Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4244 and Shewana3_4245 are separated by 282 nucleotides Shewana3_4245 and Shewana3_4246 overlap by 4 nucleotides
Shewana3_4244: Shewana3_4244 - HsdR family type I site-specific deoxyribonuclease (RefSeq), at 117,132 to 120,386
_4244
Shewana3_4245: Shewana3_4245 - mobile mystery protein B (RefSeq), at 120,669 to 121,280
_4245
Shewana3_4246: Shewana3_4246 - XRE family transcriptional regulator (RefSeq), at 121,277 to 121,765
_4246
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 120.000 kb on - strand, within Shewana3_4244 at 120.027 kb on + strand, within Shewana3_4244 at 120.035 kb on - strand, within Shewana3_4244 at 120.407 kb on - strand at 120.542 kb on + strand at 120.558 kb on + strand at 120.592 kb on - strand at 120.743 kb on - strand, within Shewana3_4245 at 120.798 kb on + strand, within Shewana3_4245 at 120.806 kb on + strand, within Shewana3_4245 at 120.928 kb on - strand, within Shewana3_4245 at 120.928 kb on - strand, within Shewana3_4245 at 121.022 kb on + strand, within Shewana3_4245 at 121.120 kb on - strand, within Shewana3_4245 at 121.152 kb on + strand, within Shewana3_4245 at 121.257 kb on - strand at 121.972 kb on - strand at 122.091 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 120,000 - Shewana3_4244 0.88 -2.8 120,027 + Shewana3_4244 0.89 +0.1 120,035 - Shewana3_4244 0.89 -0.8 120,407 - +0.4 120,542 + +0.4 120,558 + -3.3 120,592 - -2.1 120,743 - Shewana3_4245 0.12 +0.6 120,798 + Shewana3_4245 0.21 +0.2 120,806 + Shewana3_4245 0.22 +0.0 120,928 - Shewana3_4245 0.42 -2.0 120,928 - Shewana3_4245 0.42 -0.6 121,022 + Shewana3_4245 0.58 -2.3 121,120 - Shewana3_4245 0.74 +0.4 121,152 + Shewana3_4245 0.79 -1.2 121,257 - +0.8 121,972 - -1.1 122,091 - +0.2
Or see this region's nucleotide sequence