Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1897 and Shewana3_1898 are separated by 704 nucleotides Shewana3_1898 and Shewana3_1899 are separated by 44 nucleotides Shewana3_1899 and Shewana3_1900 are separated by 2 nucleotides
Shewana3_1897: Shewana3_1897 - ATP-dependent helicase HrpA (RefSeq), at 2,250,421 to 2,254,302
_1897
Shewana3_1898: Shewana3_1898 - hypothetical protein (RefSeq), at 2,255,007 to 2,255,165
_1898
Shewana3_1899: Shewana3_1899 - NapD family protein (RefSeq), at 2,255,210 to 2,255,467
_1899
Shewana3_1900: Shewana3_1900 - nitrate reductase catalytic subunit (RefSeq), at 2,255,470 to 2,257,959
_1900
Position (kb)
2255
2256 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2254.040 kb on + strand at 2254.258 kb on - strand at 2254.594 kb on + strand at 2254.663 kb on - strand at 2254.901 kb on + strand at 2254.901 kb on + strand at 2254.964 kb on + strand at 2255.067 kb on - strand, within Shewana3_1898 at 2255.081 kb on + strand, within Shewana3_1898 at 2255.081 kb on + strand, within Shewana3_1898 at 2255.315 kb on + strand, within Shewana3_1899 at 2255.575 kb on - strand at 2255.575 kb on - strand at 2255.599 kb on + strand at 2255.607 kb on - strand at 2255.773 kb on - strand, within Shewana3_1900 at 2256.011 kb on - strand, within Shewana3_1900
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 2,254,040 + +0.2 2,254,258 - -0.4 2,254,594 + -2.9 2,254,663 - -0.1 2,254,901 + +1.1 2,254,901 + -2.7 2,254,964 + +0.3 2,255,067 - Shewana3_1898 0.38 +0.7 2,255,081 + Shewana3_1898 0.47 +1.0 2,255,081 + Shewana3_1898 0.47 +0.6 2,255,315 + Shewana3_1899 0.41 +0.0 2,255,575 - -0.4 2,255,575 - -0.0 2,255,599 + -0.4 2,255,607 - -0.6 2,255,773 - Shewana3_1900 0.12 -0.1 2,256,011 - Shewana3_1900 0.22 -3.1
Or see this region's nucleotide sequence