Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4320 and Shewana3_4321 overlap by 4 nucleotides Shewana3_4321 and Shewana3_4323 are separated by 85 nucleotides
Shewana3_4320: Shewana3_4320 - signal peptidase II (RefSeq), at 209,919 to 210,998
_4320
Shewana3_4321: Shewana3_4321 - no description, at 210,995 to 213,400
_4321
Shewana3_4323: Shewana3_4323 - MerR family transcriptional regulator (RefSeq), at 213,486 to 213,926
_4323
Position (kb) 210
211
212
213
214 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 210.099 kb on + strand, within Shewana3_4320 at 210.177 kb on - strand, within Shewana3_4320 at 210.190 kb on + strand, within Shewana3_4320 at 210.325 kb on - strand, within Shewana3_4320 at 210.552 kb on + strand, within Shewana3_4320 at 210.646 kb on + strand, within Shewana3_4320 at 210.832 kb on - strand, within Shewana3_4320 at 210.965 kb on - strand at 211.018 kb on - strand at 211.091 kb on + strand at 211.156 kb on - strand at 211.172 kb on - strand at 211.227 kb on - strand at 211.283 kb on + strand, within Shewana3_4321 at 211.291 kb on - strand, within Shewana3_4321 at 211.307 kb on - strand, within Shewana3_4321 at 211.377 kb on - strand, within Shewana3_4321 at 211.390 kb on + strand, within Shewana3_4321 at 211.474 kb on + strand, within Shewana3_4321 at 211.538 kb on + strand, within Shewana3_4321 at 211.573 kb on - strand, within Shewana3_4321 at 211.738 kb on + strand, within Shewana3_4321 at 211.875 kb on + strand, within Shewana3_4321 at 211.912 kb on - strand, within Shewana3_4321 at 211.950 kb on - strand, within Shewana3_4321 at 211.954 kb on - strand, within Shewana3_4321 at 212.011 kb on + strand, within Shewana3_4321 at 212.011 kb on + strand, within Shewana3_4321 at 212.099 kb on - strand, within Shewana3_4321 at 212.198 kb on + strand, within Shewana3_4321 at 212.356 kb on + strand, within Shewana3_4321 at 212.432 kb on - strand, within Shewana3_4321 at 212.501 kb on - strand, within Shewana3_4321 at 212.724 kb on + strand, within Shewana3_4321 at 212.926 kb on - strand, within Shewana3_4321 at 213.090 kb on - strand, within Shewana3_4321 at 213.151 kb on + strand, within Shewana3_4321 at 213.164 kb on - strand at 213.379 kb on + strand at 213.379 kb on + strand at 213.531 kb on + strand, within Shewana3_4323 at 213.569 kb on + strand, within Shewana3_4323 at 213.706 kb on + strand, within Shewana3_4323 at 213.714 kb on - strand, within Shewana3_4323 at 213.716 kb on + strand, within Shewana3_4323 at 213.728 kb on + strand, within Shewana3_4323 at 213.736 kb on - strand, within Shewana3_4323 at 213.793 kb on - strand, within Shewana3_4323 at 213.841 kb on + strand, within Shewana3_4323 at 213.841 kb on + strand, within Shewana3_4323 at 213.849 kb on - strand, within Shewana3_4323 at 214.076 kb on - strand at 214.177 kb on + strand at 214.222 kb on + strand at 214.224 kb on + strand at 214.224 kb on + strand at 214.224 kb on + strand at 214.229 kb on + strand at 214.288 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 210,099 + Shewana3_4320 0.17 +1.2 210,177 - Shewana3_4320 0.24 +0.1 210,190 + Shewana3_4320 0.25 +0.4 210,325 - Shewana3_4320 0.38 +0.6 210,552 + Shewana3_4320 0.59 -2.6 210,646 + Shewana3_4320 0.67 +0.8 210,832 - Shewana3_4320 0.85 +0.1 210,965 - +0.1 211,018 - +0.3 211,091 + -1.8 211,156 - -1.8 211,172 - -1.8 211,227 - -2.0 211,283 + Shewana3_4321 0.12 +1.1 211,291 - Shewana3_4321 0.12 +0.2 211,307 - Shewana3_4321 0.13 -2.0 211,377 - Shewana3_4321 0.16 +0.8 211,390 + Shewana3_4321 0.16 +0.2 211,474 + Shewana3_4321 0.20 +0.6 211,538 + Shewana3_4321 0.23 -1.8 211,573 - Shewana3_4321 0.24 -2.6 211,738 + Shewana3_4321 0.31 +0.8 211,875 + Shewana3_4321 0.37 +0.1 211,912 - Shewana3_4321 0.38 -0.1 211,950 - Shewana3_4321 0.40 -2.0 211,954 - Shewana3_4321 0.40 +0.3 212,011 + Shewana3_4321 0.42 -2.5 212,011 + Shewana3_4321 0.42 +0.1 212,099 - Shewana3_4321 0.46 -0.0 212,198 + Shewana3_4321 0.50 -0.5 212,356 + Shewana3_4321 0.57 +0.4 212,432 - Shewana3_4321 0.60 +0.7 212,501 - Shewana3_4321 0.63 -0.0 212,724 + Shewana3_4321 0.72 -2.3 212,926 - Shewana3_4321 0.80 -1.6 213,090 - Shewana3_4321 0.87 -0.9 213,151 + Shewana3_4321 0.90 -0.2 213,164 - -1.6 213,379 + +0.3 213,379 + +0.3 213,531 + Shewana3_4323 0.10 +0.5 213,569 + Shewana3_4323 0.19 +0.1 213,706 + Shewana3_4323 0.50 +0.2 213,714 - Shewana3_4323 0.52 +0.5 213,716 + Shewana3_4323 0.52 +0.9 213,728 + Shewana3_4323 0.55 -1.3 213,736 - Shewana3_4323 0.57 +1.2 213,793 - Shewana3_4323 0.70 +0.3 213,841 + Shewana3_4323 0.80 -1.9 213,841 + Shewana3_4323 0.80 -0.1 213,849 - Shewana3_4323 0.82 +0.5 214,076 - -2.1 214,177 + +0.1 214,222 + +0.3 214,224 + -3.9 214,224 + -1.7 214,224 + +2.5 214,229 + +1.3 214,288 - +0.9
Or see this region's nucleotide sequence