Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2956 and Shewana3_2957 are separated by 7 nucleotides Shewana3_2957 and Shewana3_2958 are separated by 44 nucleotides Shewana3_2958 and Shewana3_2959 overlap by 1 nucleotides
Shewana3_2956: Shewana3_2956 - hypothetical protein (RefSeq), at 3,549,897 to 3,550,760
_2956
Shewana3_2957: Shewana3_2957 - HNH endonuclease (RefSeq), at 3,550,768 to 3,551,529
_2957
Shewana3_2958: Shewana3_2958 - hypothetical protein (RefSeq), at 3,551,574 to 3,552,383
_2958
Shewana3_2959: Shewana3_2959 - hypothetical protein (RefSeq), at 3,552,383 to 3,553,984
_2959
Position (kb)
3551
3552
3553 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3550.589 kb on + strand, within Shewana3_2956 at 3550.590 kb on + strand, within Shewana3_2956 at 3550.672 kb on + strand, within Shewana3_2956 at 3550.713 kb on - strand at 3551.018 kb on + strand, within Shewana3_2957 at 3551.389 kb on - strand, within Shewana3_2957 at 3551.482 kb on - strand at 3551.519 kb on - strand at 3551.519 kb on - strand at 3551.940 kb on - strand, within Shewana3_2958 at 3552.141 kb on - strand, within Shewana3_2958 at 3552.307 kb on + strand at 3552.315 kb on - strand at 3552.353 kb on + strand at 3552.486 kb on - strand at 3552.666 kb on + strand, within Shewana3_2959 at 3552.775 kb on + strand, within Shewana3_2959 at 3552.780 kb on + strand, within Shewana3_2959 at 3552.878 kb on - strand, within Shewana3_2959 at 3553.074 kb on + strand, within Shewana3_2959 at 3553.129 kb on - strand, within Shewana3_2959 at 3553.311 kb on - strand, within Shewana3_2959 at 3553.372 kb on - strand, within Shewana3_2959
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,550,589 + Shewana3_2956 0.80 -2.1 3,550,590 + Shewana3_2956 0.80 +0.4 3,550,672 + Shewana3_2956 0.90 +1.8 3,550,713 - -2.6 3,551,018 + Shewana3_2957 0.33 +0.7 3,551,389 - Shewana3_2957 0.81 +0.4 3,551,482 - -0.6 3,551,519 - +0.4 3,551,519 - +0.5 3,551,940 - Shewana3_2958 0.45 +0.1 3,552,141 - Shewana3_2958 0.70 -2.1 3,552,307 + -0.5 3,552,315 - +0.8 3,552,353 + -1.9 3,552,486 - +0.3 3,552,666 + Shewana3_2959 0.18 +0.8 3,552,775 + Shewana3_2959 0.24 +0.2 3,552,780 + Shewana3_2959 0.25 +0.2 3,552,878 - Shewana3_2959 0.31 -1.8 3,553,074 + Shewana3_2959 0.43 +0.1 3,553,129 - Shewana3_2959 0.47 +0.9 3,553,311 - Shewana3_2959 0.58 -2.0 3,553,372 - Shewana3_2959 0.62 -2.9
Or see this region's nucleotide sequence