Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2969 and Shewana3_2970 are separated by 246 nucleotides Shewana3_2970 and Shewana3_2971 are separated by 255 nucleotides Shewana3_2971 and Shewana3_2972 are separated by 20 nucleotides
Shewana3_2969: Shewana3_2969 - transglutaminase domain-containing protein (RefSeq), at 3,569,325 to 3,570,464
_2969
Shewana3_2970: Shewana3_2970 - HAAAP family transport protein (RefSeq), at 3,570,711 to 3,571,982
_2970
Shewana3_2971: Shewana3_2971 - putative endoribonuclease L-PSP (RefSeq), at 3,572,238 to 3,572,621
_2971
Shewana3_2972: Shewana3_2972 - hypothetical protein (RefSeq), at 3,572,642 to 3,573,916
_2972
Position (kb)
3570
3571
3572 Strain fitness (log2 ratio)
-2
-1
0
1 at 3569.878 kb on + strand, within Shewana3_2969 at 3569.992 kb on + strand, within Shewana3_2969 at 3570.365 kb on - strand at 3570.366 kb on + strand at 3570.585 kb on - strand at 3570.592 kb on - strand at 3570.744 kb on + strand at 3570.835 kb on - strand at 3570.835 kb on - strand at 3570.915 kb on - strand, within Shewana3_2970 at 3571.091 kb on + strand, within Shewana3_2970 at 3571.099 kb on - strand, within Shewana3_2970 at 3571.099 kb on - strand, within Shewana3_2970 at 3571.209 kb on - strand, within Shewana3_2970 at 3571.316 kb on + strand, within Shewana3_2970 at 3571.406 kb on - strand, within Shewana3_2970 at 3571.447 kb on - strand, within Shewana3_2970 at 3571.481 kb on + strand, within Shewana3_2970 at 3571.550 kb on + strand, within Shewana3_2970 at 3571.983 kb on + strand at 3572.266 kb on + strand at 3572.364 kb on + strand, within Shewana3_2971 at 3572.696 kb on + strand at 3572.697 kb on + strand at 3572.820 kb on + strand, within Shewana3_2972
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,569,878 + Shewana3_2969 0.49 +0.3 3,569,992 + Shewana3_2969 0.59 -0.1 3,570,365 - +0.1 3,570,366 + -0.1 3,570,585 - +0.1 3,570,592 - -1.6 3,570,744 + +0.3 3,570,835 - -2.1 3,570,835 - +0.5 3,570,915 - Shewana3_2970 0.16 +0.8 3,571,091 + Shewana3_2970 0.30 +0.1 3,571,099 - Shewana3_2970 0.31 -0.0 3,571,099 - Shewana3_2970 0.31 -0.1 3,571,209 - Shewana3_2970 0.39 +0.5 3,571,316 + Shewana3_2970 0.48 -1.8 3,571,406 - Shewana3_2970 0.55 +0.6 3,571,447 - Shewana3_2970 0.58 -1.6 3,571,481 + Shewana3_2970 0.61 +0.4 3,571,550 + Shewana3_2970 0.66 +0.4 3,571,983 + -2.0 3,572,266 + +0.6 3,572,364 + Shewana3_2971 0.33 +0.2 3,572,696 + -1.6 3,572,697 + -1.9 3,572,820 + Shewana3_2972 0.14 -0.2
Or see this region's nucleotide sequence