Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0111 and Shewana3_0112 are separated by 117 nucleotides Shewana3_0112 and Shewana3_0113 are separated by 385 nucleotides
Shewana3_0111: Shewana3_0111 - peptidase M48, Ste24p (RefSeq), at 127,143 to 128,486
_0111
Shewana3_0112: Shewana3_0112 - hypothetical protein (RefSeq), at 128,604 to 129,218
_0112
Shewana3_0113: Shewana3_0113 - hypothetical protein (RefSeq), at 129,604 to 130,224
_0113
Position (kb) 128
129
130 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 127.666 kb on - strand, within Shewana3_0111 at 127.721 kb on + strand, within Shewana3_0111 at 127.756 kb on - strand, within Shewana3_0111 at 127.767 kb on - strand, within Shewana3_0111 at 127.807 kb on - strand, within Shewana3_0111 at 127.855 kb on - strand, within Shewana3_0111 at 127.892 kb on - strand, within Shewana3_0111 at 127.947 kb on + strand, within Shewana3_0111 at 127.947 kb on + strand, within Shewana3_0111 at 127.947 kb on + strand, within Shewana3_0111 at 127.947 kb on + strand, within Shewana3_0111 at 127.986 kb on + strand, within Shewana3_0111 at 127.986 kb on + strand, within Shewana3_0111 at 128.041 kb on - strand, within Shewana3_0111 at 128.041 kb on - strand, within Shewana3_0111 at 128.097 kb on + strand, within Shewana3_0111 at 128.105 kb on - strand, within Shewana3_0111 at 128.110 kb on - strand, within Shewana3_0111 at 128.379 kb on - strand at 128.609 kb on - strand at 128.769 kb on - strand, within Shewana3_0112 at 128.785 kb on + strand, within Shewana3_0112 at 128.807 kb on - strand, within Shewana3_0112 at 128.836 kb on + strand, within Shewana3_0112 at 128.836 kb on + strand, within Shewana3_0112 at 128.844 kb on - strand, within Shewana3_0112 at 128.857 kb on + strand, within Shewana3_0112 at 128.873 kb on - strand, within Shewana3_0112 at 128.970 kb on + strand, within Shewana3_0112 at 128.994 kb on + strand, within Shewana3_0112 at 129.029 kb on + strand, within Shewana3_0112 at 129.040 kb on + strand, within Shewana3_0112 at 129.141 kb on - strand, within Shewana3_0112 at 129.190 kb on + strand at 129.235 kb on + strand at 129.343 kb on + strand at 129.420 kb on - strand at 129.420 kb on - strand at 129.512 kb on + strand at 129.534 kb on + strand at 129.539 kb on + strand at 129.580 kb on + strand at 129.663 kb on + strand at 129.671 kb on - strand, within Shewana3_0113 at 129.816 kb on + strand, within Shewana3_0113 at 129.957 kb on + strand, within Shewana3_0113 at 129.965 kb on - strand, within Shewana3_0113 at 130.117 kb on + strand, within Shewana3_0113
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 127,666 - Shewana3_0111 0.39 +1.2 127,721 + Shewana3_0111 0.43 +0.8 127,756 - Shewana3_0111 0.46 +0.5 127,767 - Shewana3_0111 0.46 +0.3 127,807 - Shewana3_0111 0.49 +0.2 127,855 - Shewana3_0111 0.53 +0.3 127,892 - Shewana3_0111 0.56 -1.7 127,947 + Shewana3_0111 0.60 +0.9 127,947 + Shewana3_0111 0.60 -0.0 127,947 + Shewana3_0111 0.60 +1.0 127,947 + Shewana3_0111 0.60 -0.1 127,986 + Shewana3_0111 0.63 +0.7 127,986 + Shewana3_0111 0.63 +1.0 128,041 - Shewana3_0111 0.67 -0.0 128,041 - Shewana3_0111 0.67 +0.5 128,097 + Shewana3_0111 0.71 +0.1 128,105 - Shewana3_0111 0.72 -1.0 128,110 - Shewana3_0111 0.72 -2.1 128,379 - +0.5 128,609 - +0.8 128,769 - Shewana3_0112 0.27 +0.4 128,785 + Shewana3_0112 0.29 +0.5 128,807 - Shewana3_0112 0.33 +0.0 128,836 + Shewana3_0112 0.38 -2.2 128,836 + Shewana3_0112 0.38 +0.4 128,844 - Shewana3_0112 0.39 +0.6 128,857 + Shewana3_0112 0.41 +1.3 128,873 - Shewana3_0112 0.44 -3.9 128,970 + Shewana3_0112 0.60 -0.1 128,994 + Shewana3_0112 0.63 +0.3 129,029 + Shewana3_0112 0.69 -0.0 129,040 + Shewana3_0112 0.71 -1.3 129,141 - Shewana3_0112 0.87 +0.4 129,190 + +1.5 129,235 + +0.7 129,343 + +0.3 129,420 - +0.8 129,420 - -0.7 129,512 + +0.2 129,534 + -0.8 129,539 + +0.1 129,580 + +0.4 129,663 + +0.1 129,671 - Shewana3_0113 0.11 -2.2 129,816 + Shewana3_0113 0.34 +0.8 129,957 + Shewana3_0113 0.57 +0.5 129,965 - Shewana3_0113 0.58 -0.0 130,117 + Shewana3_0113 0.83 +0.3
Or see this region's nucleotide sequence