Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2655 and Shewana3_2656 are separated by 118 nucleotides Shewana3_2656 and Shewana3_2657 are separated by 149 nucleotides Shewana3_2657 and Shewana3_2658 are separated by 226 nucleotides Shewana3_2658 and Shewana3_2659 are separated by 260 nucleotides
Shewana3_2655: Shewana3_2655 - trans-2-enoyl-CoA reductase (RefSeq), at 3,168,667 to 3,169,869
_2655
Shewana3_2656: Shewana3_2656 - molybdenum-pterin binding (RefSeq), at 3,169,988 to 3,170,200
_2656
Shewana3_2657: Shewana3_2657 - PpiC-type peptidyl-prolyl cis-trans isomerase (RefSeq), at 3,170,350 to 3,172,215
_2657
Shewana3_2658: Shewana3_2658 - histone family protein DNA-binding protein (RefSeq), at 3,172,442 to 3,172,714
_2658
Shewana3_2659: Shewana3_2659 - Lon-A peptidase (RefSeq), at 3,172,975 to 3,175,332
_2659
Position (kb)
3170
3171
3172
3173 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3170.087 kb on + strand, within Shewana3_2656 at 3170.095 kb on - strand, within Shewana3_2656 at 3170.095 kb on - strand, within Shewana3_2656 at 3170.850 kb on - strand, within Shewana3_2657 at 3170.896 kb on - strand, within Shewana3_2657 at 3170.950 kb on - strand, within Shewana3_2657 at 3171.187 kb on - strand, within Shewana3_2657 at 3171.426 kb on - strand, within Shewana3_2657 at 3171.615 kb on - strand, within Shewana3_2657 at 3172.025 kb on - strand, within Shewana3_2657 at 3172.897 kb on + strand at 3172.905 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,170,087 + Shewana3_2656 0.46 -1.8 3,170,095 - Shewana3_2656 0.50 +0.9 3,170,095 - Shewana3_2656 0.50 -0.4 3,170,850 - Shewana3_2657 0.27 +1.5 3,170,896 - Shewana3_2657 0.29 +0.3 3,170,950 - Shewana3_2657 0.32 +0.3 3,171,187 - Shewana3_2657 0.45 +0.5 3,171,426 - Shewana3_2657 0.58 +0.7 3,171,615 - Shewana3_2657 0.68 +1.5 3,172,025 - Shewana3_2657 0.90 -0.5 3,172,897 + -3.0 3,172,905 - -1.7
Or see this region's nucleotide sequence