Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4185 and Shewana3_4186 are separated by 2 nucleotides Shewana3_4186 and Shewana3_4187 are separated by 104 nucleotides Shewana3_4187 and Shewana3_4188 are separated by 287 nucleotides
Shewana3_4185: Shewana3_4185 - hypothetical protein (RefSeq), at 50,898 to 51,413
_4185
Shewana3_4186: Shewana3_4186 - membrane-bound metal-dependent hydrolase (RefSeq), at 51,416 to 51,769
_4186
Shewana3_4187: Shewana3_4187 - hypothetical protein (RefSeq), at 51,874 to 52,488
_4187
Shewana3_4188: Shewana3_4188 - hypothetical protein (RefSeq), at 52,776 to 53,429
_4188
Position (kb) 51
52
53 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 50.966 kb on + strand, within Shewana3_4185 at 51.181 kb on + strand, within Shewana3_4185 at 51.286 kb on - strand, within Shewana3_4185 at 51.406 kb on - strand at 51.489 kb on + strand, within Shewana3_4186 at 51.545 kb on - strand, within Shewana3_4186 at 51.633 kb on + strand, within Shewana3_4186 at 51.855 kb on - strand at 52.172 kb on - strand, within Shewana3_4187 at 52.304 kb on - strand, within Shewana3_4187 at 52.306 kb on + strand, within Shewana3_4187 at 52.529 kb on + strand at 52.821 kb on + strand at 52.827 kb on + strand at 52.835 kb on - strand at 52.839 kb on + strand at 52.858 kb on + strand, within Shewana3_4188 at 52.891 kb on + strand, within Shewana3_4188 at 52.899 kb on - strand, within Shewana3_4188 at 52.899 kb on - strand, within Shewana3_4188 at 52.901 kb on + strand, within Shewana3_4188 at 53.020 kb on - strand, within Shewana3_4188 at 53.020 kb on - strand, within Shewana3_4188 at 53.039 kb on - strand, within Shewana3_4188 at 53.150 kb on + strand, within Shewana3_4188 at 53.158 kb on - strand, within Shewana3_4188 at 53.158 kb on - strand, within Shewana3_4188 at 53.175 kb on + strand, within Shewana3_4188 at 53.299 kb on - strand, within Shewana3_4188
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 50,966 + Shewana3_4185 0.13 -3.3 51,181 + Shewana3_4185 0.55 +0.7 51,286 - Shewana3_4185 0.75 +0.2 51,406 - +0.3 51,489 + Shewana3_4186 0.21 -0.1 51,545 - Shewana3_4186 0.36 +0.3 51,633 + Shewana3_4186 0.61 +0.6 51,855 - -0.1 52,172 - Shewana3_4187 0.48 -1.6 52,304 - Shewana3_4187 0.70 -2.1 52,306 + Shewana3_4187 0.70 -2.2 52,529 + -0.4 52,821 + +0.4 52,827 + -1.1 52,835 - +0.8 52,839 + +0.1 52,858 + Shewana3_4188 0.13 -2.2 52,891 + Shewana3_4188 0.18 +0.8 52,899 - Shewana3_4188 0.19 -1.4 52,899 - Shewana3_4188 0.19 -0.5 52,901 + Shewana3_4188 0.19 +0.6 53,020 - Shewana3_4188 0.37 +0.4 53,020 - Shewana3_4188 0.37 +0.2 53,039 - Shewana3_4188 0.40 -2.2 53,150 + Shewana3_4188 0.57 +0.5 53,158 - Shewana3_4188 0.58 -1.9 53,158 - Shewana3_4188 0.58 +0.4 53,175 + Shewana3_4188 0.61 +0.1 53,299 - Shewana3_4188 0.80 +0.4
Or see this region's nucleotide sequence