Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4331 and Shewana3_4332 are separated by 205 nucleotides Shewana3_4332 and Shewana3_4333 are separated by 54 nucleotides Shewana3_4333 and Shewana3_4334 are separated by 31 nucleotides Shewana3_4334 and Shewana3_4335 are separated by 27 nucleotides
Shewana3_4331: Shewana3_4331 - methionine sulfoxide reductase A (RefSeq), at 221,669 to 222,331
_4331
Shewana3_4332: Shewana3_4332 - hypothetical protein (RefSeq), at 222,537 to 222,782
_4332
Shewana3_4333: Shewana3_4333 - methionine-R-sulfoxide reductase (RefSeq), at 222,837 to 223,277
_4333
Shewana3_4334: Shewana3_4334 - glutaredoxin (RefSeq), at 223,309 to 223,545
_4334
Shewana3_4335: Shewana3_4335 - peptide methionine sulfoxide reductase (RefSeq), at 223,573 to 224,061
_4335
Position (kb) 222
223 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 221.589 kb on + strand at 221.589 kb on + strand at 221.597 kb on - strand at 221.710 kb on + strand at 221.720 kb on + strand at 221.728 kb on - strand at 221.728 kb on - strand at 221.728 kb on - strand at 222.011 kb on + strand, within Shewana3_4331 at 222.029 kb on - strand, within Shewana3_4331 at 222.081 kb on + strand, within Shewana3_4331 at 222.098 kb on - strand, within Shewana3_4331 at 222.275 kb on - strand at 222.298 kb on - strand at 222.364 kb on + strand at 222.616 kb on - strand, within Shewana3_4332 at 222.641 kb on + strand, within Shewana3_4332 at 222.641 kb on + strand, within Shewana3_4332 at 222.763 kb on - strand at 222.763 kb on - strand at 222.822 kb on - strand at 222.865 kb on - strand at 222.890 kb on + strand, within Shewana3_4333 at 222.890 kb on + strand, within Shewana3_4333 at 222.898 kb on - strand, within Shewana3_4333 at 222.898 kb on - strand, within Shewana3_4333 at 222.988 kb on + strand, within Shewana3_4333 at 223.010 kb on - strand, within Shewana3_4333 at 223.092 kb on + strand, within Shewana3_4333 at 223.239 kb on - strand at 223.673 kb on + strand, within Shewana3_4335 at 223.681 kb on - strand, within Shewana3_4335 at 223.782 kb on - strand, within Shewana3_4335
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 221,589 + -1.6 221,589 + +0.8 221,597 - +0.4 221,710 + +0.4 221,720 + -1.4 221,728 - -1.0 221,728 - -2.7 221,728 - +0.9 222,011 + Shewana3_4331 0.52 +0.9 222,029 - Shewana3_4331 0.54 -0.2 222,081 + Shewana3_4331 0.62 -2.6 222,098 - Shewana3_4331 0.65 -0.1 222,275 - +2.3 222,298 - +0.6 222,364 + +1.3 222,616 - Shewana3_4332 0.32 +0.7 222,641 + Shewana3_4332 0.42 +0.5 222,641 + Shewana3_4332 0.42 +0.5 222,763 - +0.6 222,763 - -2.5 222,822 - +0.0 222,865 - +0.1 222,890 + Shewana3_4333 0.12 -1.9 222,890 + Shewana3_4333 0.12 -0.3 222,898 - Shewana3_4333 0.14 +0.0 222,898 - Shewana3_4333 0.14 +0.2 222,988 + Shewana3_4333 0.34 -2.1 223,010 - Shewana3_4333 0.39 -0.2 223,092 + Shewana3_4333 0.58 -0.4 223,239 - +0.1 223,673 + Shewana3_4335 0.20 +0.2 223,681 - Shewana3_4335 0.22 +0.2 223,782 - Shewana3_4335 0.43 -3.4
Or see this region's nucleotide sequence