Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2425 and Shewana3_2426 are separated by 138 nucleotides Shewana3_2426 and Shewana3_2427 are separated by 77 nucleotides Shewana3_2427 and Shewana3_2428 are separated by 66 nucleotides
Shewana3_2425: Shewana3_2425 - di-haem cytochrome c peroxidase (RefSeq), at 2,907,852 to 2,908,853
_2425
Shewana3_2426: Shewana3_2426 - nucleoid-associated protein NdpA (RefSeq), at 2,908,992 to 2,910,020
_2426
Shewana3_2427: Shewana3_2427 - hypothetical protein (RefSeq), at 2,910,098 to 2,910,316
_2427
Shewana3_2428: Shewana3_2428 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (RefSeq), at 2,910,383 to 2,912,173
_2428
Position (kb)
2908
2909
2910
2911 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2908.123 kb on - strand, within Shewana3_2425 at 2908.180 kb on + strand, within Shewana3_2425 at 2908.292 kb on + strand, within Shewana3_2425 at 2908.420 kb on - strand, within Shewana3_2425 at 2908.537 kb on + strand, within Shewana3_2425 at 2908.537 kb on + strand, within Shewana3_2425 at 2908.546 kb on - strand, within Shewana3_2425 at 2908.548 kb on + strand, within Shewana3_2425 at 2908.613 kb on + strand, within Shewana3_2425 at 2909.156 kb on + strand, within Shewana3_2426 at 2909.329 kb on - strand, within Shewana3_2426 at 2909.384 kb on - strand, within Shewana3_2426 at 2909.424 kb on - strand, within Shewana3_2426 at 2909.821 kb on + strand, within Shewana3_2426 at 2909.884 kb on + strand, within Shewana3_2426 at 2909.907 kb on + strand, within Shewana3_2426 at 2910.054 kb on + strand at 2910.669 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,908,123 - Shewana3_2425 0.27 +0.1 2,908,180 + Shewana3_2425 0.33 -1.8 2,908,292 + Shewana3_2425 0.44 +0.1 2,908,420 - Shewana3_2425 0.57 +0.3 2,908,537 + Shewana3_2425 0.68 +0.3 2,908,537 + Shewana3_2425 0.68 +0.6 2,908,546 - Shewana3_2425 0.69 -0.1 2,908,548 + Shewana3_2425 0.69 +0.0 2,908,613 + Shewana3_2425 0.76 +1.0 2,909,156 + Shewana3_2426 0.16 +1.0 2,909,329 - Shewana3_2426 0.33 +0.3 2,909,384 - Shewana3_2426 0.38 -2.3 2,909,424 - Shewana3_2426 0.42 -1.4 2,909,821 + Shewana3_2426 0.81 -0.3 2,909,884 + Shewana3_2426 0.87 -2.6 2,909,907 + Shewana3_2426 0.89 -3.7 2,910,054 + -0.4 2,910,669 + -1.5
Or see this region's nucleotide sequence