Experiment: CDM1 without Lysine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15690 and BBR_RS20920 are separated by 154 nucleotides BBR_RS20920 and BBR_RS15695 overlap by 102 nucleotides
BBR_RS15690: BBR_RS15690 - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase PurH, at 1,288,882 to 1,290,519
_RS15690
BBR_RS20920: BBR_RS20920 - hypothetical protein, at 1,290,674 to 1,290,976
_RS20920
BBR_RS15695: BBR_RS15695 - membrane channel protein, at 1,290,875 to 1,291,813
_RS15695
Position (kb) 1290
1291 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1290.643 kb on - strand at 1290.643 kb on - strand at 1290.677 kb on - strand at 1290.709 kb on + strand, within BBR_RS20920 at 1290.710 kb on + strand, within BBR_RS20920 at 1290.715 kb on + strand, within BBR_RS20920 at 1290.715 kb on + strand, within BBR_RS20920 at 1290.723 kb on - strand, within BBR_RS20920 at 1290.723 kb on - strand, within BBR_RS20920 at 1290.729 kb on + strand, within BBR_RS20920 at 1290.763 kb on + strand, within BBR_RS20920 at 1290.775 kb on + strand, within BBR_RS20920 at 1290.861 kb on - strand, within BBR_RS20920 at 1290.965 kb on + strand at 1290.972 kb on + strand at 1290.992 kb on - strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.127 kb on - strand, within BBR_RS15695 at 1291.127 kb on - strand, within BBR_RS15695 at 1291.198 kb on - strand, within BBR_RS15695 at 1291.250 kb on + strand, within BBR_RS15695 at 1291.258 kb on - strand, within BBR_RS15695 at 1291.373 kb on - strand, within BBR_RS15695 at 1291.379 kb on - strand, within BBR_RS15695 at 1291.446 kb on + strand, within BBR_RS15695 at 1291.510 kb on - strand, within BBR_RS15695 at 1291.561 kb on - strand, within BBR_RS15695 at 1291.561 kb on - strand, within BBR_RS15695 at 1291.658 kb on + strand, within BBR_RS15695 at 1291.658 kb on + strand, within BBR_RS15695 at 1291.677 kb on + strand, within BBR_RS15695 at 1291.682 kb on + strand, within BBR_RS15695 at 1291.697 kb on + strand, within BBR_RS15695 at 1291.710 kb on - strand, within BBR_RS15695 at 1291.720 kb on - strand at 1291.727 kb on - strand at 1291.735 kb on - strand at 1291.763 kb on - strand at 1291.791 kb on - strand at 1291.872 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Lysine remove 1,290,643 - +0.0 1,290,643 - -1.2 1,290,677 - -3.4 1,290,709 + BBR_RS20920 0.12 -2.5 1,290,710 + BBR_RS20920 0.12 -2.9 1,290,715 + BBR_RS20920 0.14 -1.9 1,290,715 + BBR_RS20920 0.14 -3.4 1,290,723 - BBR_RS20920 0.16 -2.3 1,290,723 - BBR_RS20920 0.16 -2.2 1,290,729 + BBR_RS20920 0.18 -1.8 1,290,763 + BBR_RS20920 0.29 -3.0 1,290,775 + BBR_RS20920 0.33 -3.5 1,290,861 - BBR_RS20920 0.62 -2.3 1,290,965 + +0.2 1,290,972 + +0.1 1,290,992 - BBR_RS15695 0.12 +1.0 1,291,041 + BBR_RS15695 0.18 -1.4 1,291,041 + BBR_RS15695 0.18 -0.4 1,291,041 + BBR_RS15695 0.18 +0.4 1,291,041 + BBR_RS15695 0.18 -1.8 1,291,041 + BBR_RS15695 0.18 +1.2 1,291,041 + BBR_RS15695 0.18 -1.3 1,291,041 + BBR_RS15695 0.18 +1.2 1,291,127 - BBR_RS15695 0.27 -0.9 1,291,127 - BBR_RS15695 0.27 -0.6 1,291,198 - BBR_RS15695 0.34 -1.0 1,291,250 + BBR_RS15695 0.40 +0.7 1,291,258 - BBR_RS15695 0.41 +0.5 1,291,373 - BBR_RS15695 0.53 -0.8 1,291,379 - BBR_RS15695 0.54 +1.0 1,291,446 + BBR_RS15695 0.61 +0.4 1,291,510 - BBR_RS15695 0.68 +0.1 1,291,561 - BBR_RS15695 0.73 +0.6 1,291,561 - BBR_RS15695 0.73 -1.6 1,291,658 + BBR_RS15695 0.83 -2.2 1,291,658 + BBR_RS15695 0.83 -0.6 1,291,677 + BBR_RS15695 0.85 +0.7 1,291,682 + BBR_RS15695 0.86 -0.9 1,291,697 + BBR_RS15695 0.88 -0.0 1,291,710 - BBR_RS15695 0.89 +0.7 1,291,720 - +0.7 1,291,727 - -1.0 1,291,735 - -0.0 1,291,763 - +0.2 1,291,791 - -2.3 1,291,872 + -0.2
Or see this region's nucleotide sequence