Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2764 and fabG are separated by 207 nucleotides fabG and Shewana3_2766 are separated by 19 nucleotides Shewana3_2766 and Shewana3_2767 are separated by 100 nucleotides
Shewana3_2764: Shewana3_2764 - hypothetical protein (RefSeq), at 3,303,121 to 3,303,363
_2764
Shewana3_2765: fabG - 3-ketoacyl-(acyl-carrier-protein) reductase (RefSeq), at 3,303,571 to 3,304,329
fabG
Shewana3_2766: Shewana3_2766 - 3-hydroxyisobutyrate dehydrogenase (RefSeq), at 3,304,349 to 3,305,251
_2766
Shewana3_2767: Shewana3_2767 - enoyl-CoA hydratase/isomerase (RefSeq), at 3,305,352 to 3,306,503
_2767
Position (kb)
3304
3305
3306 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3303.392 kb on - strand at 3303.642 kb on + strand at 3303.750 kb on + strand, within fabG at 3303.758 kb on - strand, within fabG at 3304.841 kb on + strand, within Shewana3_2766 at 3304.849 kb on - strand, within Shewana3_2766 at 3304.907 kb on + strand, within Shewana3_2766 at 3304.908 kb on + strand, within Shewana3_2766 at 3304.980 kb on - strand, within Shewana3_2766 at 3305.067 kb on - strand, within Shewana3_2766 at 3305.110 kb on + strand, within Shewana3_2766 at 3305.143 kb on - strand, within Shewana3_2766 at 3305.247 kb on - strand at 3305.416 kb on - strand at 3305.471 kb on - strand, within Shewana3_2767 at 3305.510 kb on + strand, within Shewana3_2767 at 3305.675 kb on - strand, within Shewana3_2767 at 3305.704 kb on - strand, within Shewana3_2767 at 3305.873 kb on - strand, within Shewana3_2767 at 3305.978 kb on + strand, within Shewana3_2767 at 3305.986 kb on - strand, within Shewana3_2767 at 3306.124 kb on + strand, within Shewana3_2767 at 3306.124 kb on + strand, within Shewana3_2767
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,303,392 - +0.4 3,303,642 + -3.1 3,303,750 + fabG Shewana3_2765 0.24 -2.6 3,303,758 - fabG Shewana3_2765 0.25 -1.2 3,304,841 + Shewana3_2766 0.54 -5.2 3,304,849 - Shewana3_2766 0.55 -1.1 3,304,907 + Shewana3_2766 0.62 -4.3 3,304,908 + Shewana3_2766 0.62 -4.9 3,304,980 - Shewana3_2766 0.70 -4.3 3,305,067 - Shewana3_2766 0.80 -3.0 3,305,110 + Shewana3_2766 0.84 -2.6 3,305,143 - Shewana3_2766 0.88 -2.2 3,305,247 - -3.5 3,305,416 - -4.9 3,305,471 - Shewana3_2767 0.10 -5.2 3,305,510 + Shewana3_2767 0.14 -4.8 3,305,675 - Shewana3_2767 0.28 -4.5 3,305,704 - Shewana3_2767 0.31 -4.3 3,305,873 - Shewana3_2767 0.45 -3.0 3,305,978 + Shewana3_2767 0.54 -4.2 3,305,986 - Shewana3_2767 0.55 -4.9 3,306,124 + Shewana3_2767 0.67 -5.5 3,306,124 + Shewana3_2767 0.67 -4.3
Or see this region's nucleotide sequence