand at 3780.773 kb on - strand, within Shewana3_3153 at 3780.995 kb on + strand, within Shewana3_3153 at 3781.307 kb on - strand, within Shewana3_3153 at 3781.321 kb on - strand, within Shewana3_3153
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,779,411 - Shewana3_3151 0.31 -1.7 3,779,884 - +1.5 3,779,950 - +0.2 3,780,065 + Shewana3_3152 0.28 -0.7 3,780,092 - Shewana3_3152 0.33 +0.2 3,780,137 - Shewana3_3152 0.40 -2.4 3,780,255 + Shewana3_3152 0.60 -2.0 3,780,296 + Shewana3_3152 0.67 -0.6 3,780,304 - Shewana3_3152 0.68 -1.7 3,780,448 + -2.6 3,780,485 + +0.0 3,780,709 - -0.9 3,780,773 - Shewana3_3153 0.13 -2.3 3,780,995 + Shewana3_3153 0.25 +0.7 3,781,307 - Shewana3_3153 0.42 +0.3 3,781,321 - Shewana3_3153 0.42 +0.2
Or see this region's nucleotide sequence